Table of Content

  

10 August 2014
Volume 52 Issue 8

Mechanism of human umbilical cord mesenchymal stem cells suppressing cholangiocarcinoma cell growth

LIU Juan, HAN Guoqing, LIU Hui, QIN Chengyong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  1-5.  doi:10.6040/j.issn.1671-7554.0.2013.684

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Objective To explore the effect of human umbilical cord mesenchymal stem cells on cholangiocarcinoma HCCC-9810 cells and its molecular mechanism. Methods HCCC-9810 cells were treated with conditioned media from umbilical cord mesenchymal stem cells. Cell proliferation and apoptosis were detected with co-culture systems, MTT assay and DNA fragmentation assay; the protein expressions were determined with Western blotting and immunoflorescence staining. Results Conditioned media from hUC-MSCs could inhibit proliferation and induce apoptosis of tumor cells in a dose- and time-dependent manner. The proliferation inhibition rate increased from 12.87% to 50.98%, whereas the apoptosis rate increased from 10.1% to 49.67%. Immunoblot analysis showed that the expressions of p-Akt and p-GSK-3β were down-regulated. Conclusion The hUC-MSCs conditioned media may suppress the growth of HCCC-9810 human cholangiocarcinoma cell line via PI3K/Akt pathway.

Evaluation of NSAIDs-induced gastropathy and therapeutic effect by confocal laser endomicroscopy

WANG Han, SHI Sha, CHEN Feixue, LI Yueyue, CAO Jing, ZUO Xiuli, LI Yanqing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  6-13.  doi:10.6040/j.issn.1671-7554.0.2013.764

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Objective To explore the morphological nature and exact mechanisms of gastric mucosal barrier dysfunction underlying NSAIDs-related gastric damage by using confocal laser endomicroscopy (CLE), and to investigate how acute mucosal injuries were influenced by geranylgeranylacetone and rabeprazole. Methods After a 4-grade score system of acute gastric mucosal injury by CLE was established, the severity of NSAIDs-induced gastropathy and effect of medication were evaluated. The expressions of occludin and ZO-1 were detected. Results The CLE score was significantly increased and expression of tight junctions was down-regulated after the administration of indomethacin. Geranylgeranylacetone and rabeprazole could intervene in the damage process and protect the integrity of the epithelial barrier. Conclusion CLE can be objective, real-time and accurate in assessing the severity of NSAIDs-induced gastropathy. Geranylgeranylacetone and rabeprazole can be used as efficient prophylactic and therapeutic medications in NSAIDs-related gastropathy for their protective effect in maintaining the integrity of gastric mucosal barrier.

Mass spectrometry analysis of proteins differentially expressed between adenocarcinoma and squamous carcinoma of the cervix

YUAN Shoudao, WANG Fengqin, ZHANG Jiao, ZHAO Weiming, Jason J. Chen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  14-21.  doi:10.6040/j.issn.1671-7554.0.2014.023

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Objective To explore the mechanism for poor prognosis and high invasion/metastasis of cervical adenocarcinoma, and to compare the protein expression profiles of cervical adenocarcinoma cell line and squamous carcinoma cell line. Methods Differentially expressed proteins between cervical adenocarcinoma HeLa cells and cervical squamous carcinoma SiHa cells were identified by high performance liquid chromatography-Orbitrap Elite/Velos Pro mass spectrometry and analyzed by bioinformatics approaches. Results A total of 2 373 high-credibility proteins were identified in the two cell lines, and 787 of them were expressed differentially. Notably, 10.8% of the up-regulated proteins in the adenocarcinoma HeLa cells were related to energy-producing metabolism. On the other hand, 14.3% of the down-regulated proteins in the adenocarcinoma HeLa cells were related to apoptosis. In addition, cytoskeletal proteins were also down-regulated in the adenocarcinoma cell line. Conclusion High energy-productivity and low apoptosis may be the important mechanisms underlining poor prognosis in cervical adenocarcinoma. Further more, reduced cell cytoskeletal structure stability may be responsible for the invasive nature of adenocarcinoma.

