JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (7): 8-12.doi: 10.6040/j.issn.1671-7554.0.2015.375

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Therapeutic effects of human osteoblast-stimulating factor on osteoporosis of ovariectomized rats

FENG Xiaoyu1, ZHANG Hongmei2, CHE Xuanqiang1, KANG Donghong3   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Endocrinology, Central Hospital of Zibo, Zibo 255036, Shandong, China;
    3. Department of Endocrinology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2015-04-13 Revised:2015-05-18 Online:2015-07-10 Published:2015-07-10

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Abstract: Objective To investigate the therapeutic effects of human osteoblast-stimulating factor (OSF-1) on the osteoporosis of ovariectomized rats. Methods A total of 12 female rats aged 4 months were randomly divided into 4 groups: SHAM group, OVX group, human parathyroid hormone group (PTH group) and OSF-1 group. A small piece of adipose tissue was cut off from rats in SHAM group; bilateral ovariectomy was conducted in rats in the other groups. All rats were intervened per day separately since 10 weeks after the ovariectomy: SHAM and OVX groups received subcutaneous injection of 0.2 mL/d sodium chloride solution, PTH group was given subcutaneous injection of 20 μg/(kg·d) PTH, and OSF-1 group was injected subcutaneously with 80 μg/(kg·d) OSF-1. Pharmacological agents were administrated consecutively for 11 weeks, then all rats were sacrificed. Uterus was removed and weighed. Femur was removed and cleaned of adhering muscles and connective tissues. Microcomputed tomography (micro-CT) was used to scan proximal femur metaphysis. Results Uterus weight and bone mass in OVX group were significantly lower than those in SHAM group (P<0.01), and the microstructure of bone trabecula was destructed in OVX group. Compared with OVX group, bone morphometric parameters in PTH and OSF-1 groups showed significant increase on bone mineral density(BMD), BV/TV, Tb.N(P<0.01), while Tb.Sp and TBPf were lower (P<0.01, P<0.05),and bone trabecula were more conspicuous in PTH and OSF-1 groups. The parameters of BMD, BV/TV and Tb.N in PTH group were higher than those in OSF-1 group (P<0.01), and TBPf was lower than that in OSF-1 group (P<0.01). Conclusion Subcutaneous injection of 80 μg/(kg·d) OSF-1 can increase bone mass and improve bone microstructure in ovariectomized rats.

Key words: Microcomputed tomography, Ovariectomized rats, Human osteoblast-stimulating factor, Postmenopausal osteoporosis

CLC Number: 

  • R589
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