JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (8): 23-26.doi: 10.6040/j.issn.1671-7554.0.2014.195

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Protective effect of mitochondria-targeted peptide SS-31 against H2O2-induced apoptosis in human lens epithelial cells

GUO Changyan1, QIN Xuejiao1, WU Xiaoli2, GAO Xue3, CAI Keli1   

  1. 1. Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Ophthalmology, Shandong Rongjun Hospital, Jinan 250013, Shandong, China;
    3. Department of Ophthalmology, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2014-04-04 Published:2015-08-10

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Abstract: Objective To study the protective effect of SS-31 against H2O2-induced apoptosis in human lens epithelial cells and the possible mechanism. Methods SRA01/04 cells, an immortal human lens epithelial cell line, were cultured in vitro. SRA01/04 cells at the same generation were chosen and 100 μmol/L H2O2 was used to build the model of apoptosis. The cells were randomly allocated into five groups: the control group, the group treated by H2O2, and three groups pretreated by different concentrations of SS-31. The cell viabilities were monitored by using 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide(MTT) assay. The cell apoptotic rates were detected with Annexin V-Propidium iodide double-staining flow cytometry. The expressions of Bcl-2, Bax and Cleaved-Caspase-3 were tested by using Western blotting. Results After exposure of 100 μmol/L H2O2 for 24 h, the cell viabilities decreased, cell apoptotic rates increased, expression of pro-apoptotic protein Bax increased, expression of anti-apoptotic protein Bcl-2 decreased, and expression of Cleaved-Caspase-3 increased. Pretreatment of SS-31 at different concentrations improved cell viabilities in a dose-dependent manner. Meanwhile, SS-31 plays an anti-apoptotic role by decreasing cell apoptotic rate, down-regulating the expression of Bax, up-regulating the expression of Bcl-2, and suppressing the activation of Cleaved-Caspase-3. Conclusion SS-31 efficiently protects human lens epithelial cells against H2O2-induced apoptosis. Further studies are needed on whether SS-31 can be used in medicine treatment for cataract.

Key words: SS-31, Human lens epithelial cells, Apoptosis, Mitochondria-targeted peptide, Oxidative stress

CLC Number: 

  • R776.1
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