JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (1): 11-16.doi: 10.6040/j.issn.1671-7554.0.2015.305

Previous Articles     Next Articles

Expression of NLRX1 in cochlear hair cells of C57BL/6 mice

YANG Qianqian1,2, SUN Gaoying1,3, CAO Zhixin2,4, YIN Haiyan1,2, WANG Haibo1,3, LI Jianfeng2,3   

  1. 1. Department of Otolaryngology &
    Head and Neck Surgery, Shandong Provincial Hospital Affiliated toShandong University, Jinan 250021, Shandong, China;
    2. Department of Pathology and Pathophysiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    3. Shandong Provincial Key Laboratory of Otology, Jinan 250021, Shandong, China;
    4. Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-03-23 Online:2016-01-11 Published:2016-01-11

PDF (PC)

314

Abstract: Objective To explore the expression of nucleotide-binding domain and leucine-rich-repeat-containing family member X1(NLRX1)in the cochlear hair cells and its dynamic changes during the development and aging of C57BL/6 mice. Methods Basilar membranes of C57BL/6 mice in different age groups, 4-day, 1-month, 3-month and 9-month, were obtained. The location and changes of NLRX1 expression were detected with immunofluorescence, Western blotting and real-time PCR. Results There was no expression of NLRX1 in neonatal mice on day 4 postnatally. The 1-month old mice only expressed NLRX1 in inner hair cells(IHCs), especially in the cilia. The 3-month old mice showed NLRX1 expression in both IHCs and outer hair cells(OHCs). However, the 9-month old mice had markedly lower expression of NLRX1, which exhibited the entire lost expression in OHCs and partial lost expression in IHCs, with some OHCs missing. Conclusion The expression of NLRX1 in C57BL/6 mice cochlear hair cells increases during development, but decreases in the process of aging. It is reasonable to speculate that NLRX1 expression in hair cells may be related to the formation and maintaining of hearing.

Key words: C57BL/6 mice, Hair cells, NLRX1, Immunofluorescence

CLC Number: 

