JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (10): 41-45.doi: 10.6040/j.issn.1671-7554.0.2016.1522

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Effort of CD11b+ / CD66b- phenotype myeloid cells on the progression of colon cancer and liver metastasis

WANG Bishi1, SHAN Junqi1,2, HOU Qingsheng1, GONG Weipeng1, ZHU Zhenyu1, GUO Hongliang1   

  1. 1. The fourth department of surgical ward, Shandong Cancer Hospital, Jinan 250117, Shandong, China;
    2.School of Medicine and Life Science, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250022, Shandong, China
  • Received:2016-11-17 Online:2017-10-10 Published:2017-10-10

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Abstract: Objective To study the relationship between CD11b+/ CD66b- phenotype myeloid cells and colon cancer progression and liver metastasis. Methods The transcription level, protein translation levels of CD11b, chemokine C-C motif ligand 2(CCL2)and chemokine C-C motif receptor type 2(CCR2)in colonic carcinoma tissues, colorectal cancer tissues and liver metastasis were detected by real-time PCR, Western blotting and immunohistochemistry. The co-immunoprecipitation method was used to detect the relationship between CCL2 and CCR2. Results The expression of CD11b mRNA in colorectal carcinoma and liver metastasis was higher than that in normal tissues(P<0.05). The level of CCL2 mRNA transcription and protein expression in liver metastasis was significantly higher than that in normal colonic tissues and colon cancer tissues(P<0.05). The level of CCR2 mRNA transcription and protein expression in the three were no significant difference(P>0.05). Chemokine ligand CCL2 and chemokine receptor CCR2 can be combined with each other. Conclusion CD11b phenotype myeloid cell is extensive infiltration in colon cancer and liver metastasis of colon cancer tissue, and possibly can promote the progression of colon cancer and liver metastasis of colonic carcinoma by mediating with CCL2-CCR2 axis.

Key words: Liver metastasis, Chemokine C-C motif receptor type 2, Colorectal cancer, CD11b+/CD66b-Myeloid cells, Chemokine C-C motif ligand 2

CLC Number: 

  • R735.3+5
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