JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 30-34.doi: 10.6040/j.issn.1671-7554.0.2015.581

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Changes on the expressions of alpha1-adrenergic receptor and nerve growth factor and their effects on urethral function in diabetic rats

ZHANG Dongqing, WANG Yong, CHEN Shouzhen, ZHU Yaofeng, SHI Benkang   

  1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-06-17 Online:2015-09-10 Published:2015-09-10
  • Contact: 史本康。E-mail:benkangshi@126.com E-mail:benkangshi@126.com

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Abstract: Objective To explore the changes on the expressions of α1-adrenergic receptor and nerve growth factor (NGF) and theirs effects on the urethral function in diabetic rats. Methods A total of 20 female Wistar rats were randomly divided into 2 groups. Urethral function was examined by detecting the bladder pressure and urethral perfusion pressure (UPP). The expressions of α1-adrenergic receptor and NGF in the urethra were measured with RT-qPCR and ELISA. The expressions of proNGF, P75NTR and Sortilinin in the urethra were evaluated with Western blotting. Results The lowest urethral pressure (UPP nadir) during urethral relaxation was higher in diabetic rats. The UPP nadir and baseline UPP in diabetic rats was significantly decreased by intravenous administration of α1-adrenoceptor antagonist (tamsulosin). The α1a and α1d adrenergic receptor in the urethra of the diabetic group was significantly increased as detected with RT-qPCR and Western blotting (P<0.05). RT-qPCR and ELISA studies showed a significant decrease of NGF, and Western blotting indicated a significant increase of proNGF (P<0.05). There was a statistical decrease of P75NTR in the urethra of diabetic rats (P<0.05), but no significant difference concerning Sortilin between the two groups (P>0.05). Conclusion The increase in the expression of α1-adrenoceptor and changes of NGF/ProNGF pathway in diabetic rats is a possible mechanism of diabetic urethral dysfunction.

Key words: α1-adrenoceptor, Rats, Urethra, Diabetes mellitus, Nerve growth factor

CLC Number: 

  • R695
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