Effect of parathyroid hormone_1-34 on bone healing around dental implants in ostoeporotic rats

DI Jing, SHI Xiaoting, LI Xue, LIN Zhiyong, SUN Zhe, DENG Fengying

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  22-26.  doi:10.6040/j.issn.1671-7554.0.2014.041

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Objective To investigate the effect of parathyroid hormone_1-34 (PTH_1-34) on bone healing around dental implants in ostoeporotic rats. Methods A total of 36 female Wistar rats were randomly divided into the sham-operation group, ovariectomized group and ovariectomized with PTH_1-34 treatment group, with 12 in each group. Animals in the sham-operation group underwent sham-operation, while rats in ovariectomized group and ovariectomized with PTH_1-34 treatment group received bilateral ovariectomy. Eight weeks after surgery, tibia of rats was examined for bone mineral density to verify osteoporosis status. At the same time, Pure screw titanium implant was placed in the right tibia near metaphysis. Every other day for 8 weeks, normal saline 1 mL/kg was intraperitoneally injected in rats in sham-operation group and ovariectomized group, while PTH_1-34 20 μg/kg in rats in the ovariectomized with PTH_1-34 treatment group. In week 4 and 8 after implantation surgery, half of the rats in each group were killed. Undecalcified sections were preparedand examined histologically and histomorphometrically. Results In week 4, the trabercular width (TW), implant bone contact rate (IBCR), combinded bone lamella width (CBLW) of rats in ovariectomized with PTH_1-34 treatment group were much higher than those of rats in ovariectomized group (P<0.05). In terms of IBCR, there was no difference between ovariectomized with PTH_1-34 treatment group and sham-operation group (P>0.05). In week 8, trabercular area (TA), TW, IBCR and CBLW were significantly elevated in ovariectomized with PTH_1-34 treatment group when compared with ovariectomized group (P<0.05). There was no difference between ovariectomized with PTH_1-34 treatment group and sham-operation group in all parameters (P>0.05). Conclusion Osteoporosis impedes osseointegration in ovariectomized rats. Intermittent application of parathyroid hormone_1-34 with small doses can promote bone formation effectively to improve the osseointegration quality of rats.

Effect of silencing TGF-β1 expression by small interfering RNA on ossification of the ligamentum flavum in mice

ZHANG Yingzhe, WU Dongjin, PENG Changliang, LI Bohan, SONG Yang, ZHANG Cheng, ZHAO Jie, LI Dequan, YANG Zhongyan, LIU Peng, ZHAO Kun, MA Shengzhong, GAO Chunzheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  27-33.  doi:10.6040/j.issn.1671-7554.0.2014.060

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Objective Transforming growth factor-β1 (TGF-β1) expression in fibroblasts of mice ligamentum flavum was inhibited by RNA interference (RNAi) technique, to investigate the effect of TGF-β1 on the ossification of ligamentum flavum. Methods Fibroblasts of mice ligamentum flavum were cultivated and ossification was induced with rhBMP-2 (recombinant human bone morphogenetic protein-2). After that, the osteoblasts were identified with morphologicalobservation, alkaline phosphatase staining and alizarin red staining of calcified nodules. Eukaryotic expression vector (siRNA-pSilencer2.0U6-TGFβ1) was constructed to transfect the osteoblasts, which were then divided into three groups. The experiment group was transfected with eukaryotic expression vector, the negative control group was transfected with vacant plasmid and the blank group was not treated. After that, the expressions of TGF-β1 and BMP-2 were detected by immunofluorescence technique before and after transfection; the expression change of TGF-β1 mRNA in osteoblasts was determined by Rt-PCR; the expression change of protein of TGF-β1 and BMP-2 was measured with Western blotting; the expression change of ALP and OC (osteocalcin) was assessed with enzyme linked immunosorbent assay (ELISA). Results After ossification had been induced successfully, ligamentum flavum cell morphological observation showed that alkaline phosphatase staining and alizarin redstaining of calcified nodules were both positive, and those cells had typical biological features of osteoblasts. After the osteoblasts were transfected by siRNA-pSilencer2.0U6-TGFβ1, immunofluorescence detection displayed decline in the fluorescence intensity of TGF-β1 and BMP-2. Compared with the negative control and bland control, Rt-PCR showed that the expression of the TGF-β1 mRNA in experiment group decreased significantly by 41.94% and 47.82%, respectively (P<0.01). Western blotting showed that the ratio of TGF-β1/β-action in experiment group decreased remarkably by 35.88% and 44.75%, respectively (P<0.01), and BMP-2/β-action decreased significantly by 81.79% and 86.06%, respectively (P<0.01). ELISA displayed that ALP in experiment group decreased notably by 24.14% and 32.30%, respectively (P<0.01), and OC decreased remarkably by 17.01% and 21.63%, respectively (P<0.01). Conclusion Eukaryotic expression vector (siRNA-pSilencer2.0U6-TGFβ1) could effectively inhibit the expression of TGF-β1 in osteoblasts and endogenous BMP-2, thus suppressing spinal ossification of ligamentum flavum.

