Abstract: Objective To observe the role of nuclear factor-kappa B(NF-κB)and NF-E2-related factor 2(Nrf2)in DHA antagonizing central nervous system(CNS)neurotoxicity of 1-bromopropane(1-BP). Methods A total of 48 Wistar rats were randomly divided into control group, 1-BP group, 250 mg/kg DHA+1-BP group(LDHA group)and 500 mg/kg DHA+1-BP group(HDHA group). 1-BP and DHA were orally administered to all animals for 12 consecutive days. The prefrontal cortex cytoplasmic protein activation of NF-κB, Nrf2, phase II enzyme protein, activation of astrocyte and 4-hydroxy-2-nonenal(4-HNE)modified protein were measured with Western blotting and immunofluorescence. Results The cortex cytoplasmic and nuclear protein expressions of NF-κB, Nrf2 and phase II enzyme protein were significantly higher in the control group than in 1-BP group(P<0.05). Compared with the 1-BP group, the expression of NF-κB and II enzyme protein were significantly lower (P<0.05)but the level of Nrf2 was significantly higher in LDHA group and HDHA group(P<0.05). Compared with the control group, activated astrocytes in 1-BP group were significantly increased, while those in LDHA group and HDHA group were significantly decreased. The content of 4-HNE modified protein in 1-BP group was significantly higher than in the control group(P<0.05). Compared with the 1-BP group, the content of 4-HNE modified protein was significantly lower in LDHA group and HDHA group(P<0.05). Conclusion DHA may show antagonism against CNS toxicity of 1-BP by inhibiting NF-κB and activating Nrf2. 山 东 大 学 学 报 (医 学 版)54卷4期 -王增金,等.NF-κB和Nrf2在DHA拮抗1-溴丙烷中枢神经毒性中的作用 \=-

Key words: 1-bromopropane, Nuclear factor-kappa B, NF-E2-related factor 2, Docosahexaenoic acid, Central nervous system toxicity