JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 14-21.doi: 10.6040/j.issn.1671-7554.0.2014.023

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Mass spectrometry analysis of proteins differentially expressed between adenocarcinoma and squamous carcinoma of the cervix

YUAN Shoudao, WANG Fengqin, ZHANG Jiao, ZHAO Weiming, Jason J. Chen   

  1. The Cancer Research Center of Shandong University, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-01-10 Revised:2014-07-01 Online:2014-08-10 Published:2014-08-10

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Abstract: Objective To explore the mechanism for poor prognosis and high invasion/metastasis of cervical adenocarcinoma, and to compare the protein expression profiles of cervical adenocarcinoma cell line and squamous carcinoma cell line. Methods Differentially expressed proteins between cervical adenocarcinoma HeLa cells and cervical squamous carcinoma SiHa cells were identified by high performance liquid chromatography-Orbitrap Elite/Velos Pro mass spectrometry and analyzed by bioinformatics approaches. Results A total of 2 373 high-credibility proteins were identified in the two cell lines, and 787 of them were expressed differentially. Notably, 10.8% of the up-regulated proteins in the adenocarcinoma HeLa cells were related to energy-producing metabolism. On the other hand, 14.3% of the down-regulated proteins in the adenocarcinoma HeLa cells were related to apoptosis. In addition, cytoskeletal proteins were also down-regulated in the adenocarcinoma cell line. Conclusion High energy-productivity and low apoptosis may be the important mechanisms underlining poor prognosis in cervical adenocarcinoma. Further more, reduced cell cytoskeletal structure stability may be responsible for the invasive nature of adenocarcinoma.

Key words: Proteomics, Cervical cancer, Mass spectrometry, Bioinformatics, Label-free quantification

CLC Number: 

  • R318.04
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