Morphological changes and expressions of cleaved Caspase-3, PARP-1 in cerebellar fastigial nucleus of rats with vascular dementia

XIA Haimiao, SUI Rubo, ZHANG Lei, LIU Dandan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  34-38.  doi:10.6040/j.issn.1671-7554.0.2014.104

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Objective To observe the morphological changes and expressions of cleaved Caspase-3 and PARP-1 in the cerebellar fastigial nucleus of rats with vascular dementia (VD). Methods Thirty-two healthy Sprague-Dawley (SD) rats were randomly divided into two groups, sham group(n=8) and VD group(n=24). Morris water maze test was performed to evaluate the behavior of rats in sham group at week 1 and VD group at three time points(week 1, 2 and 4) after injury. The morphological changes of cerebellar fastigial nucleus were examined by Nissl staining. The expressions of cleaved caspase-3 and PARP-1 in the cerebellar fastigial nucleus were detected by immunohistochemical method. Results Compared with sham group, water maze escape latencies of VD group at three time points increased significantly (all P<0.01). Compared with sham group, the number of neurons in the cerebellar fastigial nucleus significantly decreased in VD groups (P<0.01). Compared with sham group, the expressions of cleaved Caspase-3 and PARP-1 in VD group significantly increased in the cerebellar fastigial nucleus (P<0.01). Conclusion The cerebellar fastigial nucleus is damaged in VD rats, while the cleaved caspase-3 and PARP-1 play an important role in this process.

Relationship between the mRNA expression of adipose triglyceride lipase in peripheral blood mononuclear cells and metabolic syndrome

JIN Chengwei, LI Kui, ZHAO Jing, YUE Xin, SHANG Yuanyuan, HAN Lu, ZHANG Yun, ZHANG Wei, MA Xiao, ZHONG Ming

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  39-42.  doi:10.6040/j.issn.1671-7554.0.2014.135

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Objective To determine the mRNA expression of adipose triglyceride lipase (ATGL) in peripheral blood mononuclear cell (PBMC), and to explore its role in the vascular injury of metabolic syndrome (MS). Methods ATGL mRNA expression in PBMCs was determined by real time quantitative PCR in 55 controls and 56 MS patients. All subjects underwent carotid ultrasonography to measure the intima-media thickness, pressure-strain elastic modulus and stiffness. Pearson correlation was applied to assess correlation between the mRNA relative expression of ATGL and age; body mass index; blood pressure; levels of triglycerides; high-density lipoprotein cholesterol; HOMA index. Stepwise linear regression analysis was performed to examine the relationship of intima-media thickness to clinical variablesand the relative expression levels of ATGL mRNA. Results Compared with the controls, MS patients had significantly increased mRNA expression of ATGL[(7.04±3.66) vs (2.25±1.69), (P<0.001)]. Multivariate linear regression analysis showed that age, elevated triglycerides and the homeostasis model assessment index were independent risk factors for ATGL mRNA expression. Furthermore, markedly increased intima-media thickness was also found in MS patients[(0.89±0.19)mm vs (0.56±0.12)mm, (P<0.001)]. Multivariate linear regression analysis indicated the increased triglycerides and ATGL mRNA expression were independent risk factors for intima-media thickness. Conclusion The ATGL mRNA expression in peripheral blood mononuclear is associated with the progression of vascular atherosclerosis in metabolic syndrome.

