JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (3): 32-37.doi: 10.6040/j.issn.1671-7554.0.2016.458

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Norcantharidin influences the expression of Notch signal pathway in multiple myeloma U226 cells

GUO Hehe1, SUN Zhiqiang2,3, LIU Yanjuan1, LIU Yichen1, LI Guang1, ZHENG Fang2,3   

  1. 1. Graduate School, Guizhou Medical University, Guiyang 550004, Guizhou, China;
    2. Department of Hematology, Baiyun Affiliated Hospital of Guizhou Medical University, Guiyang 550014, Guizhou, China;
    3. Department of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China
  • Received:2016-04-25 Online:2017-03-10 Published:2017-03-10

Abstract: Objective To investigate the effects of norcantharidin(NCTD)on the expression of Notch signal pathway in multiple myeloma U226 cells. Methods U226 cells were co-cultured with NCTD at various concentrations in vitro. The proliferation inhibition was measured with CCK-8 test. The cell apoptosis rate was analyzed with flow cytometry. The mRNA and protein expressions of Notch2, Hes1, CyclinD1 and P21 in U266 cells were detected with qRT-PCR and Western blotting. Results NCTD could inhibit the proliferation of U266 cells and induce the apoptosis of cells in a time- and concentration-dependent manner. The mRNA and protein expressions(Notch2, P21)elevated with increased concentration of NCTD, while the expression of Hes1 and CyclinD1 decreased. Conclusion NCTD can inhibit the proliferation and induce the apoptosis of U266 cells, which may be related to the up-regulation of Notch2 and P21, and the down-regulation of CyclinD1 and Hes1.

Key words: Multiple myeloma, Notch signal pathway, Norcantharidin, U266 cells, Apoptosis

CLC Number: 

  • R932
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