JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (6): 7-11.doi: 10.6040/j.issn.1671-7554.0.2015.341

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Study of mouse alveolar bone defect reparation by dialdehyde bletilla striata glucomanna / hydroxypropyl chitosan / nano-nacre powder composited scaffolds

CHEN Yihui1, LIU Xuewei1, HOU Xuhao2, SUN Qinfeng1   

  1. 1. Department of Periodontics, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, Shandong, China;
    2. School of Pharmacy, Shandong University of Chinese Traditional Medicine, Jinan 250355, Shandong, China
  • Received:2015-04-01 Online:2016-06-20 Published:2016-06-20

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Abstract: Objective To observe the osteogenesis and degradation of dialdehyde letilla striata glucomanna(DBsGM)/hydroxypropyl chitosan(HPCS)/nano-nacre powder(NNP)composited scaffolds in rat alveolar bone defect regeneration. Methods A total of 32 male Wistar rats with bilateral mandibular molar area box defects were randomly divided into 4 groups according to the disposal time(week 2, 4, 6, 8), with 8 animals in each group. The DBsGM/HPCS/NNP composited scaffolds were implanted in the experimental side, while nothing was implanted in the control side. The bone defect repair effect was evaluated with hematoxylin-eosin staining and histologic measurement. Results As time extended, the percentage of new bone area increased gradually, and there was significant difference between different time points(P<0.05). At the same time point, the experimental group was superior to the control group in gross observation and histological observation(P<0.05). The implanted materials gradually degraded with time. During week 6 to 8, the implanted materials completely degraded. Conclusion DBsGM/HPCS/NNP composited scaffolds can promote rat alveolar bone defect repair and regeneration.

Key words: Tissue engineering, Periodontal tissue regeneration, Nano-nacre powder, Bletilla striata glucomannan, Periodontal defects in rats

CLC Number: 