Study of diameter of ascending aorta and prosthesis-patient mismatch after aortic valve replacement

CHEN Lei, ZOU Chengwei, ZHANG Haizhou, ZHU Xiaolong, HAN Bo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  43-46.  doi:10.6040/j.issn.1671-7554.0.2014.038

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Objective To investigate changes of diameter of ascending aorta and the prosthesis-patient mismatch (PPM) after aortic valve replacement. Methods A total of 42 patients with aortic valve disease and ascending aortic dilatation (ascending aorta diameter 3.5 cm~5.0 cm) treated in our hospital during Dec. 2006 to Dec. 2012 were selected (cases with Marfan syndrome and ascending aortic dissection were excluded). The subjects were divided into bicuspid aortic valve group (group Ⅰ, n=22) and tricuspid aortic valve group (group Ⅱ, n=20) according to the features of aortic valves. According to the different types of valves, the subjects were divided into the mechanical valve group (group Ⅲ, n=26), and bioprosthetic valve group (group Ⅳ, n=16). All cases underwent simple aortic valve replacement surgery under cardiopulmonary bypass, without dealing with the dilated ascending aorta. The diameter of ascending aorta was measured and the effective orifice area index (EOAI) was calculated. According to EOAI, PPM was divided into three categories: mild (EOAI>0.85 cm²/m²), moderate (0.652/m²), and severe (EOAI≤0.65 cm²/m²). Results The ascending aorta change rates in group Ⅰ were: patients with aortic stenosis (0.6±1.09) mm/year, patients with aortic stenosis and regurgitation (0.5±0.6) mm/year, patients with aortic regurgitation(0.3±0.8) mm/year; the ascending aorta change rates in group Ⅱ were: patients with aortic stenosis (-0.2±1.2) mm/year, patients with aortic stenosis and regurgitation (-0.2±0.9) mm/year, patients with aortic regurgitation (-0.1±0.6) mm/year. Moderate PPM accounted for 15.38% in group Ⅲ and 43.75% in group Ⅳ. Conclusion Aortic valve replacement surgery is not adequant for BAV patients with aortic dilatation of the ascending aorta (aortic diameter 3.5 cm~5.0 cm), because postoperatively the ascending aorta will dilate to a certain degree. PPM occurs more often after aortic valve replacement and is likely to affect patients with bioprosthetic valve.

Association between calcineurin-nuclear factor of activated T cell pathway and atrial fibrillation in patients with valvular heart disease

LI Cong, WANG Jianchun, ZHAO Yong, ZOU Chengwei, SUN Hui, ZHANG Qing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  47-51.  doi:10.6040/j.issn.1671-7554.0.2014.052

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Objective To explore the association between calcineurin-nuclear factor of activated T cell pathway (CaN-NFAT pathway) and atrial fibrillation (AF) in patients with valvular heart disease (VHD). Methods A total of 43 patients with VHD who underwent valve replacement surgery were enrolled, including 24 with chronic persistent AF and 19 with sinus rhythm (SR). Peripheral venous blood samples were taken before surgery and cardiac muscle tissues were taken from the right atrial appendages during surgery. Blood samples were also collected from 20 healthy controls. Transcription of α- and β- isoforms of CaN subunit A (CnA) and NFATc3, NFATc4 in peripheral lymphocytes and right atrial appendages were assessed by real-time reverse transcription polymerase chain reaction (RT-PCR), with β-actin as an internal reference. Results Compared with SR patients and healthy controls, the mRNA levels ofCnA α-isoform, CnA β-isoform and NFATc3 were elevated in peripheral lymphocytes from AF patients (P<0.05). Compared with SR patients, the mRNA levels of CnA α-isoform, CnA β-isoform, NFATc3 and NFATc4 were elevated in cardiac tissues from AF patients (P<0.05). Conclusion CaN-NFAT pathway is associated with AF in patients with VHD.

