Table of Content

  

10 August 2015
Volume 53 Issue 8

Effect of Lobelia alkaloids on endothelin-1 signal pathway in a rat model of pulmonary arterial hypertension

LIU Huimin, LIU Deng, LI Xiaoyu, ZOU Shufeng, JIANG Limin, LI Yuhuan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  1-4.  doi:10.6040/j.issn.1671-7554.0.2014.905

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Objective To observe the effect of Lobelia alkaloids on pulmonary vascular remodeling of pulmonary arterial hypertension (PAH) rat model and to explore its mechanism. Methods Twenty-four male Wistar rats were randomly divided into normal control group, model group and Lobelia alkaloids group. PAH rat models were established by giving monocrotaline (MCT) intraperitoneally, and the rats were given Lobelia alkaloids by intragastric administration for 4 weeks. HE staining, ELISA and immunohistochemical methods were used to study pulmonary morphological changes, endothelin-1 (ET-1) level in plasma and endothelin receptor A (ET_A) expression in lung tissue. Results Lobelia alkaloids could significantly alleviate pulmonary artery remodeling and decrease the ET-1 level in plasma and ET_A protein expression in lung tissue of PAH rat model. Conclusion Lobelia alkaloids can inhibit significantly pulmonary artery remodeling of PAH rat model induced by MCT which may be related to the decreased ET-1 level in plasma and ET_A protein expression in lung tissue.

Effects of 1,25(OH)₂D₃ on the induction of immune tolerance in Lewis rats with EAMG

LI Xiuhua, LI Xiaoli, DUAN Ruisheng, ZHU Meijia, CAO Lili, LI Yanbin, WANG Si, YUE Longtao, MA Qinghai, LIU Fei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  5-10.  doi:10.6040/j.issn.1671-7554.0.2015.266

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Objective To investigate the effects of 1,25(OH)₂D₃ on the induction of immune tolerance in Lewis rats with experimental autoimmune myasthenia gravis (EAMG). Methods EAMG models were established by subcutaneous injection of synthetic peptide R97-116 into both hind footpads of Lewis rats. The models were then divided into 1,25(OH)₂D₃ treatment group (experimental group) and control group. The clinical symptoms were evaluated. The expressions of CD80 and CD86 on MNC and Treg cells of lymph node mononuclear cells (MNC) were determined with flow cytometry. Cell proliferation was analyzed with CCK-8 test. Cell cycle was detected with flow cytometry. Cyto-kines of MNC supernatants were determined with ELISA. Serum anti-R97-116 IgG antibody was detected with ELISA. Results Compared with the control group, the experimental group exhibited lower clinical scores 28 d after first immunization (P <0.01) and less loss of body weight 35 d after first immunization (P <0.01); lower levels of CD80 and CD86 on MNC (P <0.05); higher percentages of CD4⁺CD25⁺ T cells and CD4⁺Foxp3⁺ T cells among lymph node MNC (P <0.01, P< 0.05); decreased lymphocyte proliferation in the presence of R97-116 antigen (P< 0.01); lower percentage of cells of S phase and of S phase plus G2/M phase (both P <0.05); higher level of IL-4 and lower levels of IFN-γ as well as IL-17 (all P <0.05); lower level of anti-rat R97-116 IgG antibody (P <0.05). Conclusion 1,25(OH)₂D₃ could ameliorate EAMG by down-regulating the expressions of CD80 and CD86 on MNC, up-regulating the number and function of Treg cells, inhibiting lymphocyte proliferation, changing the lymphocyte cell cycle, shifting cytokine profile from Th1/Th17 to Th2 type cytokines, and decreasing level of anti-R97-116 IgG antibody in serum.

