JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (3): 30-35.doi: 10.6040/j.issn.1671-7554.0.2015.842

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Screening crucial genes for glucose metabolism in rheumatoid arthritis

ZHAO Yan1,2, ZHENG Yabing2, YAN Xinfeng3, ZHANG Hu3, CHANG Xiaotian2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Medical Research Center, Qianfoshan Hospital, Shandong University, Jinan 250014, Shandong, China;
    3. Department of Orthopedics, Qianfoshan Hospital, Shandong University, Jinan 250014, Shandong, China
  • Received:2015-09-06 Online:2016-03-10 Published:2016-03-10

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Abstract: Objective To determine the crucial enzymes of glucose metabolism in rheumatoid arthritis(RA). Methods The glucose metabolism in synovial tissues of collagen-induced arthritis(CIA)was analyzed with Rat Glucose Metabolism RT2 ProfilerTM PCR Array. The result was verified using real time PCR, Western blotting and ELISA in synovial tissues and blood from patients with RA. Results The PCR array detected up-regulation of ENO1, HK2 and PGK1, and down-regulation of PCK1 and PDK4 in synovial tissues of CIA rats. Real time PCR and Western blotting detected increased expressions of ENO1 and PGK1 in synovial tissues of RA. ELISA detected a high level of PGK1 in blood of RA patients. The result identified the increased expression of ENO1 and PGK1 in CIA and RA synovial tissues, and elevated level of PGK1 in blood of the patients with RA. Conclusion Glycolytic activity is increased in RA, which may result in the abnormal expression of genes involved.

Key words: Rheumatoid arthritis, Collagen-inducing arthritis, Enolase1(alpha), Glucosemetabolism, Phosphoglycerate kinase 1

CLC Number: 

  • R593.22
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