Clinical observation of triple therapy of moxifloxacin and esomeprazole magnesium for Helicobacter Pylori

WANG Hui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  52-56.  doi:10.6040/j.issn.1671-7554.0.2013.701

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Objective To evaluate the efficacy of triple therapy of moxifloxacin and esomeprazole magnesium to eradicate Helicobacter pylori (Hp). Methods A total of 126 patients were randomly divided into moxifloxacin group and control group. The moxifloxacin group was treated with moxifloxacin, esomeprazole and clarithromycin, while the control group was treated with omeprazole, amoxicillin and clarithromycin. Both treatments lasted for 2 weeks. One month after the treatment, the patients undertook endoscopic examination (pathological biopsy), rapid urease test and ¹³carbon/¹⁴ carbon urea breath test (¹³C/¹⁴C-UBT). The adverse drug reactions and compliance were observed. Results In intention to treat (ITT)analysis, the Hp-eradication rate was 75.0% in the moxifloxacin group, and 58.1% in the control group (P<0.05); in per protocol (PP) analysis, the Hp-eradication rate was 92.3% in the moxifloxacin group, and 69.2% in the control group (P<0.05). However, in the adverse drug reaction and compliance comparison analysis, there was no statistical difference between the two groups (P>0.05). Conclusion The triple therapy based on moxifloxacin and esomeprazole is highly effective to eradicate Hp infection, with no significant difference in the adverse drug reaction and compliance from the conventional triple therapy; therefore, it is worth of clinical spread.

Expression of circulating miR-128 in serum of colorectal cancer and its effect on migration and invasion of colorectal cancer cells

ZHANG Yanli, LIU Xinfeng, ZHANG Xin, WANG Haiyan, YANG Yongmei, DU Lutao, WANG Lili, LI Peilong, WANG Chuanxin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  57-62.  doi:10.6040/j.issn.1671-7554.0.2013.773

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Objective To detect the expression of miR-128 in the serum of patients with colorectal cancer and to observe its effect on the migration and invasion of colorectal cancer cells. Methods The serum miR-128 levels were detected by quantitative real-time PCR in 57 cases of colorectal cancer and 20 healthy controls. The correlation between miR-182 expression and colorectal cancer metastasis was analyzed. The migration and invasion ability were observed by vitro experiments in HT-29 cells transfected with miR-128 mimics. Results The miR-128 expression in colorectal cancer was significantly lower than that in healthy controls (P<0.001), and it markedly decreased in the colorectal cancer metastasis group compared with non-metastasis group (P=0.014). The diagnosis and metastasis differential diagnosis capability of serum miR-128 for colorectal cancer was 0.85 and 0.71 (both P<0.05). Compared with the negative control group and blank control group, the migration healing rates and number of migration and invasion cells in the transfection group were significantly decreased (all P<0.05). Conclusion Circulating miR-128 is significantly decreased in serum of colorectal cancer and closely associated with colorectal cancer metastasis, which may provide a new idea for the diagnosis and therapy of colorectal cancer.

Difference of daily blood glucose profiles in hepatogenous diabetes and type 2 diabetes mellitus

XIAN Yuxin, SUN Ruixia, LI Li, LYU Wenshan, CAO Caixia, ZHANG Jietao, WANG Jing, GAO Yanyan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  63-67.  doi:10.6040/j.issn.1671-7554.0.2013.730

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Objective To study the characteristics of daily blood glucose profiles in hepatogenous diabetes (HD). Methods 20 newly diagnosed hepatogenous diabetes (HD group) and 20 newly diagnosed type 2 diabetes mellitus (DM2 group) patients were measured by continuous glucose monitoring system for 72 hours. Results There was no significant difference in the mean blood glucose in the two groups(P>0.05). In HD group, the levels of fasting plasma glucose and 3:00 blood glucose were significantly lower than those of DM2 group, and the levels of 2 h post-breakfast blood glucose(2 hPG) and postprandial glucose spike of breakfast were significantly higher than those of DM2 group (P<0.05). The standard deviation of blood glucose, mean amplitude of glycemic excursions and absolute means of daily difference were significantly higher in HD group than those of DM2 group(P<0.05). There was no significant difference in percentage of time spent in hyperglycemia above 11.1 mmol/L in the two groups(P>0.05); otherwise the area under the curve of postprandial glucose above 10 mmol/L in HD group was significantly higher than that of DM2 group (P<0.05). The level of fasting insulin in HD group was significantly higher than that of DM2 group (P<0.05), while there was no significant difference in the level of fasting C-peptide in the two groups(P>0.05). Conclusions Compared with type 2 diabetes mellitus, the hepatogenous diabetes have lower fasting plasma glucose and higher postprandial blood glucose. The time of postprandial glucose reaching peak is earlier, and the postprandial glucose spike of breakfast is higher than those of lunch and dinner. The daily blood glucose excursion is more obvious in the patients with hepatogenous diabetes.

