JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 46-51.doi: 10.6040/j.issn.1671-7554.0.2014.731

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Effect of turmeric volatile oil on the proliferation and apoptosis of human leukemia cell line THP-1

TU Yunhua1, KANG Yingqian2, ZHOU Ying3, YE Zhenyuan1, XUE Yuecui1, DENG Renyuan1, WANG Meizhu2, CHEN Lan1, CAO Yu1   

  1. 1. Department of Dermatology and Sexually Transmitted Diseases, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou, China;
    2. Department of Microbiology, Guiyang Medical College, Guiyang 550004, Guizhou, China;
    3. Guizhou Province Chinese Medicine and Ethnic Medicine Creation Engineering Center, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China
  • Received:2014-10-21 Revised:2014-12-16 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To investigate the effect of turmeric volatile oil (TVO) on the apoptosis and proliferation of human leukemia cell line THP-1. Methods THP-1 Cells were incubated with different concentrations of TVO in vitro. The inhibition rate on cell proliferation was determined with methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The change of morphology was observed with inverted microscope after Giemsa's staining. Apoptosis was evaluated with DNA fragmentation and flow cytometry (FCM); cell cycle was analyzed with FCM. Caspase-3 activity was measured with a colorimetric method. Results TVO exhibited obvious inhibitive effect on the growth of THP-1 cells in a time-dose dependent manner. Meanwhile, TVO induced apoptosis of THP-1 cells in a dose-dependent manner. Typical morphology changes of apoptosis were presented. Caspase-3 activity was enhanced with high concentration of TVO. After TVO treatment, the number of cells decreased in G1/M phase and S phase but increased in G1 phase. Conclusion Turmeric volatile oil exhibits marked proliferation-inhibitive and apoptosis-inducing effect on THP-1 cells. The possible mechanism is to arrest cell cycle in G1 phase, and activate the key enzyme of apoptosis pathway.

Key words: Proliferation, Turmeric volatile oil, Apoptosis, Caspase-3, THP-1 cells

CLC Number: 

  • R932
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