JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 6-11.doi: 10.6040/j.issn.1671-7554.0.2014.934

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Effect of bufalin on chemosensitivity to cisplatin in non-small cell lung cancer and the underlying mechanisms

YANG Lu, LIU Yanguo, LI Jisheng, WANG Xiuwen   

  1. Department of Medical Oncology, Cancer Center, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-12-10 Revised:2015-01-23 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To investigate the effect of bufalin on chemosensitivity to cisplatin in non-small cell lung cancer (NSCLC) A549 and H1975 cells and explore the underlying molecular mechanisms. Methods A549 and H1975 cells were treated with various concentrations of genistein, cisplatin and their combination respectively. CCK-8 assay and flow cytometric analysis were used to investigate their effect on cell proliferation and apoptosis; Western blotting analysis was used to investigate the expressions of nuclear factor-kappa B(NF-κB) and its downstream molecule c-myc. Results Bufalin significantly inhibited the proliferation and induced apoptosis of A549 and H1975 cells in a time-and dose-dependent manner. Low concentration of bufalin significantly increased chemosensitivity to cisplatin in A549 and H1975 cells, accompanied by down-regulation of NF-κB and its downstream molecule c-myc. Conclusion Bufalin significantly increased chemosensitivity to cisplatin in A549 and H1975 cells, which is partly through inhibiting the activation of NF-κB and down-regulating its downstream molecular c-myc.

Key words: Chemosensitivity, Non-small cell lung cancer, Nuclear factor-kappa B/c-myc, Bufalin, Cell apoptosis, Cisplatin

CLC Number: 

  • R734.2
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