Preliminary study of magnetic resonance intravoxel incoherent motion in the diagnosis of patients with mild cognitive impairment

DONG Dong, WANG Xinyi

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  68-71.  doi:10.6040/j.issn.1671-7554.0.2014.067

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Objective To investigate the difference of rCBF_ivim between the aged people who suffered mild cognitive impairment (MCI) and who showed normal cognition, and to evaluate the value of the intravoxel incoherent motion(IVIM) in the diagnosis of MCI. Methods The patients who visited the Neurology Department of Qianfoshan Hospital Affiliated to Shandong University from July 2012 to May 2013 with complaints of memory loss were enrolled in this study. Twenty MCI patients(MCI group) and 20 individuals with normal cognition(NC group) were performed IVIM detection. The values of D^* and f were measured in the areas of both sides of frontal, parietal, occipital lobe, temporal lobe and hippocampus, followed by the calculation of rCBF_ivim. Results Compared with NC group, rCBF_ivim significantly decreased in the frontal, parietal, temporal lobe and the area of hippocampus in MCI group (all P<0.05). Compared with NC group, rCBF_ivim of MCI group also decreased in the cerebellum and occipital lobe, which showed no statistical difference(all P>0.05). Conclusion The intravoxel incoherent motion technique is helpful for the diagnosis of MCI, and it is an useful tool for the assessment of cerebral blood flow in patients with MCI.

Clinical effect of hyperbaric oxygen with the addition of carbon dioxide inhaled through modified breathing equipment on patients with cerebral infarction

CUI Yan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  72-74.  doi:10.6040/j.issn.1671-7554.0.2014.076

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Objective To evaluate the clinical effects of hyperbaric oxygen with the addition of carbon dioxide inhaled through modified breathing equipment on patients with cerebral infarction and to assess the application value of the equipment. Methods Eighty patients with cerebral infarction were divided into hyperbaric oxygen with the addition of carbon dioxide group (HBO+CO₂ group) and hyperbaric oxygen group (HBO group). 98%O₂+2%CO₂ was adiministered in the HBO+CO₂ group, hyperbaric oxygen was adiministered in the HBO group. The score of National Institutes of Health Stroke Scale (NIHSS) and activity of daily life (ADL) were examined respectively before and after the treatment. Results Prior to the treatment, NIHSS scores and ADL scores showed no statistically difference between the two groups(all P>0.05). After the treatment, the NIHSS score was significantly lower and ADL score was significantly higher than those before treatment in both groups(all P<0.05). After the treatment, The NIHSS score of HBO+CO₂ group decreased and ADL score increased more evidently than those of the HBO group(all P<0.05). Conclusion Hyperbaric oxygen with the addition of carbon dioxide can not only play the role of hyperbaric oxygen but also eliminate vasoconstriction simultaneously, and thus improve neurological functions and life quality of patients with cerebral infarction.

Meta-analysis of TNFAIP3 gene polymorphism with rheumatoid arthritis

WANG Weiyan, YANG Qingrui, YUAN Yuan, YANG Xinglin, ZHANG Yuanchao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  75-80.  doi:10.6040/j.issn.1671-7554.0.2014.163

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Objective To validate the association between rheumatoid arthritis (RA) and TNFAIP3 gene polymorphism. Methods A total of 6 SNPs in TNFAIP3 (rs6920220, rs2230926, rs5029937, rs10499194, rs2327832, rs13207033) which had been extensively examined were selected. Published studies on RA and TNFAIP3 were retrieved and a Meta-analysis was conducted. Results Altogether 18 studies with 27 554 RA patients and 39,664 controls were included. The comprehensive analysis indicated that the gene polymorphism of rs6920220, rs2230926 and rs5029937 was associated with RA (P<0.000 01, P=0.03, P=0.002). The gene polymorphism of rs2327832 and rs13207033 was not associated with RA (P=0.27, P=0.05). The subgroup analysis revealed that the gene polymorphism of rs10499194 was associated with RA in white people [OR= 0.87 (0.80-0.95), P=0.002]. The gene polymorphism of rs10499194 was associated with RA in yellow people[OR=1.28(1.13-1.46), P=0.000 1]. Conclusion The Meta-analysis reveals that the gene polymorphism of rs6920220, rs2230926 and rs5029937 is associated with RA. The association between the gene polymorphism of rs10499194 and RA has racial differences.

