JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (11): 15-21.doi: 10.6040/j.issn.1671-7554.0.2017.144

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Urethral reconstruction with autologous tubular granulation tissues: functional and histological evaluation in male rabbits

JIANG Shiwei, YAN Lei, TANG Yueqing, REN Juchao, ZANG Yuanwei, ZHANG Yongzhen, GU Gangli, XU Zhonghua   

  1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2017-02-16 Online:2017-11-10 Published:2017-11-10

Abstract: Objective To evaluate the feasibility by using autologous tubular granulation tissues for urethral reconstruction. Methods A total of 18 male rabbits were divided into the experimental group(n=9)and control group(n=9). A pendulous urethral segment of 1.0 cm was excised in all rabbits. In the experimental group, urethroplasty was performed with autologous tubular granulation tissues. In the control group, re-anastomosis was performed. Serial urethrography, general observation, histological detection and Western blotting were performed 1 month, 2 and 6 months after operation to dynamically evaluate patency degree and tissue regeneration. Results Histological and transmission electron microscopy of the autologous granulation tissues showed densely-arranged collagens and myofibroblasts. Urethrography showed the neo-urethra in the experimental group remained wide patent at each time point, while strictures developed in the control group. Histologically, layers of urothelium surrounded by increasingly organized smooth muscles were observed. Western blotting showed expressions of pan-cytokeratin AE1/AE3 and α-smooth muscle actin increased gradually with time, which reached similar level with normal urethra 6 months postoperatively. Conclusion Autologous tubular granulation tissues grown subcutaneously can be used to repair urethral defect of 1.0 cm in male rabbits.

Key words: Urethral reconstruction, Autologous graft, Urethral stricture, Granulation tissue

CLC Number: 

  • R695
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