Table of Content

  

10 September 2015
Volume 53 Issue 9

Castration-resistant prostate cancer: current understanding of mechanisms and emerging novel agents

HAN Bo, QI Mei, TAN Weiwei, YANG Muyi

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  1-7.  doi:10.6040/j.issn.1671-7554.2.2015.002

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Castration-resistant prostate cancer (CRPC) has an extremely poor prognosis and remains a significant clinical challenge as the underlying mechanisms are largely unknown. Recently, a broad range of novel therapeutic drugs has emerged for CRPC treatment, including androgen synthesis inhibitors (abiraterone), androgen receptor (AR) inhibitors (enzalutamide), immunotherapy (sipuleucel-T), radiopharmaceuticals (Radium-223) and chemotherapeutic agents(cabazitaxel). Therefore, clinical urologists have more choices for the treatment of CRPC patients and the strategies of the systematic therapy have undergone great changes. In this review, we briefly overviewed the current molecular understanding of CRPC and highlighted the recently approved and emerging therapeutics for patients with CRPC.

Effects of factors secreted by mouse embryonic stem cells on prostate cancer cells in vitro

ZHAI Hongyun, ZHANG Denglu, WANG Guangjie, KONG Feng, CHENG Guanghui, ZHAO Shengtian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  8-12.  doi:10.6040/j.issn.1671-7554.0.2015.219

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Objective To study the effects of factors secreted by mouse embryonic stem cells (ESC) on the biological characteristics of prostate cancer cells RM-1 and to explore the mechanism. Methods A non-contact co-culture system was established with Transwell chambers in vitro. The chambers connecting ESC combined with mouse embryonic fibroblasts (MEF) and RM-1 cells served as experimental group, while the chambers connecting MEF and RM-1 cells were negative controls. After cells were co-cultured for 72 h, the proliferation of RM-1 cells in the two groups were measured. Differences of RM-1 cells in cell morphology, cell cycle, apoptotic level, migration and invasion ability between the two groups were observed. Results The RM-1 cells in experimental group were loosely arranged with less cell division and more cell death, and some cells showed signs of apoptosis. The RM-1 cells in experimental group proliferated more slowly (P<0.01), with cell cycle arrest between phase G₁ and S in addition with increased apoptosis, especially late stage apoptosis (both P<0.01). The RM-1 cells in experimental group showed weaker migration and invasion ability (both P<0.05). Conclusion The factors secreted by ESC exhibited anti-tumor effects. They inhibited RM-1 cell proliferation by arresting cell cycle, increasing cell apoptosis, and weakening cell migration and invasion ability.

Effects of nitidine chloride on the proliferation and apoptosis of prostate cancer PC-3 cells

CHENG Xiangyu, XING Rui, XING Zhaoquan, GUO Zhaoxin, GUO Xiaoyu, SU Jing, MENG Liwei, LIU Zhaoxu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  13-18.  doi:10.6040/j.issn.1671-7554.0.2015.281

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Objective To evaluate the effects of nitidine chloride on the proliferation and apoptosis of prostate cancer cells PC-3 in vitro. Methods MTT, scratch migration and Transwell were used to examine the changes in the proliferation and invasions of human prostate cancer cell PC-3 after nitidine chloride treatment. Flow cytometry was used to examine the changes in cell apoptosis of PC-3. Immunoblot analysis was adopted to detect the expression of AKT/mTOR, Bcell-associated x protein (Bax) and Bcell lymphoma-2 (Bcl-2). A specific PI3K inhibitor, LY294002, was used to evaluate the influence of prostate cancer cells by inhibiting AKT pathway. Results Nitidine chloride inhibited the proliferation and invasion of PC-3 cells in a dose-dependent manner (P<0.01). Nitidine chloride inhibited AKT and mTOR pathways phosphorylation, accompanied by up-regulation of Bax, and down-regulation of Bcl-2. Nitidine chloride significantly increased the ratio of Bax/Bcl-2 (P<0.01), to induce prostate cancer cells apoptosis. Furthermore, combined use of LY294002 inhibited AKT pathway, which could enhance the anti-proliferation and anti-invasion effectsof nitidine chloride. Conclusion Nitidine chloride can suppress the proliferation, migration, invasion and induce the apoptosis of prostate cancer cells in vitro. Nitidine chloride also plays a role in anti-cancer via inhibiting AKT/mTOR pathway phosphorylation, which implies that nitidine chloride may be a promising therapeutic drug for prostate cancer.

