JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 26-30.doi: 10.6040/j.issn.1671-7554.0.2014.773

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Effects of thrombolytic therapy with broadened therapeutic window on the free radical activity and the expression of Caspase-3 in a rat model of acute cerebral infarction

LIU Jie, LIU Jinzhi, LIN Yan, HUANG Zhan, WANG Aihua   

  1. Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2014-10-31 Revised:2015-01-28 Online:2015-04-10 Published:2015-04-10

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Abstract: Objective To explore the effects of thrombolytic therapy with broadened therapeutic window on nitric oxide (NO), nitric-oxide synthase (NOS), malondialdehude (MDA), superoxide dismutase (SOD) and Caspase-3 in rats with acute cerebral infarction. Methods Rat models of middle cerebral artery occlusion (MCAO) were established with autologous thrombus. A total of 144 SD rats were randomly divided into control group, stroke group, and 6 h thrombolytic group (n=48 in each group). The contents of NO, NOS, MDA and SOD of cerebral tissue were assayed by the colorimetric and hydroxylamine methods. Changes of neurons were observed by HE staining. Expressions of neuronal NOS (nNOS) and Caspase-3 were determined by immunohistochemical techniques. Results Compared with stroke group, the contents of NO, inducible NOS (iNOS), MDA of 6 h thrombolytic group obviously decreased at different time points (P <0.05), the activity of SOD obviously increased (P< 0.05), neurons in the cortical brain increased, the expression of nNOS and Caspase-3 obviously decreased (P <0.05). Conclusion Thrombolytic therapy with broadened therapeutic window can decrease the reaction of oxidative stress and the expression of the apoptosis.

Key words: Cerebral infarction, Nitric-oxide synthase, Caspase-3, Thrombolysis, Time window

CLC Number: 

  • R743.3
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