Construction of an eukaryotic vector of rhoptry protein 7 from Toxoplasma gondii and its immunoprotective effect

WANG Lin, LÜ Gang, HAN Yali, GUO Jingjing, ZHAO Qunli, HE Shenyi

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  11-15.  doi:10.6040/j.issn.1671-7554.0.2014.969

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Objective To construct the eukaryotic expression vector of rhoptry protein 7 (ROP7) of Toxoplasma gondii and to evaluate the protective effect induced by recombinant plasmid on mice. Methods PCR primers were designed based on the ROP7 sequence of Toxoplasma gondii in Genbank. The ROP7 gene was amplified by PCR and inserted into the eukaryotic vector pEGFP-C1 and the recombinant plasmid pEGFP-C1/ROP7 (pROP7) was constructed. Then the plasmids were transfected into HEK293T cells to verify the expression in eukaryotic cells. A total of 36 female BALB/c mice were randomly divided into three groups: phosphate buffer saline (PBS) control group, pEGFP-C1 control group and the pROP7 experimental group. Then PBS, pEGFP-C1 and pROP7 were used to immunize the corresponding group of mice respectively. The special serum IgG level was detected with ELISA and the survival time of challenged mice was recorded to evaluate the immune protection. Results The target gene (1728bp) was successfully amplified by PCR. The recombinant plasmid was successfully constructed and expressed in HEK293T cells. Its protein was recognized with a goat anti-Toxopasma gondii antibody. Mice immunized with the recombinant plasmid produced high levels of serum antibodies. In the challenge experiment, the survival time of mice in pROP7 experience group was significantly prolonged compared with that of control groups (P <0.05). Conclusion With the immunological effect of this expressed recombinant plasmid, the present study might provide reference for future study of DNA vaccine against Toxoplasma gondii.

Prestin expression in outer hair cells of Spag6 gene knockout mice

WANG Jinghan, LI Xiaofei, ZHANG Zhibing, WANG Haibo, LI Jianfeng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  16-22.  doi:10.6040/j.issn.1671-7554.0.2014.665

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Objective To detect the prestin expression in sperm associated antigen 6 (Spag6) gene knockout mice and its possible relationship with SPAG6. Methods Through a confocal microscope, the isolated basilar membrane was immunostained for observing the distribution of prestin and SPAG6 in hair cells. In particular, the fluorescence intensity of prestin and the morphological alterations of hair cells were compared among wild type mice (Spag6+/+), heterozygous mice (Spag6+/-) and the corresponding Spag6 gene knockout littermates (Spag6-/-). The expressions of prestin protein and mRNA in mice of three genotypes were measured by Western blotting and real-time Q-PCR. Results SPAG6, which was positive at the cuticular plate and co-localized with prestin at the cell lateral wall, expressed in outer hair cells (OHCs) of both wild type (Spag6+/+) and heterozygous (Spag6+/-) adult mice. Compared with wild type (Spag6+/+) mice, gene knockout mice (Spag6-/-) showed the lower fluorescence intensity of prestin and apparent morphological abnormity of OHCs. The expressions of prestin at protein and mRNA levels in gene knockout mice (Spag6-/-) were significantly decreased as evidenced by Western blotting and real-timeQ-PCR. Conclusion SPAG6 participates in the formation of the cell membrane of OHCs and affects the expression of prestin in OHCs, which implies a possible interaction of these two proteins.

Protective effect of mitochondria-targeted peptide SS-31 against H₂O₂-induced apoptosis in human lens epithelial cells

GUO Changyan, QIN Xuejiao, WU Xiaoli, GAO Xue, CAI Keli

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  23-26.  doi:10.6040/j.issn.1671-7554.0.2014.195

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Objective To study the protective effect of SS-31 against H₂O₂-induced apoptosis in human lens epithelial cells and the possible mechanism. Methods SRA01/04 cells, an immortal human lens epithelial cell line, were cultured in vitro. SRA01/04 cells at the same generation were chosen and 100 μmol/L H₂O₂ was used to build the model of apoptosis. The cells were randomly allocated into five groups: the control group, the group treated by H₂O₂, and three groups pretreated by different concentrations of SS-31. The cell viabilities were monitored by using 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide(MTT) assay. The cell apoptotic rates were detected with Annexin V-Propidium iodide double-staining flow cytometry. The expressions of Bcl-2, Bax and Cleaved-Caspase-3 were tested by using Western blotting. Results After exposure of 100 μmol/L H₂O₂ for 24 h, the cell viabilities decreased, cell apoptotic rates increased, expression of pro-apoptotic protein Bax increased, expression of anti-apoptotic protein Bcl-2 decreased, and expression of Cleaved-Caspase-3 increased. Pretreatment of SS-31 at different concentrations improved cell viabilities in a dose-dependent manner. Meanwhile, SS-31 plays an anti-apoptotic role by decreasing cell apoptotic rate, down-regulating the expression of Bax, up-regulating the expression of Bcl-2, and suppressing the activation of Cleaved-Caspase-3. Conclusion SS-31 efficiently protects human lens epithelial cells against H₂O₂-induced apoptosis. Further studies are needed on whether SS-31 can be used in medicine treatment for cataract.

Protective effect of riluzole on the damage of rat retina induced by acute intraocular hypertension

ZHANG Nan, ZHOU Lixiao, SUN Chenglin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  27-31.  doi:10.6040/j.issn.1671-7554.0.2015.238

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Objective To investigate the protective effect of riluzole on the damage of rats' retinal ganglion cells induced by acute intraocular hypertension. Methods A total of 120 SD rats were randomly divided into control group, high intraocular pressure group, and riluzole intervention group, 40 in each group. Acute ocular hypertention rats model, which were established using the anterior chamber perfusion way, were injected intraperitoneally with riluzole. After 12, 24, 48 and 72 h of reperfusion, the expression of Caspase-3 protein and the levels of apoptosis in each layer of rats' retinal cells were detected by immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). The structural changes of the rats' retina in each group were observed by HE staining. Results Compared with the high intraocular pressure group, the thickening and structural disorder in riluzole intervention group were relieved, and the expression of Caspase-3 and the apoptosis cells at different time points in riluzole intervention group decreased (P< 0.05). Conclusion Riluzole can reduce the apoptosis of retinal cells in rats with damaged retina caused by acute ocular hypertention and has significant protective effects to retina.

Antibacterial activity of acellular dermal matrix composite CMC

AN Xianglian, GUO Jing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  32-37.  doi:10.6040/j.issn.1671-7554.0.2014.755

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Objective To explore the antibacterial activity of acellular dermal matrix (ADM) with glutaraldehyde as crosslinking agent composite carboxymethyl chitosan(CMC). Methods The 2×3 factorial design was adopted, and the experiments were designed according to the sample status (wet, dry) and groups (composite sample group, simple sample group, blank control group). Shaking-flask method was used and the optimized experimental conditions (rotational speed 200 r/min, culture temperature 25 ℃, culture time one hour) were used to evaluate the antibacterial activity of acellular dermal matrix composite CMC. To get the bacterial inhibition rate, colony plate count was adopted to obtain the number of bacterial colonies before the experiment(0 h) and after the test(1 h). In the two experimental groups, the number of bacteria accorded with the qualified standard before and after the shaking-flask (change of the number was no more than 10%). Results In wet condition, the antibacterial rate of composite sample group was 61.17% for staphylcoccus aureus, and 39.79% for candida albicans. In dry condition, the antibacterial rate of composite sample group was 0% for staphylcoccus aureus, and 15.17% for candida albicans. There were statistical differences (P <0.001). Conclusion The crosslinking compound has certain antibacterial activity in wet state. In dry condition, it has no antibacterial activity, and the crosslinking efficiency is lower and needs to be improved.

Comparative study of different biomolecule coatings on the surface of pure titanium disc

ZHANG Feng, DAI Jie, REN Lingfei, JIANG Songyao, SHI Gengsheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  38-43.  doi:10.6040/j.issn.1671-7554.0.2014.824

