JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (2): 48-51.doi: 10.6040/j.issn.1671-7554.0.2013.260

• Articles • Previous Articles     Next Articles

Expression of the apoptosis-related protein in prefrontal cortex in a rat model of autism

WEN Min1, ZHAO Jing2, BAO Xing-xing3, DENG Cheng-min4, TONG Xue-tao3   

  1. 1. Department of Anatomy, Guiyang Medical University, Guiyang 550004, China;
    2. Department of Neurology, the Second People′s Hospital, Guiyang 550004, China;
    3. Department of Pediatrics, Affiliated Hospital of Guiyang Medical University, Guiyang 550004, China;
    4. Department of Biological Technology, Guiyang Medical University, Guiyang 550004, China
  • Received:2013-04-15 Online:2014-02-10 Published:2014-02-10

PDF (PC)

259

Abstract:

Objective   To investigate the change of apoptosis-related protein Caspase-3 and Bcl-2 and effect of apoptosis in the rat model of autism. Methods  Ten female Wistar rats (250-260 g) out of 20 were selected randomly to receive a single intraperitoneal injection of sodium valproate (VPA) at the pregnancy day of 12.5, whose offspring were defined as the autism model group. The rest 10 female Wistar rats received the intraperitoneal injection of saline, whose offspring were defined as the control group. To confirm whether the animal model was successfully established, the negative geotaxis test and self-grooming test were compared between the autism model group and control group. Western blotting was used to detect the expressions of Caspase-3 and Bcl-2 in prefrontal cortex in the two groups. Results  The animal model of autism was successfully established. Compared with the control group, the negative geotaxis time and the cumulative self-grooming time in the autism model group significantly increased (P<0.05); the expression of Caspase-3 decreased while Bcl-2 in prefrontal cortex incresed(all P＜0.05) in the autism model group. Conclusion  The apoptosis is inhibited in prefrontal cortex of autism, which suggests that the modulation of apoptosis might be a potential therapeutic strategy for autism.

Key words: Caspase-3, Autism, Bcl-2, Apoptosis

CLC Number: 

  • R729
[1] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[2] ZHANG Liang, XU Min, ZHUANG Xianghua, LOU Fuchen, LOU Nengjun, LÜ Li, GUO Wenjuan, ZHENG Fengjie, CHEN Shihong. An investigation of endoplasmic reticulum stress and apoptosis in diabetic peripheral neuropathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 13-17.
[3] ZHAO Ming, NIU Jie, LI Fangqin, WANG Aihong, PANG Qiuxia, CHEN Meini, ZHOU Lizhen, ZHAO Jumei. Effects of salinomycin combined with cisplatin on the proliferation and apoptosis of human gastric cancer cell line MKN-45 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 31-35.
[4] LI Rui, MA Weihong, REN Manyi, ZHAO Mengmeng, JIANG Shan, JU Yuanyuan, GUO Ying, SUN Zhaohui, SUI Shujian. Correlation between TWEAK and cardiac remodeling in essential hypertensive patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 49-55.
[5] GUO Hehe, SUN Zhiqiang, LIU Yanjuan, LIU Yichen, LI Guang, ZHENG Fang. Norcantharidin influences the expression of Notch signal pathway in multiple myeloma U226 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 32-37.
[6] TU Yunhua, CHEN Liyuan, WANG Xi, ZHOU Ying, KANG Yingqian, XUE Yuecui, RONG Dongyun, YE Zhenyuan, CAO Yu. Effect of ar-turmerone derivatives on proliferation and apoptosis in human melanoma WM35 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(2): 68-73.
[7] GONG Luwei, ZHOU Lizhen, SU Guohai. Perindopril ameliorates cardiac dysfunction via Akt-FoxO1 pathway in diabetic cardiomyopathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 65-70.
[8] ZHAO Lu, JIAO Jun, ZHANG Teng, JIAO Xinlin, CUI Baoxia. Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 49-53.
[9] ZHANG Kun, ZHAGN Xiaoguo, CUI Xingxing, CHEN Shijun. Expression of apoptosis-related genes in peripheral blood mononuclear cells of patients with HBV infection and the relationship with chronicity of hepatitis B [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 38-42.
[10] JU Yuanyuan, REN Manyi, LI Rui, ZHAO Mengmeng, SUI Shujian. Tumor necrosis factor-like weak inducer of apoptosis promotes expression of matrix metalloproteinase 2 and collagen Ⅰ in rat cardiac fibroblasts via the ERK1/2 signaling pathway [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(5): 23-28.
[11] LI Hongzhi, LIU Jing, SONG Yan, CHI Lingyi, LIU Yuguang. Role of liraglutide in the repair of spinal cord injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 1-5.
[12] REN Baoxin, MA Yunfeng, LIU Dianwei, LI Zhuo, JIANG Yong. Effects of Wnt3a on neuronal autophagy and apoptosis in early brain injury after subarachnoid hemorrhage in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 11-15.
[13] HAO Fengqin, LI Na. Neuroprotective effect of flavanoids extracted from onion on retinal ganglion cells with diabetes mellitus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 7-10.
[14] CHENG Xiangyu, XING Rui, XING Zhaoquan, GUO Zhaoxin, GUO Xiaoyu, SU Jing, MENG Liwei, LIU Zhaoxu. Effects of nitidine chloride on the proliferation and apoptosis of prostate cancer PC-3 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 13-18.
[15] GUO Changyan, QIN Xuejiao, WU Xiaoli, GAO Xue, CAI Keli. Protective effect of mitochondria-targeted peptide SS-31 against H2O2-induced apoptosis in human lens epithelial cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 23-26.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd