JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (5): 36-42.doi: 10.6040/j.issn.1671-7554.0.2017.210

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TIPE3 decreases breast cancer cell sensitivity to adriamycin by upregulating P-gp protein expression

LIAN Kaili, LI Yan, ZHANG Na, JIN Guiyuan, LIU Suxia   

  1. Department of Immunology, School of Basic Medical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Received:2017-03-17 Online:2017-05-10 Published:2017-05-10

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Abstract: Objective To investigate the mechanisms of tumor necrosis factor-α induced protein 8 like 3(TIPE3), a recently investigated oncogenic transfer protein, on modulating breast cancer cell MCF-7 sensitivity to adriamycin. Methods TIPE3-overexpressed MCF-7 cells were treated with 0, 0.5, 1.0, 1.5 μg/mL adriamycin. After that, the relative viability and cell cycle of MCF-7 cells were detected with CCK-8 method and flow cytometry; the apoptosis of MCF-7 cells was assessed with Annexin V/PI; expression of MDR 1/P-gp and activation of NF-κB signal pathway were detected with qRT-PCR and Western blotting. Results Overexpressed TIPE3 promoted MCF-7 cell proliferation, increased the number of G2/M cells and inhibited apoptosis. Furthermore, overexpressed TIPE3 protein upregulated the activation of NF-κB signal pathway and the expression of MDR1/P-gp. Conclusion TIPE3 decreased MCF-7 cells sensitivity to adriamycin by upregulating the expression of P-gp via NF-κB pathway.

Key words: Nuclear factor-kappa B, Adriamycin, Tumor necrosis factor-α induced protein 8 like 3, Breast cancer, P-gp

CLC Number: 

  • R392.11
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