JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (8): 16-22.doi: 10.6040/j.issn.1671-7554.0.2014.665

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Prestin expression in outer hair cells of Spag6 gene knockout mice

WANG Jinghan1,2, LI Xiaofei1,3, ZHANG Zhibing4, WANG Haibo1,3, LI Jianfeng2,3   

  1. 1. Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Pathology and Pathophysiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    3. Shandong Provincial Key Laboratory of Otology, Jinan 250021, Shandong, China;
    4. Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia 19104, Pennsylvania, USA
  • Received:2014-10-08 Published:2015-08-10

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Abstract: Objective To detect the prestin expression in sperm associated antigen 6 (Spag6) gene knockout mice and its possible relationship with SPAG6. Methods Through a confocal microscope, the isolated basilar membrane was immunostained for observing the distribution of prestin and SPAG6 in hair cells. In particular, the fluorescence intensity of prestin and the morphological alterations of hair cells were compared among wild type mice (Spag6+/+), heterozygous mice (Spag6+/-) and the corresponding Spag6 gene knockout littermates (Spag6-/-). The expressions of prestin protein and mRNA in mice of three genotypes were measured by Western blotting and real-time Q-PCR. Results SPAG6, which was positive at the cuticular plate and co-localized with prestin at the cell lateral wall, expressed in outer hair cells (OHCs) of both wild type (Spag6+/+) and heterozygous (Spag6+/-) adult mice. Compared with wild type (Spag6+/+) mice, gene knockout mice (Spag6-/-) showed the lower fluorescence intensity of prestin and apparent morphological abnormity of OHCs. The expressions of prestin at protein and mRNA levels in gene knockout mice (Spag6-/-) were significantly decreased as evidenced by Western blotting and real-timeQ-PCR. Conclusion SPAG6 participates in the formation of the cell membrane of OHCs and affects the expression of prestin in OHCs, which implies a possible interaction of these two proteins.

Key words: Sperm associated antigen 6, Gene knockout mice, Prestin, Auditory dysfunction, Outer hair cells

CLC Number: 

  • R764
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