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Objective To construct different biomolecule coatings on the surface of pure titanium discs and to find out a proper implant coating. Methods Pure titanium disc was first ground, polished and acid-etched with a mixture of H₂SO₄/H₂O₂(acid-etched group, as the control group). Collagen type Ⅰ (COL), chitosan (CHI), hyaluronic acid (HA) and polyglutamic acid (PGA) were introduced onto this acid-etched surface using layer by layer technique (coating group). Scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), quartz crystal microbalance (QCM) and contact angle measurement were used for surface characterization. After pre-osteoblasts were seeded on the discs, cell attachment, proliferation and differentiation were evaluated. Results SEM analysis demonstrated that the surfaces of different coatings were very smooth and similar. XPS analysis showed that the nitrogen content of different surface was dramatically increased with deposition number. Contact angle measurement showed that the construction of different biomolecules on pure titanium discs did not damage the excellent wettability of the basal surface. Cell attachment, proliferation and differentiation were much stimulated in the coating groups when compared with the acid-etched group. On the other hand, COL-based coatings [Ti-(COL/HA)₅-COL and Ti-(COL/PGA)₅-COL] showed much higher bioactivity compared with CHI-based coatings [Ti-(CHI/HA)₅-CHI and Ti-(CHI/PGA)₅-CHI], and Ti-(COL/HA)₅-COL coating promoted cell attachment, proliferation and differentiation much more than those of Ti-(COL/PGA)₅-COL coating. Conclusion Collagen type Ⅰ, chitosan, hyaluronic acid and polyglutamic acid are successfully constructed on the titanium surface, and Ti-(COL/HA)₅-COL coating shows high bioactivity and may promote implant osseointegration.

Application of enterprise stent in atherosclerotic vertebral-basilar arterial stenosis

WANG Xiaofei, WANG Chengwei, WANG Zhigang, DING Xuan, WANG Minqing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  44-48.  doi:10.6040/j.issn.1671-7554.0.2015.153

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Objective To assess the safety and efficacy of enterprise stent in the treatment of atherosclerotic vertebral-basilar arterial stenosis. Methods A retrospective study was performed in patients who received enterprise stent in the Department of Neurosurgery, Second Hospital of Shandong University during Jan. 2012 and June 2014. Patients were prescribed with medication of clopidogrel 75 mg/d and aspirin 300 mg/d at least 3 days preoperatively. The observation indexes, clinical follow-ups and digital subtraction angiography (DSA) performed in month 1, 3, 6 and 12 postoperatively were collected and reviewed. Results Of the 46 patients with 48 lesions, 33 (71.7%) were men and 13 (28.3%) were women, 28 lesions (58.3%) were at the basilar artery, 15 (31.3%) were at the left vertebral artery, and 5 (10.4%) were at the right vertebral artery. The technical success rate was 100%. Altogether 4 patients (8.7%) developed perioperative complications. DSA examination of 35 lesions 6 months postoperatively showed 5 cases (14.3%) had postoperative restenoses. And 5 (10.9%) ischemic events occurred during the follow-up. Conclusion Application of enterprise stent is safe and efficacious, with high technical success rate and low perioperative complication rate.

Curative effect of domestic Wagner SL prosthesis replacement for Evans Ⅲ intertrochanteric fracture in elderly patients

LIU Shenghou, WANG Shaojin, LIU Wenguang, YIN Qingfeng, ZHAO Heng, XIAO Shipeng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  49-52.  doi:10.6040/j.issn.1671-7554.0.2015.431

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Objective To retrospectively analyze the curative effect of domestic Wagner SL prosthesis replacement in elderly patients with Evans Ⅲ intertrochanteric fracture (IF). Methods A total of 17 elderly patients (6 male and 11 female, age 75～84 years, average 79.8 years) with Evans Ⅲ IF who underwent domestic Wagner SL prosthesis replacement, and another 12 controls (4 male and 8 female, age 77～85 years, average 81.1 years) with Evans Ⅲ IF who received cemented long-stem prosthesis replacement were observed. The operation time, intraoperative blood loss, postoperative blood loss, postoperative bed time, postoperative function score of the two groups were compared. Results There were no statistical significance between the two groups in terms of age, sex, body mass index and degree of osteoporosis (P >0.05). The operation time and intraoperative blood loss of Wagner SL prosthesis replacement group was significantly less than that of the control group (P <0.05). There was no statistical significance in the volume of drainage, postoperative bed time and Harris hip scores (P >0.05). Conclusion Domestic Wagner SL prosthesis replacement for elderly patients with comminuted intertrochanteric fracture (CIF) has advantages of minimal invasion, rapid recovery, good efficacy and low hospital expenses. Therefore, it is worth of wide clinical application.

