JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (1): 11-16.doi: 10.6040/j.issn.1671-7554.0.2015.305

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Expression of NLRX1 in cochlear hair cells of C57BL/6 mice

YANG Qianqian1,2, SUN Gaoying1,3, CAO Zhixin2,4, YIN Haiyan1,2, WANG Haibo1,3, LI Jianfeng2,3   

  1. 1. Department of Otolaryngology &
    Head and Neck Surgery, Shandong Provincial Hospital Affiliated toShandong University, Jinan 250021, Shandong, China;
    2. Department of Pathology and Pathophysiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    3. Shandong Provincial Key Laboratory of Otology, Jinan 250021, Shandong, China;
    4. Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-03-23 Online:2016-01-11 Published:2016-01-11

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Abstract: Objective To explore the expression of nucleotide-binding domain and leucine-rich-repeat-containing family member X1(NLRX1)in the cochlear hair cells and its dynamic changes during the development and aging of C57BL/6 mice. Methods Basilar membranes of C57BL/6 mice in different age groups, 4-day, 1-month, 3-month and 9-month, were obtained. The location and changes of NLRX1 expression were detected with immunofluorescence, Western blotting and real-time PCR. Results There was no expression of NLRX1 in neonatal mice on day 4 postnatally. The 1-month old mice only expressed NLRX1 in inner hair cells(IHCs), especially in the cilia. The 3-month old mice showed NLRX1 expression in both IHCs and outer hair cells(OHCs). However, the 9-month old mice had markedly lower expression of NLRX1, which exhibited the entire lost expression in OHCs and partial lost expression in IHCs, with some OHCs missing. Conclusion The expression of NLRX1 in C57BL/6 mice cochlear hair cells increases during development, but decreases in the process of aging. It is reasonable to speculate that NLRX1 expression in hair cells may be related to the formation and maintaining of hearing.

Key words: C57BL/6 mice, Hair cells, NLRX1, Immunofluorescence

CLC Number: 

  • R764
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