Abstract: Objective To study the effect of ubiquitin-proteasome system(UPS)in 1-bromopropane(1-BP)induced peripheral neuropathy in rats. Methods A total of 30 male Wistar rats were randomly divided into 3 groups: control group, 400 mg/kg·bw group(low dose group), and 800. All rats received an equal volume of corn oil and 1-BP(dissolved in corn oil)once per day, 5 days per week, for 16 consecutive weeks. Trypsin-like, chymotrypsin-like and peptide-glutamyl peptide hydrolase(PGPH)-like proteinase activities were measured with fluorescence labeling method. The 4-HNE, MDA protein adducts level and 4-HNE protein adducts in vitro in different nerve tissues were detected with Western blotting. Results Compared with control group, the trypsin-like proteinase activity in sciatic nerve was significantly elevated in the low and high dose groups(P<0.01); chymotrypsin-like proteinase activity in cerebrum in the high dose group was markedly improved(P<0.01); PGPH-like proteinase activity in the cerebrum and sciatic nerve in the high dose group was remarkably increased(P<0.05); 4-HNE and MDA protein adducts levels in sciatic nerve in the low dose group were increased(P<0.01); 4-HNE and MDA protein adducts levels in cerebrum and sciatic nerve in the high dose group were increased(P<0.01); 1-BP promoted the 4-HNE induced oxidative modification in cerebrum, spinal cord and sciatic nerve tissue in a dose-dependent manner(P<0.05). Conclusion 1-BP exposure resulted in the oxidative damaged proteins in the nervous system, and further abnormal activation of UPS, which might be involved in 1-BP induced peripheral neuropathy. 山 东 大 学 学 报 (医 学 版)54卷4期 -杨俊林,等.蛋白酶体活性升高在1-BP致大鼠周围神经损伤中的作用 \=-

Key words: 1-Bromopropane, Peripheral neuropathy, 4-Hydoxy-2-Nonenal, Malondialdehyde, Ubiquitin-proteasome system