Application of a novel barbed suture in wound closure of hip and knee replacement

ZHAO Heng, YIN Qingfeng, LIU Wenguang, LIU Shenghou, XIAO Shipeng, WANG Shaojin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  53-56.  doi:10.6040/j.issn.1671-7554.0.2015.092

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Objective To determine whether the barbed surgical suture, a new type of knotless and biodegradable suture, was associated with higher efficiency and better healing in hip and knee replacement. Methods A total of 64 total joint arthroplasty (TJA), including 35 total hip arthroplasty (THA) and 29 total knee arthroplasty (TKA) were elected to this prospective and controlled study. The subjects were divided into barbed suture group and conventional suture group randomly according to the odevity of the last figure of the ID number. A designated personnel was responsible to record the operative incision length and its closure time. In the barbed suture group, the deep fascial layer was closed with running stitches of 2 # barbed suture and the subcutaneous layer was closed with running stitches of 0 # barbed suture. In the conventional suture group, the deep fascial layer was closed with interrupted stitches of 1 # biodegradable suture and the subcutaneous layer was closed with interrupted stitches of 0 # biodegradable suture. Two weeks, 6 weeks and 3 months after operation, the patients' healing status of incisions were evaluated and their complications were recorded. In the end, statistical analysis was performed. Results There were no differences between the two groups in terms of age, gender, body mass index (BMI), and length of incision (P=0.74). The closure time of the barbed suture group was significantly shorter than that of conventional suture group (P <0.001). As for suturing speed (time/length), the barbed suture group had an advantage over conventional suture group (P <0.001). In both groups, no serious complications occurred. Meanwhile, there was no significant difference in mild complications (P=0.80). Conclusion Compared to the conventional suture group, barbed suture group can make the wound closure in hip and knee replacements faster and better. And this novel suture is not associated with incidence of complications.

Anthropometric analysis of the size of anterior arch in teenager orthodontic patients with 3D-model

LIU Jianwei, GUO Jing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  57-60.  doi:10.6040/j.issn.1671-7554.0.2014.971

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Objective To observe the size of anterior arch in Han orthodontic patients. Methods A total of 66 teenager orthodontic patients treated in our hospital were enrolled in the research. The 3D digital models and plaster models of all patients were built to measure the height, width and thickness of each anterior tooth. Results The Bolton's ratio showed significant difference between the Mongoloid and Caucasion ethnic groups (P <0.05). The mesiodostal width of maxillary lateral incisor and mandibular central incisor played an important part in Bolton's ratio discrepancies. The thickness of maxillary lateral incisor also displayed a marked difference. The right-left discrepancies in mesiodistal width of maxillary lateral incisor and mandibular central incisor were much more than the other anterior teeth. The significant differences of right-left discrepancies in clinical crown heights were canine. Conclusion The size of anterior teeth in Mongoloid teenager orthodontic patients shows ethnic differences and regularities, which can provide clinical reference for orthodontic treatment.

Different condylar displacements between centric relation and maximum intercuspation before and after orthodontic intervention

HAN Xu, SHAO Yuting, SUN Qinfeng, GUO Jing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  61-65.  doi:10.6040/j.issn.1671-7554.0.2014.447

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Objective To investigate the effects of orthodontic intervention on maximum intercuspation (MIC)- centric relation (CR) condylar displacement of patients with or without temporomandibular disorders (TMD). Methods A total of 31 orthodontic patients aged 16 to 45 years were selected and divided into the TMD group (n=15) and non-TMD group (n=16). Records of MIC and CR of these patients taken before and after orthodontic intervention were compared. Results The two groups had different MIC-CR displacement before and after treatment. There were more changes in the TMD group, and the changes were mostly favorable. The MIC-CR condylar displacement was correlated with the symptom checklist (SCL) score. Conclusion Orthodontic intervention has effect on condylar position of patients, especially for those with TMD. Orthodontists, therefore, need to understand and pay attention to the effect of malocclusion on TMD and limitations of measurement of condylar displacement (MCD) in the diagnosis.

