JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 12-16.doi: 10.6040/j.issn.1671-7554.0.2014.766

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Label-free quantification of proteomic changes in ethanol-induced hepatocyte

WANG Xiaoyue, WANG Daoguang, GUO Jisheng, LI Cuiling, JING Ruirui, LÜ Xin, CHEN Xinjun, YANG Jinghua   

  1. Cancer Research Center, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-10-30 Revised:2014-12-25 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To explore the proteomic changes in ethanol-induced hepatocyte cell line by a label-free quantification method. Methods Hepatocellular carcinoma cell line HepG2 was treated with ethanol and underwent in-solution-digestion. The total peptides were detected by liquid chromatography-mass spectrometry (LC-MS) and analyzed by Progenesis LC-MS, a commercial label-free quantification software. The differentially expressed proteins were further counted by Panther 9.0 and String 9.1 databases. Results More than 309 proteins were defined with significant difference in amount by Progenesis LC-MS. They were belong to catalytic enzymes, cell-signaling-related proteins, structural proteins, and soon which were involved in a variety of intracellular physiological responses, such as apoptosis and immune response. String showed there was a close relationship among these proteins. Conclusion Label-free quantification is an effective way of researching the mechanism of ethanol-induced hepatocyte damage.

Key words: Mass spectrometry, Label-free quantification, Proteomics, Hepatocyte damage, Ethanol

CLC Number: 

  • R394.3
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