3.0 T MR diffusion weighted imaging in staging of endometrial carcinoma and the correlation with pathology

DENG Kai, ZHANG Chengqi, LI Wei, WANG Guangli, DONG Guiqing, SHI Hao, PANG Tao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  81-84.  doi:10.6040/j.issn.1671-7554.0.2014.012

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Objective To investigate the value of 3.0 T diffusion weighted imaging (DWI) in staging of endometrial carcinoma and the correlation with pathology. Methods The data of 30 patients with histologically proved endometrial carcinoma were analyzed retrospectively. The staging diagnosis of DWI was compared with pathologic results，and the ADC values in different pathologic categories and differential grades of endometrial carcinoma were also compared. Results The staging accuracy of DWI was 83.3%. The ADC value in the 30 patients with endometrial carcinoma was (0.856±0.080)×10^-3 mm²/s. There was no significant difference in different pathologic categories(P>0.05). In differential grades of endometrial carcinoma, there was significant difference(P<0.01). Conclusion DWI has considerable value in staging of endometrial carcinoma, and the ADC value can preliminarily demonstrate the grade malignancy of the tumors.

A randomized prospective study on the clinical effect of two treatments on keloids

SUN Yukai, LI Zhonghua, WANG Haiqin, LU Changhui, YANG Bo, WANG Yimin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  85-89.  doi:10.6040/j.issn.1671-7554.0.2014.153

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Objective To evaluate the effect of two treatment approaches on keloids: fractional CO₂ laser combined with ³²P applicator, and fractional CO₂ laser combined with intralesional injection of compound betamethasone. Methods A total of 70 patients were divided into two groups: group A (n=36) treated with fractional CO₂ laser combined with ³²P applicator, and group B (n=34) treated with fractional CO₂ laser combined with intralesional injection of compound betamethasone. All patients received fractional CO₂ laser therapy first and then recombinant human epidermal growth factor gel. After the wound healed, patients in group A received ³²P applicator therapy, 0.4~0.8 MBQ/cm² for 96 hours; patients in group B received compound betamethasone injection therapy, once every month for 3 times. The clinical effect, symptoms, satisfaction and ratio of side effects between the two groups were compared. Results The cure rate and satisfaction in group A were, which were significantly higher than those in group B(χ²=5.884, t=2.193, P<0.05). The Vancouver Scar Scale (VSS) scores, pain scores, and itch scores were significantly improved in both groups after treatment (t=30.070, 14.797, 11.889; t=18.049, 10.016, 8.290, P<0.01); the above items in group A were significantly lower than those in group B after comprehensive intervention (t=5.861, 5.480, 3.069, P<0.01). Conclusion Fractional CO₂ laser combined with ³²P applicator is highly effective on keloids with satisfactory clinical results. However, the technique still needs improvement.

Prevalence and characteristics of tooth agenesis among orthodontic patients in Qinghai Province

HE Wucheng, LIU Ying, LI Zhenya

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  90-93.  doi:10.6040/j.issn.1671-7554.0.2014.019

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Objective To investigate the prevalence and characteristics of tooth agenesis among orthodontic patients treated in Xining First People's Hospital of Qinghai Province. Methods A total of 2 911 patients treated during 2007 to 2012 were selected, and divided into 3 groups: 1 missing-tooth group, 2 missing-teeth group and 3 missing-teeth group. Relevant data were collected and analyzed. Results The prevalence of congenital tooth agenesis was 7.7%, and tooth agenesis was more prevalent in females than in males (P<0.05). Altogether 95.6% patients missed 1-2 teeth, and the number of missing mandibular lateral incisor was 150 (44.2%). The lower mandible had higher percentage of missing teeth, while the left and right had similar percentage. There were significant differences among the 3 groups in the sites of missing teeth (P<0.01). Conclusions The prevalence of congenital tooth agenesis was 7.7% among orthodontic patients in Qinghai Province. The most common missing tooth was the mandibular lateral incisor, and majority of patients missed one or two teeth. When one or two teeth were missing, the site of missing teeth was more often the incisor area; when more than two teeth were missing, the site was more often the posterior area.