Effects of chemical composition of urinary stones on their acoustic shadowing

LIU Qing, SHANG Mengmeng, SUN Xiao, LI Jie, YANG Jie, SHI Dandan, NING Song

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  19-23.  doi:10.6040/j.issn.1671-7554.0.2014.931

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Objective To quantitatively analyze the association between chemical composition of urinary stones and acoustic shadowing. Methods Calcium oxalate monohydrate, hydroxyapatite, cystine, and uric acid stone phantoms were prepared and embedded in porcine kidneys for ultrasound and CT scanning. The contrast-to-noise ratio (C/N) of acoustic shadowing and Hounsfield unit were recorded. All data obtained were analyzed statistically. Results All stones demonstrated high acoustic echo with shadowing. Of all stone phantoms, C/N ratio was 1.01-3.74, 2.17 in average. The hydrocyapatite stones displayed the strongest C/N ratio of acoustic shadowing and Hounsfield unit, followed by calcium oxalate monohydrate, cystine and uric acid stones (all P<0.001). The C/N ratio was significantly correlated with the Hounsfield unit (r=0.942,P<0.001) for all stone phantoms. Conclusion Stones with different chemical compositions have different acoustic shadowing, but the clarity of acoustic shadowing has the same trend with the Hounsfield unit, and acoustic shadowing can be used to predict the chemical compositions.

Role of CXCL16 in mice with adriamycin induced nephropathy and the protective effects of simvastatin

WANG Cong, SUN Shuzhen, ZHEN Junhui, LI Qian, XU Yihuai

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  24-29.  doi:10.6040/j.issn.1671-7554.0.2014.908

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Objective To investigate the roles of chemokine ligand 16 (CXCL16) and oxidized low density lipoprotein (ox-LDL) in mice model with adriamycin induced nephropathy and their relationship with the renal tissue morphology, in order to explore the renal protective effects of simvastatin on adriamycin nephropathy. Methods We divided the mice into the following three groups: control group (n=5), adriamycin nephropathy group (n=5) and simvastatin treated adriamycin nephropathy group (n=5). Each group was treated with corresponding treatment respectively. 5 weeks after injection of adriamycin, the blood and 24 h urine were collected for the detection of general biochemical parameters and serum ox-LDL. After blood and 24 h urine collected, the mice were weighed and sacrificed with kidneys harvested. The renal morphology was observed by light microscopy and transmission electron microscopy, the expressions of CXCL16 and ox-LDL in podocytes were detected by double immunofluorescence techniques. Results Compared with the control group, the levels of TC and ox-LDL in the adriamycin nephropathy and simvastatin treated adriamycin nephropathy were significantly higher (P<0.05), the expressions of CXCL16 and ox-LDL in renal tissue were significantly increased (P<0.05). Compared with the adriamycin nephropathy group, the expressions of renal CXCL16 and ox-LDL in simvastatin treated adriamycin nephropathy group were markedly reduced (P<0.05), but there were no significant differences found in serum TC and ox-LDL between adriamycin nephropathy and simvastatin treated adriamycin nephropathy group. Conclusion Simvastatin has a protective effect on kidney in mice with adriamycin nephropathy. The mechanism might be related to the decreasing expression of CXCL16 in glomerular podocytes, followed by the decreasing endocytosis of ox-LDL in podocytes.