  • R764
[1] Moore CB, Bergstralh DT, Duncan JA, et al. NLRX1 is a regulator of mitochondrial antiviral immunity[J]. Nature, 2008, 451(7178):573-577.
[2] Tattoli I, Carneiro LA, Jéhanno M, et al. NLRX1 is a mitochondrial NOD-like receptor that amplifies NF-kappaB and JNK pathways by inducing reactive oxygen species production[J]. EMBO Rep, 2008, 9(3):293-300.
[3] Lupfer C, Kanneganti TD. Unsolved mysteries in NLR biology[J]. Front Immunol, 2013, 4:285. doi:10.3389/fimmu.2013.00285.
[4] Loeb LA, Wallace DC, Martin GM. The mitochondrial theory of aging and its relationship to reactive oxygen species damage and somatic mtDNA mutations[J]. Proc Natl Acad Sci U S A, 2005, 102(52):18769-18770.
[5] Soares F, Tattoli I, Rahman MA, et al. The mitochondrial protein NLRX1 controls the balance between extrinsic and intrinsic apoptosis[J]. J Biol Chem, 2014, 289(28):19317-19330.
[6] Tadros SF, D'Souza M, Zhu X, et al. Gene expression changes for antioxidants pathways in the mouse cochlea: relations to age-related hearing deficits[J]. PLoS One, 2014, 9(2):90279. doi:10.1371/journal.pone.0090279.ecollection 2014.
[7] Chen H, Tang J. The role of mitochondria in age-related hearing loss[J]. Biogerontology, 2014, 15(1):13-19.
[8] Pearson F, Mann KD, Nedellec R, et al. Childhood infections, but not early life growth, influence hearing in the Newcastle thousand families birth cohort at age 14 years[J]. BMC Ear Nose Throat Disord, 2013, 13:9. doi: 10.1186/1472-6815-13-9. eCollection 2013.
[9] Dong Y, Li M, Liu P, et al. Genes involved in immunity and apoptosis are associated with human presbycusis based on microarray analysis[J]. Acta Otolaryngol, 2014, 134(6):601-608.
[10] Kidd Iii AR, Bao J. Recent advances in the study of age-related hearing loss: a mini-review[J]. Gerontology, 2012, 58(6):490-496.
[11] Lee KY. Pathophysiology of age-related hearing loss(peripheral and central)[J]. Korean J Audiol, 2013, 17(2): 45-49.
[12] O'Neill LA. Innate immunity: squelching anti-viral signalling with NLRX1[J]. Curr Biol, 2008, 18(7):302-304.
[13] Scott I. The role of mitochondria in the mammalian antiviral defense system[J]. Mitochondrion, 2010, 10(4): 316-320.
[14] Schwander M, Kachar B, Muller U. Review series: the cell biology of hearing[J]. J Cell Biol, 2010, 190(1):9-20.
[15] 李丽贤, 杨仕明, 孙建和, 等. 出生后大鼠听毛细胞纤毛发育过程[J]. 中华耳科学杂志, 2009, 7(4):318-323. LI Lixian, YANG Shiming, SUN Jianhe, et al. The development of hair cell stereocilia the postnatal rat Cortis organ[J]. Chinese Journal of Otology, 2009, 7(4):318-323.
[16] Tian C, Kim YJ, Lim HJ, et al. Red ginseng delays age-related hearing and vestibular dysfunction in C57BL/6 mice[J]. Exp Gerontol, 2014, 57:224-232. doi:10.1016/j.exger.2014.06.013. Epub 2014 Jun 19.
[17] Spaulding CC, Walford RL, Effros RB. Calorie restriction inhibits the age-related dysregulation of the cytokines TNF-alpha and IL-6 in C3B10RF1 mice[J]. Mech Ageing Dev, 1997, 93(1-3):87-94.
[18] Yimtae K, Song H, Billings P, et al. Connection between the inner ear and the lymphatic system[J]. Laryngoscope, 2001, 111(9):1631-1635.
[19] Eitas TK, Chou WC, Wen H, et al. The nucleotide-binding leucine-rich repeat(NLR)family member NLRX1 mediates protection against experimental autoimmune encephalomyelitis and represses macrophage/microglia-induced inflammation[J]. J Biol Chem, 2014, 289(7):4173-4179.
[20] 谢鼎华,杨伟炎,韩德民.听力与耳聋基础和临床现代进展[M]. 长沙: 湖南科学技术出版社, 2007: 91-96.
[1] WANG Jinghan, LI Xiaofei, ZHANG Zhibing, WANG Haibo, LI Jianfeng. Prestin expression in outer hair cells of Spag6 gene knockout mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 16-22.
[2] LI Zhuo1, JIANG Huili2, LIU Dianwei2, XIE Jie1, JIANG Yong2. Effects of Wnt3a on the neurogenesis in rats during early brain  injury after subarachnoid hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 15-19.
[3] LI Zi, ZHANG Xuping, JIAO Haimiao, LIU Luqi. Detection of cross-linking degree in artificial aeart valves with amine-specific fluorescent probe [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(12): 1-4.
[4] YANG Zhiying, LIU Xiangchun, GUAN Guangju. Expression changes of methionine sulfoxide reductase B1 in the kidneys of instreptozocin-induced diabetic mice and its relationship with oxidative stress [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(10): 29-34.
[5] ZHOU Hong-xia1, LOU Ning2, WEI Zi-feng1, ZHANG Yu-xin1, MENG Ling-li1, ZHANG Zuo-feng1. Expressions of NF-κB and IL-6 around the hematoma and their correlations
with cerebral edema after intracerebral hemorrhage in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(11): 30-33.
[6] CHEN Meng1, REN Ning2, LIU Wen-jun1, QIN Xiao-min1, LI Jin-song1. Screening of molecular markers for epidermal stem cells in mice by the double immunofluorescence labeling technique [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 65-69.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd