JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 69-74.doi: 10.6040/j.issn.1671-7554.0.2015.1146

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Significance of IRX5 mRNA in the diagnosis and prognosis of colorectal cancer

LIU Tong, ZHANG Xin, WANG Chuanxin   

  1. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-23 Online:2016-07-10 Published:2016-07-10

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Abstract: Objective To investigate the expression and clinical significance of iroquois homeobox(IRX5)mRNA in colorectal cancer(CRC). Methods Expressions of IRX5 mRNA were analyzed in 17 cases of inflammatory bowel diseases, 48 cases of hyperplastic polyp, 54 cases of colorectal adenoma, 142 CRC cases and 142 paired adjacent non-tumorous tissues by quantitative real-time polymerase chain reaction(RT-qPCR). The correlation between IRX5 mRNA expression and prognosis was analyzed. Results IRX5 mRNA expression was significantly elevated in the CRC group when compared with other groups(all P<0.001). In CRC, up-regulation of IRX5 mRNA expression was significantly correlated with tumor size, lymph node metastasis, and distant metastasis(all P<0.05). CRC patients with high IRX5 mRNA expression had poorer disease-free survival(DFS)and overall survival(OS)than those with low IRX5 mRNA expression(log-rank test, P<0.001). Further multivariable Cox regression analysis suggested that increased expression of IRX5 mRNA was an independent prognostic indicator for CRC(P=0.020, HR=1.863, 95%CI=1.101-3.152). Conclusion IRX5 mRNA expression is elevated in CRC, and associated with malignancy and prognosis of CRC, indicating that it may serve as a useful diagnostic and prognostic predictor.

Key words: Iroquois homeobox 5, Colorectal neoplasms, mRNA, RT-qPCR, Prognosis

CLC Number: 

  • R574
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