Changes on the expressions of alpha1-adrenergic receptor and nerve growth factor and their effects on urethral function in diabetic rats

ZHANG Dongqing, WANG Yong, CHEN Shouzhen, ZHU Yaofeng, SHI Benkang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  30-34.  doi:10.6040/j.issn.1671-7554.0.2015.581

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Objective To explore the changes on the expressions of α1-adrenergic receptor and nerve growth factor (NGF) and theirs effects on the urethral function in diabetic rats. Methods A total of 20 female Wistar rats were randomly divided into 2 groups. Urethral function was examined by detecting the bladder pressure and urethral perfusion pressure (UPP). The expressions of α1-adrenergic receptor and NGF in the urethra were measured with RT-qPCR and ELISA. The expressions of proNGF, P75^NTR and Sortilinin in the urethra were evaluated with Western blotting. Results The lowest urethral pressure (UPP nadir) during urethral relaxation was higher in diabetic rats. The UPP nadir and baseline UPP in diabetic rats was significantly decreased by intravenous administration of α1-adrenoceptor antagonist (tamsulosin). The α1a and α1d adrenergic receptor in the urethra of the diabetic group was significantly increased as detected with RT-qPCR and Western blotting (P<0.05). RT-qPCR and ELISA studies showed a significant decrease of NGF, and Western blotting indicated a significant increase of proNGF (P<0.05). There was a statistical decrease of P75^NTR in the urethra of diabetic rats (P<0.05), but no significant difference concerning Sortilin between the two groups (P>0.05). Conclusion The increase in the expression of α1-adrenoceptor and changes of NGF/ProNGF pathway in diabetic rats is a possible mechanism of diabetic urethral dysfunction.

Construction of the minireplicon of enterovirus 71

MA Yingwei, SUN Lele, LI Jing, QIAO Qiao, ZHUANG Zhichao, ZHAO Li, YU Xuejie, WANG Zhiyu, WEN Hongling, SUN Yanlin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  35-40.  doi:10.6040/j.issn.1671-7554.0.2015.096

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Objective To construct the minireplicon of non-structural protein and 3C protease of EV71 and study the replication and expression of the minireplicon. Methods The genes of the enhanced green fluorescent protein(EGFP), amplified by PCR, and the genes of non-structural protein and 3C protease were inserted into pMD19-T vector through specific restriction sites and ligase. The expression and replication of the minireplicon were detected by fluorescence microscope and checked by real-time PCR respectively. Results Specific green fluorescence was detected after in vitro transcription and transfection of the plasmid containing the genes of EV71 non-structural proteins and the 3C protease, and there were significant differences in the intensity of the fluorescence among different groups. The content of the RNA remained same between 12 h to 72 h after transfection. Conclusion The abilities of transcriptions of the full-length non-structural protein and the 3C protease were different. The replication of the minireplicon could not be detected in RD cells, which suggested the importance of the structural protein to the replication of the virus.

Protective effect of grape seed proanthocyanidins in mouse fatty liver models suffering ischemia-reperfusion injury

LI Shaowei, LIU Zongzheng, LIU Chunxia, ZHANG Yanru, ZHOU Huanmin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  41-46.  doi:10.6040/j.issn.1671-7554.0.2014.716

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Objective To explore the protective effects of grape seed proanthocyanidins (GSP) on liver ischemia-reperfusion (IR) in mouse fatty liver models and the possible mechanism. Methods Thirty C57BL/6 mice were randomly divided into 3 groups: sham-operated group (SO group), IR group (mice with steatotic liver treated with distilled water by gavage just before ischemia reperfusion) and GSP group (mice with steatotic liver gavaged with 500 mg/kg GSP). The mice were then sacrificed 6h after reperfusion, and serum and liver samples were collected. The ALT and AST levels in serum were determined and histiological damage on the liver was evaluated. The infiltration of F4/80 positive macrophage cells was detected using immunohistochemistry. The mRNA expressions of TNF-α, IL-6, iNOS, and INF-γ were assayed using real-time reverse transcription PCR. The expression of p38 protein was detected using Western blotting. Results Compared with the IR group, mice in the GSP group exhibited lower levels of serum ALT and AST (P<0.05), and milder histological damage. Compared with the IR group, mice in the GSP group had reduced infiltration of macrophages and lower mRNA expressions of TNF-α, IL-6, iNOS and INF-γ in the liver tissues (P<0.05). The p38 expressions in the three groups had no difference, but the phosphorylated p38 (p-p38) expression in the GSP group was statistically higher than in the other two groups. Conclusion GSP can alleviate steatotic liver IR injury by inhibiting oxidant stress and inflammatory response induced by reperfusion.

Establishment of rat models of rheumatoid arthritis induced by Borrelia burgdorferi

WANG Weiyan, YANG Qingrui, SHI Jianmin, ZHAO Lulian, ZHANG Yuanchao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  47-52.  doi:10.6040/j.issn.1671-7554.0.2015.019

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Objective To establish rat models of rheumatoid arthritis by primary intradermal and secondary intraperitoneal injections of whole spirochaeta protein of Borrelia burgdorferi (B.b), and to explore the pathogenesis of the disease. Methods A total of 54 Lewis rats were randomly divided into low-dose B.b group, medium-dose B.b group, high-dose B.b group, complete Freunds adjuvant group, incomplete Freunds adjuvant group and PBS group, with 9 rats in each group. The degree of pathogenicity and pathological characteristics were assayed with arthritis index and histiopathological techniques. Serum cytokines were measured with enzyme linked immunosorbent assay (ELISA). Results The incidence of arthritis in high-dose B.b group was 66.7%. Histopathological tests showed typical arthritis pathology, including synovial proliferation, articular cartilage and bone erosion. The arthritis index, histological score and cytokine expressions of the high-dose B.b group had no statistical difference compared with those of the complete Freunds adjuvant group (P>0.05), but they were significantly higher than those of the PBS group (P<0.05). Conclusion Arthritis induced by whole spirochaeta protein of B.b mirrors human rheumatoid arthritis and is one of the ideal animal models for studying rheumatoid arthritis.

Over expression of lipin1 attenuates diabetic peripheral neuropathy in rats

XU Yi, ZHUANG Xianghua, LIU Yuantao, SUN Aili, LI Xiaobo, JIANG Dongqing, ZHANG Liang, XU Min, YAN Heng, CHEN Shihong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  53-57.  doi:10.6040/j.issn.1671-7554.0.2015.296

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Objective To investigate the effects of lipin1 on diabetic peripheral neuropathy in rats. Methods Type 2 diabetes mellitus was induced in male Wistar rats which were fed with high fatty and high sugar diet for 8 weeks, followed by streptozotocin (STZ) injection intraperitoneally. And then the models were randomly divided into Lipin1 high expression (LV-Lipin1) group and empty vector (LV-control) group. Another 12 age-matched non-diabetic rats served as controls. Rats in LV-Lipin1 group were transfected with lentiviral vector expressing rat lipin1; rats in LV-control group were transfected with lentiviral empty vector. After two months, fasting blood glucose, body weight and the motor nerve conduction velocity (MNCV) were measured. Histopathological changes of sciatic nerve were analyzed with light microscope and electron microscope; Lipin1 expression was evaluated with immunohistochemistry. Results Two months after diabetes was induced, expression of Lipin1 in sciatic nerves was significantly decreased in the LV-control group compared with control group. The nerve fiber diameter, nerve fiber area and nerve fiber density in LV-control group were also decreased compared with control group. All of the above changes were significantly attenuated in LV-Lipin1 group. Motor nerve conduction velocity (MNCV) of sciatic nerve in LV-Lipin-1 group was significantly improved compared with LV-control group (P<0.05). Conclusion Expression of Lipin1 in sciatic nerve decreases in diabetic peripheral neuropathy rats. Upregulation of Lipin1 expression significantly improves the MNCV and nerve pathological morphology.

Role and possible mechanism of autophagy in ubenimex-induced cell death of renal cell carcinoma

HUANG Zhilong, LIU Shuai, WANG Jianwei, HAN Liping, WANG Xiaoqing, LI Xin, BI Dongbin, XIE Fang, NIU Zhihong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  58-64.  doi:10.6040/j.issn.1671-7554.0.2015.415

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Objective To determine the effect of ubenimex on the proliferation of renal cell carcinoma cells and the possible mechanism. Methods WST-8 cell proliferation assay and cell growth curve were used to detect the proliferation of renal cell carcinoma cells. LDH cytotoxicity assay was used to detect the cell death of the renal cell carcinoma. Alanine-p-nitroanilido was used to detect the activity and expression of APN induced by ubenimex in renal cell carcinoma cell lines. Western blotting and immunohistochemical staining were used to detect the expression of APN/CD13 in tissues of normal kidney and renal cell carcinoma. Results Ubenimex could not reduce the express of APN in renal cell carcinoma but inhibit its activity. Ubenimex could inhibit the proliferation and activity of renal cell carcinoma cells, and induce autophagic cell death. Conclusion Ubenimex inhibits the proliferation of renal cell carcinoma cells and promotes levels of cell death in renal cell carcinoma cells via an autophagic mechanism.

Comparison of nephron-sparing surgery and radical nephrectomy for T1b renal cell carcinoma: a Meta-analysis

SU Jing, XUE Jiaomei, SUN Feifei, GUO Zhaoxin, CHENG Xiangyu, MENG Liwei, LIU Zhaoxu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  65-70.  doi:10.6040/j.issn.1671-7554.0.2014.830

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Objective To compare the outcomes of nephron-sparing surgery (NSS) and radical nephrectomy (RN) in treating T1b renal cell carcinoma (RCC). Methods The following databases including Pubmed, Elsevier ScienceDirect, Web of Science, Co-chrane library, CNKI, CBM, VIP and WanFang were searched electronically from the date of their establishment to Sept. 2014 to collect cohort studies on NSS versus RN in the treatment of T1b RCC, and the references of those studies were manually retrieved. After the studies were screened according to the inclusion and exclusion criteria, data were extracted and assessed, and Meta-analysis was performed using the Stata 12.0 software. Results A total of 11 studies with 23,939 patients were included. The Results of Meta-analyses showed that, RN and NSS had no significant difference in 5-year[RR=1.05, 95% CI(0.98, 1.13)], 7-year [RR=1.02, 95% CI (0.94, 1.10)], and 10-year [RR=1.30, 95% CI (0.96, 1.77)] overall survival (OS). Nevertheless, NSS had higher 5-year cancer specific survival (CSS) [RR=1.11, 95% CI (1.06, 1.16)], lower 5-year cancer specific mortality (CSM) [RR=0.70, 95% CI (0.55, 0.90)] and lower metastasis rate [RR=0.38, 95% CI(0.22, 0.66)]. Conclusion For the patients who are included in the study, NSS and RN is comparable in 5-year OS, 7-year OS, 10-year OS; NSS is superior to RN in 5-year CSS, 5-year CSM and metastasis rate.

Serum TK1 for the diagnosis of cancer in Chinese patients: a Meta-analysis

LIU Jingsheng, LIU Guihua, DU Kun, ZHAO Qian, WENG Shaobo, ZHAO Xueying, ZHANG Xizhuang, JIN Xunbo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  71-79.  doi:10.6040/j.issn.1671-7554.0.2015.514

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Objective To evaluate significance of thymidine kinase 1 (TK1) detection in the diagnosis of malignant tumors in Chinese patients. Methods Clinical trials on TK1 detection published during Jan. 2008 to Mar 2015 were searched in databases of CNKI and Wan Fang. Revman 5.2 and Meta Disc 1.4 were used to perform the Meta-analysis.Results A total of 415 relevant articles were retrieved and 45 trials with 2,976 cancer patients (all diagnosed by the gold standard) were involved. Meta-analyses showed that the heterogeneity among studies was high, and the pooled Results suggested a moderate diagnostic efficacy. The sensitivities for lung cancer, breast cancer, gynecological cancer, prostatic cancer, gastric cancer, intestinal cancer and esophagus cancer were 0.508, 0.638, 0.524, 0.656, 0.583, 0.546, and 0.681, respectively. The specificities were 0.973, 0.932, 0.940, 0.925, 0.941, 0.947, and 0.967. Conclusion TK1 detection may have certain value in the early diagnosis and screening of malignant cancers, but it can not be serveed as an independent reference test. More high quality trials are required to confirm the significance of TK1 detection.

Different expressions of novel microRNAs of iPSCs from Alport syndrome

CHEN Wenbiao, YU Xiangqi, DAI Yong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  80-85.  doi:10.6040/j.issn.1671-7554.0.2015.307

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Objective To investigate the expression profile of iPSCs of novel microRNAs between patients with Alport syndrome (AS) and normal controls (NC), and to analyze the target genes. Methods The expression profile of novel microRNAs was acquired from previously induced iPSCs, using high-throughput sequencing platform. After that, TargetScan software was adopted to predict target genes, which were then compared with reference genes, and the significantly encriched GO items and KEGG pathways were selected. Results A total of 49 differently expressed novel microRNAs were selected, 33 of which were up-regulated and 16 were down-regulated. GO enrichment analysis indicated that the target genes mainly enriched in biological regulation and metabolism; cell component and signal transduction; enzymic catalytic reaction; transporter activity. KEGG pathway analysis showed that the target genes mainly participated in metabolic pathways, purine metabolism, and cancer transcriptional regulation. Conclusion Novel microRNAs of iPSCs from AS and from NC are differently expressed, and the target genes mainly take part in molecular function, cellular component, and biological activities. Those differently expressed novel microRNAs and target genes may play an important role in the pathogenesis of AS.

Clinical outcome of unilateral external fixator combined with closed negative pressure drainage technology in the treatment of tibiofibula Gustilo Ⅲ type fracture

DONG Wuping

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  86-89.  doi:10.6040/j.issn.1671-7554.1.2015.001

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Objective To explore the clinical outcome of unilateral external fixator combined with vacuum sealing drainage (VSD) technology in the treatment of tibiofibula Gustilo Ⅲ type fracture. Methods A total of 57 patients who were treated with unilateral external fixator combined with VSD from July 2010 to April 2015 were taken as observation group, and 59 patients who were treated with open reduction and plate fixation from July 2006 to June 2010 were selected as control group. The average osseous healing time, length of hospital staying, intraoperative blood loss and postoperative complications of the two groups were observed. All the patients were followed up for half a year. Results The average osseous healing time[(180.65±30.36)d], length of hospital staying[(27.84±3.87)d], intraoperative blood loss[(393.56±12.09)mL] in observation group were significantly lower than those of control group[(340.00±40.51)d, (46.27±4.84)d and (545.36±50.40)mL, all P<0.05]. The osteomyelitis rate(1.75%), superficial infection rate(5.26%) and malunion rate(0.00%) in observation group were respectively lower than those in control group (11.86%, 21.05%, 8.47%, all P<0.05). The rates of good and excellent/good in observation group were 80.70% and 91.23%, which were significantly higher than those in control group (61.02% and 77.97%, all P<0.05). Conclusion Unilateral external fixator combined with VSD is a good way to treat tibiofibula Gustilo Ⅲ type fracture, which is beneficial to fracture healing and can reduce the length of hospital stay and complications.

Relationship between personality and psychological distress in nurses of psychiatric department: mediating effect of resilience

LIU Chunqin, LI Huafang, TIAN Xiaohong, ZOU Guiyuan, LI Ping

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2015, 53(9):  90-94.  doi:10.6040/j.issn.1671-7554.0.2014.717

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Objective To explore the mediating effect of resilience between psychological distress and personality in nurses of psychiatric department. Methods A total of 192 nurses from one mental health center in Shandong Province were assessed with Big Five Inventory (BFI), Connor-Davidson Resilience Scale (CD-RISC 10) and Kessler Psychological Distress Scale (K10). Results The K10 score was 20.66±5.91; 80.7% of the nurses had psychological distress (K10 score≥16). The K10 score was positively correlated with neuroticism score (P<0.01), and negatively correlated with extroversion and conscientiousness and resilience scores (P<0.01). Resilience score was positively correlated with extroversion and conscientiousness scores (P<0.01), and negatively correlated with neuroticism score (P<0.01). Resilience partially mediated the relationship between psychological distress and neurotic personality, and the mediating effect accounted for 25.4% of the total effects. Conclusion Neurotic personality and resilience have a great impact on the psychological distress in nurses of psychiatric department; resilience is a partial mediator in neuroticism affecting psychological distress.