JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 22-26.doi: 10.6040/j.issn.1671-7554.0.2014.800

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Mass spectrometry analysis of differentially expressed proteins between HPV immortalized cell line and SiHa cell line

LIU Yafei, ZHANG Qishu, YUAN Shoudao, Jason J. Chen   

  1. Cancer Research Centre, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-08 Revised:2015-01-23 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To explore the mechanism by which human papilloma virus (HPV) induces cervical cancer and to identify proteins differentially expressed between HPV immortalized cells and SiHa cells. Methods Differentially expressed proteins between HPV immortalized cells and SiHa cells were identified by high performance liquid chromatography and mass spectrometry, and analyzed by label-free quantity software and bioinformatics approaches. Results A total of 10 691 proteins were identified and 6 342 succinylation sites were found; label-free quantification analyses indicated that 572 proteins were differentially expressed between the two types of cells; bioinfomatic analysis showed that 273 proteins in HPV immortalized cells were mostly associated with disease pathway, while 299 proteins in cervical cancer cells were mostly involved in metabolic and protein-produced pathway. Although succinylation modifications had no differences, a new succinylation site at SGK1 K41 was found. Conclusion Compared with HPV immortalized cells, SiHa cells demonstrate higher level of metabolism and stronger protein-synthesis, while most up-regulated proteins in HPV immortalized cell are associated with particular diseases.

Key words: Proteomics, Human papilloma virus, Mass spectrometry, Cervical neoplasm

CLC Number: 

  • R737.33
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