JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 60-68.doi: 10.6040/j.issn.1671-7554.0.2016.013

Previous Articles     Next Articles

Metabolomics analysis of colorectal cancer based on GC-TOF-MS

YU Fei, LIU Shaozhuang, ZHONG Mingwei, HUANG Xin, JIAO Jie, HU Sanyuan, YU Wenbin   

  1. Department of Surgery, Qilu Hospital Affiliated to Shandong University, Jinan 250012, Shandong, China
  • Received:2016-01-06 Online:2016-07-10 Published:2016-07-10

PDF (PC)

436

Abstract: Objective To explore the metabolic differences of cancer tissues, adjacent mucosa and normal mucosa in patients with colorectal cancer(CRC). Methods A total of 25 CRC patients were selected and the 3 kinds of tissues were taken from each patient. Gas chromatography time-of-flight mass spectrometry(GC-TOF-MS)platform was used for metabolomics analysis. The tissues were compared among each other, and principal components analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to analyze the data. Differential metabolites were mapped in the KEGG database to search for the related metabolic pathways. Results Compared with the normal mucosa, 47 metabolites were found different(P<0.05, q<0.05)in cancer tissues, and galactose metabolism, pentose phosphate pathway, glutamate and glutamine metabolism, glutamate, alanine and aspartate metabolism changed significantly(P<0.05). In the adjacent mucosa, 3 metabolites were found different(P<0.05, q<0.05), and galactose metabolism changed significantly(P<0.05). A total of 34 metabolites were found different(P<0.05, q<0.05)between the cancer tissues and the adjacent mucosa, and pentose phosphate pathway, glutamate and glutamine metabolism, glutamate, alanine and aspartate metabolism changed significantly(P<0.05). Conclusion The glucose metabolism and amino acid metabolism are changed in CRC tissues.

Key words: Metabolomics, Glucose metabolism, Colorectal neoplasms, Amino acid metabolism

CLC Number: 

  • R605
[1] Jemal A, Bray F, Center MM, et al. Global cancer statistics[J]. CA Cancer J Clin, 2011, 61(2): 69-90.
[2] 陈万青, 张思维, 曾红梅, 等. 中国 2010 年恶性肿瘤发病与死亡[J]. 中国肿瘤, 2014, 23(1): 1-10. CHEN Wanqing, ZHANG Siwei, ZENG Hongmei, et al. Report of cancer incidence and mortality in china, 2010[J]. China Cancer, 2014, 23(1): 1-10.
[3] 王宁,孙婷婷,郑荣寿,等.中国 2009 年结直肠癌发病和死亡资料分析[J].中国肿瘤, 2013, 22(7): 515-520. WANG Ning, SUN Tingting, ZHENG Rongshou, et al. An analysis of incidence and mortality of colorectal cancer in china, 2009[J]. China Cancer, 2013, 22(7): 515-520.
[4] Ni Y, Xie G, Jia W, et al. Metabonomics of human colorectal cancer: new approaches for early diagnosis and biomarker discovery[J]. J Proteome Res, 2014, 13(9): 3857-3870.
[5] Zhu J, Djukovic D, Deng L, et al. Colorectal cancer detection using targeted serum metabolic profiling[J]. J Proteome Res, 2014, 13(9): 4120-4130.
[6] Ma Y, Zhang P, Wang F, et al. An integrated proteomics and metabolomics approach for defining oncofetal biomarkers in the colorectal cancer[J]. Ann Surg, 2012, 255(4): 720-730.
[7] 马延磊, 刘伟杰, 彭佳远, 等. 基于气相色谱-质谱联用的结直肠癌代谢组模式特征性研究[J]. 中华胃肠外科杂志, 2009, 12(4): 386-390. MA Yanlei, LIU Weijie, PENG Jiayuan, et al. Study on specific metabonomic profiling of serum from colorectal cancer patients by gas chromatography-mass spectrometry[J]. Chin J Gastrointest Surg, 2009, 12(4): 386-390.
[8] Nishiumi S, Kobayashi T, Ikeda A, et al. A novel serum metabolomics-based diagnostic approach for colorectal cancer[J]. PLoS One, 2012, 7(7): e40459. doi:10.1371/journal.pone.0040459.
[9] Hung JS, Huang J, Lin YC, et al. C1GALT1 overexpression promotes the invasive behavior of colon cancer cells through modifying O-glycosylation of FGFR2[J]. Oncotarget, 2014, 5(8): 2096-2106.
[10] Williams MD, Zhang X, Belton AS, et al. HMGA1 drives metabolic reprogramming of intestinal epithelium during hyperproliferation, polyposis, and colorectal carcinogenesis[J]. J Proteome Res, 2015, 14(3): 1420-1431.
[11] Chan EC, Koh PK, Mal M, et al. Metabolic profiling of human colorectal cancer using high-resolution magic angle spinning nuclear magnetic resonance(HR-MAS NMR)spectroscopy and gas chromatography mass spectrometry(GC/MS)[J]. J Proteome Res, 2009, 8(1): 352-361.
[12] Mal M, Koh PK, Cheah PY, et al. Metabotyping of human colorectal cancer using two-dimensional gas chromatography mass spectrometry[J]. Anal Bioanal Chem, 2012, 403(2): 483-493.
[13] Qiu Y, Cai G, Zhou B, et al. A distinct metabolic signature of human colorectal cancer with prognostic potential[J]. Clin Cancer Res, 2014, 20(8): 2136-2146.
[14] Vizán P, Alcarraz-Vizán G, Díaz-Moralli S, et al. Modulation of pentose phosphate pathway during cell cycle progression in human colon adenocarcinoma cell Line HT29[J]. Int J Cancer, 2009, 124(12): 2789-2796.
[15] Shibuya N, Inoue K, Tanaka G, et al. Augmented pentose phosphate pathway plays critical roles in colorectal carcinomas[J]. Oncology, 2015, 88(5): 309-319.
[16] Huang F, Zhang Q, Ma H, et al. Expression of glutaminase is unregulated in colorectal cancer and of clinical significance[J]. Int J Clin Exp Pathol, 2014, 7(3): 1093-1100.
[17] Liu G, Zhu J, Yu M, et al. Glutamate dehydrogenase is a novel prognostic marker and predicts metastases in colorectal cancer patients[J]. J Transl Med, 2015, 13: 144.
[18] 张文静, 卿国良. 谷氨酰胺代谢与肿瘤[J]. 生命科学, 2013, 25(11): 1109-1114. ZHANG Wenjing, QING Guoliang. Glutamine metabolism and cancer[J]. Life of Science, 2013, 25(11): 1109-1114.
[19] Yang L, Moss T, Mangala LS, et al. Metabolic shifts toward glutamine regulate tumor growth, invasion and bioenergetics in ovarian cancer[J]. Mol Syst Biol, 2014, 10: 728. doi: 10.1002/msb.20134892.
[20] Ikeda A, Nishiumi S, Shinohara M, et al. Serum metabolomics as a novel diagnostic approach for gastrointestinal cancer[J]. Biomed Chromatogr, 2012, 26(5): 548-558.
[1] HU Yanwen, WANG Zhiyuan, YU Wanjiang, ZHAO Huichen, HAN Heli, XU Zhipeng, MA Hong, ZHANG Yuchao, LIU Yuantao. Correlation of fat distribution with metabolic syndrome and glucose metabolism in 52 obese patients [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 101-106.
[2] WANG Meijian, HOU Xinguo, LIANG Kai, CHEN Li. Serum lipids and lipoproteins in middle-aged and elderly Chinese in Shandong Province [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 70-75.
[3] CHENG Xiangdeng, LIANG Shuang, HU Yanyan, QIAO Yu, WANG Fengxue, LI Guimei. Hypothalamic-pituitary-gland axis function changes and metabolic manifestations of children with Prader-Willi syndrome [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 107-111.
[4] LIU Tong, ZHANG Xin, WANG Chuanxin. Significance of IRX5 mRNA in the diagnosis and prognosis of colorectal cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 69-74.
[5] LIU Yingjun, ZHANG Tao, WANG Lu, LIU Jia, CHANG Xuerun, ZHANG Jingxuan, XUE Fuzhong. Serum metabolic profiling of schizophrenia based on random forest [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 92-96.
[6] LIANG Kai1, HOU Xin-guo1, WANG Xin-hua2, SONG Yun-sheng2, JING Qing-ping3, QU Yong4, SUN Yu1, SONG Jun1, DONG Xiao-lin1, XU Yu-xin1, LI Zhen. Predictive values of anthropometric parameters on
glucose metabolism disorders [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(9): 59-62.
[7] CHENG Zhao-ling, LI Xue-mei, YANG Ting-ting, ZHU Qiang. Expression of TLR 4 in colorectal cancer and its
relationships with FasL and EGFR [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 92-.
[8] SUN Yanlai1, LI Zengjun1, ZHENG Yan2, XU Zhongfa1. The relationship of CXCR4 and MMP-9 expressions and clinico-pathologicalfactors with early recurrence after radical resection of colorectalcancer and their clinico-pathological significance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 108-112.
[9] MENG Yan1, CHEN Liyong2, XU Guifa1. Effects of resistant starch on intestinal function and aberrant crypt foci during the development of rat colorectal cancer induced by  Azoxymethane [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(4): 23-26.
[10] . Expressions of DcR3 and caspase3 and their significance in colorectal adenocarcinoma and precancerous lesions [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(8): 79-84.
[11] . Expression and significance of stem cell marker LGR5 proteins in the  development  and progression of human colorectal carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(8): 85-88.
[12] ZHU Ming-chen,ZHANG Yi,ZHAO Sheng-mei,LU Nan,WAN Fu-rong,LI Xiao-li,WANG Sheng-hua,LI Ying-jie,LI Hao,LIU Yang,ZOU Xiong. Expressions of HLA-A and B mRNA on peripheral blood mononuclear cells in colorectal cancer patients in tumorigenesis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(5): 490-493.
[13] SUN Hui,GONG Lei,LIU Bin,YANG Ya-pei,XU Chun-xiao,LIU Xian-xi. Cloning, expression and purification of human spermidine/spermine N1acetyltransferase gene [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(2): 128-131.
[14] LI Zeng-jun,SUN Yan-lai,LI Jian-ning,HAN Jian-jun. Recurrence-related factors following curative surgery for colorectal cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(8): 849-851.
[15] SUN Yu-ping,LIU Jie,CUI Xia,ZHAO Dong,LIU Hui-ping,WANG Yun-shan. Clinical significance of expression of B7H4 in colon and rectal cancer tissues [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(6): 590-592.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd