JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 31-36.doi: 10.6040/j.issn.1671-7554.0.2014.775

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Involvement of canonical Wnt pathway in postischemic angiogenesis in a rat model of ischemic stroke

CHEN Haili, GU Jiaoyang, ZHANG Wenjing, YUAN Linran, ZHENG Juan, YUAN Zhongrui   

  1. Department of Pathophysiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-03 Revised:2015-01-05 Online:2015-04-10 Published:2015-04-10

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Abstract: Objective To investigate the involvement of canonical Wnt pathway in postischemic angiogenesis by using a rat model of ischemic stroke. Methods A total of 50 normal male Wistar rats were randomly divided into 4 groups: Sham group (n=10), cerebral ischemia group (MCAO group, n=20), LiCl treated group (LiCl group, n=10) and DKK1 treated group (DKK1 group, n=10). Western blotting was used to detect the expression of the elements of canonical Wnt pathway. FITC-dextran was used to detect the vessel volume, and Laser Doppler was used to measure the local cerebral blood flow in ischemic penumbral. Results The expressions of β-catenin in both the nuclear fraction and the cytoplasmic fraction in MCAO group decreased at 1 day post-cerebral ischemia, compared with Sham group(P< 0.05). Nevertheless, the expressions of β-catenin in both the nuclear fraction and the cytoplasmic fraction at 7 day post-cerebral ischemia significantly increased (P< 0.05), and reached normal level at 14 day post-cerebral ischemia. Compared with MCAO group, the expressions of β-catenin in both the nuclear fraction and the cytoplasmic fraction LiCl group in were upregulated, accompanied with the elevated ratio of p-GSK-3β/GSK-3β, enhanced vessel volume and local cerebral blood flow in ischemic penumbral(P< 0.05). Compared with MCAO group, the expressions of β-catenin in DKK1 group in both the nuclear fraction and the cytoplasmic fraction were downregulated, accompanied with the decreased ratio of p-GSK-3β/GSK-3β, weakened vessel volume and local cerebral blood flow in ischemic penumbral(P< 0.05). Conclusion The canonical Wnt pathway might be involved in postischemic angiogenesis.

Key words: Canonical Wnt pathway, Rats, β-catenin, Angiogenesis, Ischemic stroke

CLC Number: 

  • R614.2
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