Alveolar bone volume around upper incisors in adult patients with Class Ⅰ bimaxillary protrusion

LIU Lu, LI Rongrong, LIU Mengjun, ZHANG Yan, LIU Dongxu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  66-70.  doi:10.6040/j.issn.1671-7554.0.2015.041

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Objective To investigate the alveolar bone volume around upper incisors in adult patients with Class Ⅰ bimaxillary protrusion and provide a reference for orthodontic diagnosis and treatment planning. Methods Twenty-two cases with Class Ⅰ bimaxillary protrusion (bimaxillary protrusion group) and Eighteen cases with individual normal occlusion (control group) were recruited. Lateral cephalograms and cone beam computed tomography(CBCT) scannings were taken respectively and the alveolar bone volume around four upper incisors in each subject were measured by Mimics 16.0. Results In bimaxillary protrusion group, alveolar bone volume around upper incisors decreased compared to the control group (P <0.05), apart from labial root apex zone of central incisors, labial root apex and palatal crest zone of the lateral incisors. Besides, alveolar crest zone, middle root zone, palatal alveolar bone, total alveolar bone of the central incisors, labial alveolar bone, palatal alveolar bone, total alveolar bone of the lateral incisors and total alveolar bone volume of four upper incisors in bimaxillary protrusion group were decreased compared to the control group (P <0.001). Conclusion The alveolar bone volume around upper incisors in adult patients with Class Ⅰ bimaxillary protrusion is fewer than that in the subjects with normal occlusion. It should be more cautious in the process of treatment planning, especially retraction or intrusion of the upper incisors.

CBCT evaluation of the upper airway morphological changes in skeletal Class Ⅲ malocclusion patients using protraction and rapid maxillary expansion appliance

CHEN Xueling, WU Zizhong, LIU Dongxu, XIE Yongtao, GUO Tiantian, ZHANG Shijie

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  71-78.  doi:10.6040/j.issn.1671-7554.0.2014.636

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Objective To evaluate the effects of protraction headgear with rapid maxillary expansion (PE) on the upper airway morphological dimensions in growing patients with skeletal Class III malocclusion. Methods The pre-treatment (T1) and post-treatment (T2) cone beam computed tomography (CBCT) data of 30 patients aged 8 to 11 years who had completed PE therapy were collected. After the data were reconstructed and registered, the morphological changes of the upper airway were measured. The t-test was used to assess the statistical differences. Results After the PE treatment, the subspinale (A) of maxillary and upper incisor (UI) moved in the anterosuperior direction. The gnathion (Gn) of mandible moved in the posterior-inferior direction. The placement of the hyoid bone, length and width of dentalarch, volume and mean cross-sectional area of nasopharynx and oropharynx region had significant differences, while the hypopharynx showed no remarkable change. The ratio of the largest anteroposterior/lateral diameter of the oropharynx increased, while that of the hypopharynx decreased. Conclusion The PE treatment of Class Ⅲ malocclusion with maxillary skeletal deficiency produces a significant enlargement in the nasopharynx and oropharynx, but the hypopharynx shows no significant changes. The oropharynx is found to be more circular and the hypopharynx becomes more elliptic in transverse shape.

Applications of different mandibulotomy approaches to the resection of pharyngeal region tumors

HUANG Naibang, DA Peng, LU Jun, WU Hao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  79-82.  doi:10.6040/j.issn.1671-7554.0.2014.1009

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Objective To discuss the indications of different mandibulotomy approaches for the exposure and resection of tumors in the pharyngeal region. Methods A retrospective analysis was presented for 23 resection of pharyngeal region tumors between January 2006 and December 2013. The mandibulotomy included the middle of the corpus mandibulae osteotomy (7 cases), the angulus mandibulae osteotomy (6 cases), pure block or “L” shape corpus mandibulae osteotomy (5 cases), and through vertical corpus mandibulae osteotomy (5 cases). Results The operation visual fields were sufficient. The operations were completed smoothly and successfully.The cutting edge of malignant tumors were all negative. Twelve patients were treated with postoperative radiotherapy or radiotherapy companied by chemotherapy, and radioactive osteomyelitis did not occur. All patients' mandibular function recovered well and the degree of mouth opening and occlusion met the ideal requirements. Conclusion Different mandibulotomy approach should be chosen according to the primary tumor location, nature, and size. To take different approaches flexibly can not only acquire a good operative field but also meet the demand of one stage repairment and recovery of stomatognathic system function.

