JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 34-38.doi: 10.6040/j.issn.1671-7554.0.2014.104

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Morphological changes and expressions of cleaved Caspase-3, PARP-1 in cerebellar fastigial nucleus of rats with vascular dementia

XIA Haimiao1, SUI Rubo2, ZHANG Lei1, LIU Dandan1   

  1. 1. Liaoning Medical College, Jinzhou 121001, Liaoning, China;
    2. Department of Neurology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning, China
  • Received:2014-02-28 Revised:2014-06-20 Online:2014-08-10 Published:2014-08-10

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Abstract: Objective To observe the morphological changes and expressions of cleaved Caspase-3 and PARP-1 in the cerebellar fastigial nucleus of rats with vascular dementia (VD). Methods Thirty-two healthy Sprague-Dawley (SD) rats were randomly divided into two groups, sham group(n=8) and VD group(n=24). Morris water maze test was performed to evaluate the behavior of rats in sham group at week 1 and VD group at three time points(week 1, 2 and 4) after injury. The morphological changes of cerebellar fastigial nucleus were examined by Nissl staining. The expressions of cleaved caspase-3 and PARP-1 in the cerebellar fastigial nucleus were detected by immunohistochemical method. Results Compared with sham group, water maze escape latencies of VD group at three time points increased significantly (all P<0.01). Compared with sham group, the number of neurons in the cerebellar fastigial nucleus significantly decreased in VD groups (P<0.01). Compared with sham group, the expressions of cleaved Caspase-3 and PARP-1 in VD group significantly increased in the cerebellar fastigial nucleus (P<0.01). Conclusion The cerebellar fastigial nucleus is damaged in VD rats, while the cleaved caspase-3 and PARP-1 play an important role in this process.

Key words: Fastigial nucleus, Vascular dementia, Caspase-3, PARP-1

CLC Number: 

  • R741.02
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