JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 24-29.doi: 10.6040/j.issn.1671-7554.0.2014.908

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Role of CXCL16 in mice with adriamycin induced nephropathy and the protective effects of simvastatin

WANG Cong1, SUN Shuzhen1, ZHEN Junhui2, LI Qian1, XU Yihuai1   

  1. 1. Department of Pediatric Nephrology and Rheumatism and Immunology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-12-07 Online:2015-09-10 Published:2015-09-10
  • Contact: 孙书珍。E-mail:ssztml@163.com E-mail:ssztml@163.com

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Abstract: Objective To investigate the roles of chemokine ligand 16 (CXCL16) and oxidized low density lipoprotein (ox-LDL) in mice model with adriamycin induced nephropathy and their relationship with the renal tissue morphology, in order to explore the renal protective effects of simvastatin on adriamycin nephropathy. Methods We divided the mice into the following three groups: control group (n=5), adriamycin nephropathy group (n=5) and simvastatin treated adriamycin nephropathy group (n=5). Each group was treated with corresponding treatment respectively. 5 weeks after injection of adriamycin, the blood and 24 h urine were collected for the detection of general biochemical parameters and serum ox-LDL. After blood and 24 h urine collected, the mice were weighed and sacrificed with kidneys harvested. The renal morphology was observed by light microscopy and transmission electron microscopy, the expressions of CXCL16 and ox-LDL in podocytes were detected by double immunofluorescence techniques. Results Compared with the control group, the levels of TC and ox-LDL in the adriamycin nephropathy and simvastatin treated adriamycin nephropathy were significantly higher (P<0.05), the expressions of CXCL16 and ox-LDL in renal tissue were significantly increased (P<0.05). Compared with the adriamycin nephropathy group, the expressions of renal CXCL16 and ox-LDL in simvastatin treated adriamycin nephropathy group were markedly reduced (P<0.05), but there were no significant differences found in serum TC and ox-LDL between adriamycin nephropathy and simvastatin treated adriamycin nephropathy group. Conclusion Simvastatin has a protective effect on kidney in mice with adriamycin nephropathy. The mechanism might be related to the decreasing expression of CXCL16 in glomerular podocytes, followed by the decreasing endocytosis of ox-LDL in podocytes.

Key words: Nephropathy, Oxidized low density lipoprotein, Adriamycin, Simvastatin, Chemokine ligand 16

CLC Number: 

  • R726.9
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