Type 2 diabetes mellitus associated with thyroid nodules: a longitudinal health check-up cohort study

LIU Yanxun, LIU Jia, ZHANG Tao, WANG Lu, XUE Fuzhong, WANG Ping

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  83-86.  doi:10.6040/j.issn.1671-7554.0.2014.721

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Objective To construct a large-scale longitudinal health check-up cohort in urban Chinese population to highlight and confirm the association between type 2 diabetes mellitus and thyroid nodules, to explore the possible factors affecting the formation of thyroid nodule, and to provide guidance for the prevention of thyroid nodules. Methods A longitudinal cohort with 15 528 participants was established based on the routine health check-up systems, and generalized estimating equation (GEE) model was used to detect the association between type 2 diabetes mellitus and thyroid nodules. Results A total of 1 592 cases of thyroid nodules occurred during the 6-year follow-up. The incidence densities of type 2 diabetes mellitus group and healthy group were 36.33 per 1 000 person-years and 28.55 per 1 000 person-years respectively. The difference was statistically significant (χ²=14.80, P <0.001).Our data confirmed and clarified the association between type 2 diabetes mellitus and thyroid nodules (RR=1.299, P= 0.001) after adjusting other potential confounding factors. Conclusion Type 2 diabetes mellitus is associated with thyroid nodules in urban Chinese population.

Status and related factors of aberrant drug-related behaviors in patients with chronic pain

ZHAO Yang, LOU Fenglan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  87-91.  doi:10.6040/j.issn.1671-7554.0.2014.949

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Objective To investigate the status and related factors of aberrant drug-related behaviors in patients with chronic pain. Methods A total of 218 patients with chronic pain from Shandong Provincial Hospital Affiliated to Shandong University and Qilu Hospital of Shandong University were investigated using general information questionnaire, the Current Opioid Misuse Measure (COMM), Brief Pain Inventory (BPI), Pain Catastrophizing Scale (PCS) and the Hospital Anxiety and Depression Scale (HADS). Results The average score of aberrant drug-related behaviors was (11.24±7.80). Stepwise regression analysis showed that age, months of opioid treatment, smoking, rumination, and depression were the independent influencing factors of aberrant drug-related behaviors. Conclusion Medical stuff should have a better understanding of the related factors of aberrant drug-related behaviors in patients with chronic pain and provide individualized assessment and intervention measures in order to achieve excellent therapeutic effect as well as to improve the quality of life.

Burden of malignant neoplasms evaluated by DALYs in Yantai city

YU Shaoyi, CHEN Yuanyin, LIU Haiyun, QU Shu'na, XU Ying, WANG Qianqian, WANG Maobo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(8):  92-96.  doi:10.6040/j.issn.1671-7554.0.2014.621

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Objective To evaluate the burden of malignant neoplasms in Yantai City in order to provide scientific basis for policy-making. Methods The registration data from Yantai cancer registry and mortality surveillance in 2012 were reviewed.Years of life lost with premature death(YLLs), years lived with disability(YLDs) and disability adjusted life years(DALYs) were analyzed to evaluate the burden of malignant neoplasms. Results The total burden of malignant neoplasms in Yantai City in 2012 was 31.05 DALYs per thousand persons.The DALYs per thousand males was 39.12 which was higher than that (22.96) per thousand females.There was a lower DALY rate (25.07 per thousand persons) in urban residents than that (32.03 per thousand persons) in county residents and in district residents(31.12 per thousand persons). Lung cancer, liver cancer, stomach cancer,colon-rectal cancer and breast cancer were the top five cancers with the highest health burden.The DALYs of malignant neoplasms increased with age. Conclusion The burden of malignant neoplasms in Yantai population is heavier than that of the international average level. We should pay more attention to the disease control and prevention pointing at the main caners and population.