Composition and distribution of clinical yeasts in Guizhou region, China

WANG Meizhu, CAO Yu, ZHAO Liang, XU Xiaoshan, ZHANG Peifang, LV Qian, BAI Fengyan, KANG Yingqian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  94-97.  doi:10.6040/j.issn.1671-7554.0.2014.141

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Objectives To survey the species composition and distribution of clinical yeasts in Guizhou region. Methods Morphological observation, ITS1-SSCP patterning and sequencing were performed to identify 248 yeast strains from different clinical origins in Guizhou region, China. Results The identification result suggested that the isolated strains came from 2 genus and 10 species, in which the dominant genus and species were Candida and C.albicans, respectively. The molecular identification and CHROMagar testing coincidence rate was 91.53%. The distribution of different sources of clinical yeasts was significantly different in Guizhou region. Conclusion The combination of ITS1-SSCP and sequencing analyses can be accuracy and reliable when identifying the clinical yeasts with similar phenotypes. Although the most popular clinical yeast species was still C.albicans in local area, the increasing trend of non-albicans Candida species was noticed in this study, especially C.glabrata strains isolated from vulvovaginal andidiasis (VVC) patients.

Relationships between obese population-susceptibility and gene polymorphisms of neuropeptide Y and its receptor in a region of Henan Province, China

LIU Weigang, LU Yun, LI Linlin, LI Yunqing, SHENG Xuewen, YIN Lei, ZHANG Gongyuan, ZHAO Jingzhi

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  98-101.  doi:10.6040/j.issn.1671-7554.0.2013.541

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Objective To investigate the relationships between obese population-susceptibility and gene polymorphisms of neuropeptide Y and its receptors in a region of Henan Province, China. Methods We selected 510 inhabitants aged over 18 in a region of Henan Province by the method of random cluster sampling, and analyzed the relationships of obesity degree with genetic susceptibilities. Results The genic distributions of rs2880412 and rs2880416 had statistical differences (P<0.05). The rates of genic mutation and the distributions of genotypes showed the overall increasing trend (P<0.05). Three sites of different genotypes had relevance to the occurrence of disease. The distributions in three kinds of haplotypes, including CA, GA and GC had statistical differences between obese and non-obese groups (P<0.05). When haplotype CA was as a control, haplotypes GA and GC were relevant to obese susceptibility (P<0.05). Conclusion Gene polymorphisms of rs2880412 and rs2880416 sites are statistically relevant to obese susceptibilities. The corresponding allele C and C of them are risk factors of obesity. Haplotype-analysis showed that there are interactions in the three sites.

Relationships of dietary patterns with overweight, hypertension, hyperglycemia and hyperlipidemia in career women in Jinan

WEI Ying, SUN Yawen, GAO Youmei, XUE Fuzhong, YU Hongxia, ZHANG Chengqi

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2014, 52(8):  102-107.  doi:10.6040/j.issn.1671-7554.0.2013.712

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Objective To investigate the correlations between dietary patterns and overweight, hypertension, hyperglycemia and hyperlipidemia. Methods Nine hundred and nine women were selected to accept dietary survey and anthropometric and biochemical measures. Factor analysis was used to identify food patterns based on the dietary survey. Chi-square test and logistic regression were used to analyze the relation between food patterns and diseases. Results Four dietary patterns were identified: fish and vegetable pattern, oils pattern, fine grain and meat pattern and fruits and eggs patten. After adjusted by confounding factors, the highest quartile (Q4) of the fruits and eggs pattern was associated with lower risks of overweight and hypertension compared with the lowest quartile (Q1) (OR=0.05, 0.43, P<0.01). Q4 of the oils pattern was associated with higher risks of hyperglycemia and hyperlipidemia compared with the Q1 (OR=2.84, 4.35, P<0.01). Q4 of the fine grain and meat pattern was associated with higher risks of overweight, hypertension, hyperglycemia and hyperlipidemia compared with Q1 (OR=5.93, 2.28, 2.41, 6.03, P<0.01). Conclusions Dietary patterns were associated with chronic non-communicable diseases. Limiting intake of meat, increasing intake of vegetables and fruit, and taking whole grains instead of refined grains may play important roles in the prevention of chronic non-communicable diseases.