JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 47-51.doi: 10.6040/j.issn.1671-7554.0.2014.052

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Association between calcineurin-nuclear factor of activated T cell pathway and atrial fibrillation in patients with valvular heart disease

LI Cong1, WANG Jianchun1, ZHAO Yong1, ZOU Chengwei2, SUN Hui3, ZHANG Qing1   

  1. 1. Department of Geriatric Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    3. Department of Cardiology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2014-01-23 Revised:2014-04-11 Online:2014-08-10 Published:2014-08-10

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Abstract: Objective To explore the association between calcineurin-nuclear factor of activated T cell pathway (CaN-NFAT pathway) and atrial fibrillation (AF) in patients with valvular heart disease (VHD). Methods A total of 43 patients with VHD who underwent valve replacement surgery were enrolled, including 24 with chronic persistent AF and 19 with sinus rhythm (SR). Peripheral venous blood samples were taken before surgery and cardiac muscle tissues were taken from the right atrial appendages during surgery. Blood samples were also collected from 20 healthy controls. Transcription of α- and β- isoforms of CaN subunit A (CnA) and NFATc3, NFATc4 in peripheral lymphocytes and right atrial appendages were assessed by real-time reverse transcription polymerase chain reaction (RT-PCR), with β-actin as an internal reference. Results Compared with SR patients and healthy controls, the mRNA levels ofCnA α-isoform, CnA β-isoform and NFATc3 were elevated in peripheral lymphocytes from AF patients (P<0.05). Compared with SR patients, the mRNA levels of CnA α-isoform, CnA β-isoform, NFATc3 and NFATc4 were elevated in cardiac tissues from AF patients (P<0.05). Conclusion CaN-NFAT pathway is associated with AF in patients with VHD.

Key words: Atrial fibrillation, Valvular heart disease, Calcineurin, Nuclear factor of activated T cell, mRNA

CLC Number: 

  • R541.7
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