  • R781
[1] 刘学蔚, 侯绪浩, 陈怡憓, 等. 正交优化白及多糖复合支架材料的实验研究[J]. 山东大学学报(医学版), 2014, 52(3):40-44. LIU Xuewei, HOU Xuhao, CHEN Yihui, et al. Experimental study of the bletilla striata glucomanan composited scaffolds by orthogonal experiment [J]. Journal of Shandong University(Health Sciences), 2014, 52(3):40-44.
[2] Liao F, Chen Y, Li Z, et al. A novel bioactive three-dimensional beta-tricalcium phosphate /chitosan scaffold for periodontal tissue engineering[J]. J Mater Sci Mater Med, 2010, 21(2):489-496.
[3] Yu G, Fan Y. Preparation of poly(D, L-lactic acid)scaffolds using alginate particles [J]. J Biomater Sci Polym Ed, 2008, 19(1): 87-98.
[4] Wang Z, Li M, Yu B, et al. Nanocalcium-deficient hydroxyapatite-poly(e-caprolactone)-polyethylene glycol-poly(e-caprolactone)composite scaffolds[J]. Int J Nanomedicine, 2012, 7:3123-3131.
[5] 陈建庭, 汤勇智, 张建刚, 等. 纳米珍珠层人工骨的制备[J]. 南方医科大学学报, 2008, 28(12):2171-2173. CHEN Jianting, TANG Yongzhi, ZHANG Jiangang, et al. Preparation of nano-nacre artificial bone[J]. Journal of Southern Medical University, 2008, 28(12):2171-2173.
[6] 彭锐, 郑启新, 郝杰. 明胶/白及胶载药多孔材料的体外抑菌试验[J]. 中国中医骨伤科杂志, 2006, 14(3):36-37. PENG Rui, ZHENG Qixin, HAO Jie, et al. Study on antibacterial action of a porous biomaterial(gelatin-bletilla hyacinthine gum/Chinese medicine)[J]. Chinese J Trad Med Traum & Orthop, 2006, 14(3):36-37.
[7] 庞永志, 胡温庭, 彭凤梅. 复方白及胶/珍珠层粉修复兔牙槽骨缺损的实验研究[J]. 山东大学学报(医学版), 2013, 51(1):37-41. PANG Yongzhi, HU Wenting, PENG Fengmei. Experimental study of bletilla glucomannan /nacre powder composites repairing the alveolar bone defects in rabbits[J]. Journal of Shandong University(Health Sciences), 2013, 51(1):37-41.
[8] Lamghari M, Berland S, Laurent A, et al. Bone reactions to nacre injected percutaneously into the vertebrae of sheep [J]. Biomaterials, 2001, 22(6):555-562.
[9] 张卫明, 马士宏, 顾龚平, 等. 白及多糖胶皮肤毒理学安全性评价研究[J]. 中国野生植物资源, 2003, 22(5):59-61.
[10] Kim YW, Kim JJ, Kim YH, et al. Effects of organic matrix proteins on the interfacial structure at the bone2biocompatible nacre interface in vitro [J]. Biomaterials, 2002, 23(9):2089-2096.
[11] Berland S, Delattre O, Borzeix S, et al. Related Articles, Nacre/bone interface changes in durable nacre endosseous implants in sheep[J]. Biomaterials, 2005, 26(15): 2767-2773.
[12] Du Y, Zhao Y, Dai S, et al. Preparation of water-soluble chitosan from shrimp shell and its antibacterial activity[J]. Innovative Food Science & Emerging Technologies, 2009, 10(1):103-107.
[13] Jiang F, Li W, Huang Y, et al. Antioxidant, antityrosinase and antitumor activity comparison: the potential utilization of fibrous root part of Bletilla striata(Thunb.)Reichb.f. [J]. PLoS One, 2013, 8(2):e58004.
[14] 陈德利, 施伟民, 徐倩, 等. 中药白及促进角质形成细胞的游走[J].中华皮肤科杂志, 1999, 32(3):161-162. CHEN Deli, SHI Weimin, XU Qian, et al. Rhizoma Blelillae promotes the migration of keratinocyte[J].Chin J Dermatol, 1999, 32(3):161-162.
[15] 王刚, 常明泉, 杨光义, 等. 白及多糖对氧化损伤的人角质形成细胞JAK/STAT信号通路的影响[J]. 医药导报, 2012, 31(6):701-705.
[16] 孙仁山, 陈晓红, 程天民, 等. 白及对大鼠创面愈合几个要素的影响[J]. 中国临床康复, 2004, 7(29):3927-3929. SUN Renshan, CHEN Xiaohong, CHENG Tianmin, et al. Some factors of bletilla striata in wound healing[J]. Chinese Journal of Clinical Rehabilitation, 2004, 7(29):3927-3929.
[17] Wang C, Sun J, Luo Y, et al. A polysaccharide isolated from the medicinal herb Bletilla striatainduces endothelial cells proliferation and vascular endothelial growth factor expression in vitro[J]. Biotechnol Lett, 2006, 28(8):539-543.
[18] 吴一民, 孙炜. 珍珠层人工骨促进成骨细胞的体外成骨能力[J]. 中国矫形外科杂志, 2003, 11(3):74-76. WU Yimin, SUN Wei. Osteogenic ability of osteoblast promoted by nacre composite artificial bone in vitro[J].Orthop J Chin, 2003, 11(3):74-76.
[19] Asvanund P, Chunhabundit P, Suddhasthira T. Potential induction of bone regeneration by nacre: an in vitro study[J]. Implant Dent, 2011, 20(1):32-39.
[20] Lopez E, Vidal B, Berland S, et al. Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro[J]. Tissue Cell, 1992, 24(5):667-679.
[21] 杨春露, 陈建庭, 金大地, 等. 珍珠层水溶性提取物对人骨髓基质细胞成骨性分化的诱导作用[J]. 中国临床解剖学杂志, 2007, 25(2):190-193. YANG Chunlu, CHEN Jianting, JIN Dadi, et al.The bone induction effect of water soluble matrix from nacre power on human marrow stroma cells[J]. Chinese Journal of Clinical Anatomy, 2007, 25(2):190-193.
[22] 王建钧, 陈建庭, 杨春露. 珍珠层水溶性基质对兔骨髓基质干细胞BMP-2 和Cbfα1 基因表达的影响[J]. 南方医科大学学报, 2007, 27(12):1838-1840. WANG Jianjun, CHEN Jianting, YANG Chunlu. Effects of soluble matrix of nacreon bone morphogenetic protein-2 and Cbfa1 gene expressions in rabbit marrow mesenchymal stem cells[J]. J South Med Univ,2007, 27(12):1838-1840.
[23] Lamghari M, Huet H, Laurent A, et al. A model for evaluating injectable bone replacements in the vertebrae of sheep: radiological and histological study[J]. Biomaterials, 1999, 20(22):2107-2114.
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