JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 57-62.doi: 10.6040/j.issn.1671-7554.0.2013.773

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Expression of circulating miR-128 in serum of colorectal cancer and its effect on migration and invasion of colorectal cancer cells

ZHANG Yanli1, LIU Xinfeng1, ZHANG Xin2, WANG Haiyan2, YANG Yongmei2, DU Lutao2, WANG Lili2, LI Peilong2, WANG Chuanxin2   

  1. 1. Department of Clinical Laboratory, Traffic Hospital of Shandong Province, Jinan 250031, Shandong, China;
    2. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2013-12-27 Revised:2014-07-02 Online:2014-08-10 Published:2014-08-10

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Abstract: Objective To detect the expression of miR-128 in the serum of patients with colorectal cancer and to observe its effect on the migration and invasion of colorectal cancer cells. Methods The serum miR-128 levels were detected by quantitative real-time PCR in 57 cases of colorectal cancer and 20 healthy controls. The correlation between miR-182 expression and colorectal cancer metastasis was analyzed. The migration and invasion ability were observed by vitro experiments in HT-29 cells transfected with miR-128 mimics. Results The miR-128 expression in colorectal cancer was significantly lower than that in healthy controls (P<0.001), and it markedly decreased in the colorectal cancer metastasis group compared with non-metastasis group (P=0.014). The diagnosis and metastasis differential diagnosis capability of serum miR-128 for colorectal cancer was 0.85 and 0.71 (both P<0.05). Compared with the negative control group and blank control group, the migration healing rates and number of migration and invasion cells in the transfection group were significantly decreased (all P<0.05). Conclusion Circulating miR-128 is significantly decreased in serum of colorectal cancer and closely associated with colorectal cancer metastasis, which may provide a new idea for the diagnosis and therapy of colorectal cancer.

Key words: Metastasis, Invasion, Migration, Colorectal cancer, MiR-128

CLC Number: 

  • R735.3+4
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