JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (1): 63-68.doi: 10.6040/j.issn.1671-7554.0.2016.1312

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Expression of microRNA-200b in granulosa cells of PCOS patients and its significance

JIA Yueyue, LIU Hongbin, LI Jingbo, LI Jing, ZHANG Jiangtao, SUN Mei, SHI Yuhua   

  1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    Key Laboratory for Reproductive Endocrinology(Shandong University), Ministry of Education;
    Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, Shandong, China
  • Received:2016-10-14 Online:2017-01-10 Published:2017-01-10

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Abstract: Objective To explore the expression and clinicopathological significance of microRNA-200b(miR-200b)in ovarian granulosa cells of patients suffering from polycystic ovary syndrome(PCOS)with insulin resistance. Methods A total of 120 PCOS patients(78 with insulin resistance and 42 without)and 72 controls were enrolled. The expression of miR-200b was examined in ovarian granulosa cells by real-time quantitative reverse transcription PCR(qRT-PCR), and the clinical significance was analyzed. Results PCOS patients with insulin resistance had higher morbidity of overweight, obesity, hypertensive disease, hyperglycemia, dyslipidemia and metabolic syndrome than PCOS patients without insulin resistance and controls(all P<0.05). Expression of miR-200b was much higher in PCOS patients with insulin resistance than in PCOS patients without insulin resistance and controls(P=0.012, P<0.001). In PCOS 山 东 大 学 学 报 (医 学 版)55卷1期 -贾月月,等.多囊卵巢综合征患者颗粒细胞microRNA-200b的表达及影响 \=-patients with insulin resistance, the index of homeostatic model assessment of insulin resistance(HOMA-IR)was positively correlated with miR-200b expression(r=0.532, P=0.002). Conclusion PCOS patients with insulin resistance have high morbidity of long-term metabolic complications. The high expression of miR-200b is positively related to insulin resistance, which may be the underlying pathogenic mechanism for insulin resistance in PCOS patients.

Key words: Granulosa cell, microRNA-200b, Insulin resistance, Polycystic ovary syndrome

CLC Number: 

  • R711.75
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