JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (5): 31-35.doi: 10.6040/j.issn.1671-7554.0.2016.1293

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Effects of salinomycin combined with cisplatin on the proliferation and apoptosis of human gastric cancer cell line MKN-45

ZHAO Ming1,2,3, NIU Jie1, LI Fangqin3, WANG Aihong1,2, PANG Qiuxia1,2, CHEN Meini1,2, ZHOU Lizhen1, ZHAO Jumei1,2   

  1. 1. Medical College of Yanan University, Yanan 716000, Shaanxi, China;
    2. Key Laboratory of the Prevention and Treatment of Tumor, Yanan 716000, Shaanxi, China;
    3. Clinical laboratory of Affiliated Hospital of Yanan University, Yanan 716000, Shaanxi, China
  • Received:2016-10-12 Online:2017-05-10 Published:2017-05-10

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Abstract: Objective To investigate the effects and mechanisms of salinomycin(Sal)combined with cisplatin on the proliferation and apoptosis of human gastric cancer cell line MKN-45. Methods MKN-45 cells were cultured in vitro to logarithmic growth phase, and the proliferation rate of cell line MKN-45 preprocessed separately and jointly by Sal and cisplatin was detected with methyl thiazolyltetrazolium(MTT)method. The apoptosis were examined by AO/EB apoptosis kit and the protein expression levels of NF-κB and caspase-3 were detected with Western blotting. Results MTT assay indicated that the apoptosis rate of MKN-45 cells treated by the combination by Sal and cisplatin was obviously more than that by Sal and cisplatin used separately(P<0.05). AO/EB showed that administration of Sal and cisplatin respectively the apoptotic morphology was obvious compared with that of the control group, and the combined effect of the two drugs was more significant. Western blotting result showed that, after the Sal and cisplatin used in combination, the protein of NF-κB expression level decreased(P<0.05)and the protein caspase-3 increased(P< 山 东 大 学 学 报 (医 学 版)55卷5期 -赵明,等.盐霉素联合顺铂对人胃癌细胞MKN-45增殖和凋亡的影响 \=-0.05). Conclusion Sal suppresses gastric cancer cell proliferation, and combination of Sal with cisplatin would enhance the effect cisplatin does. The mechanism may be inhibiting the activation of NF-κB and up-regulation of the caspase-3 apoptotic gene in the downstream.

Key words: Salinomycin, Cisplatin, MKN-45, NF-κB, Caspase-3

CLC Number: 

  • R735.2
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