JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 60-68.doi: 10.6040/j.issn.1671-7554.0.2016.013

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Metabolomics analysis of colorectal cancer based on GC-TOF-MS

YU Fei, LIU Shaozhuang, ZHONG Mingwei, HUANG Xin, JIAO Jie, HU Sanyuan, YU Wenbin   

  1. Department of Surgery, Qilu Hospital Affiliated to Shandong University, Jinan 250012, Shandong, China
  • Received:2016-01-06 Online:2016-07-10 Published:2016-07-10

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Abstract: Objective To explore the metabolic differences of cancer tissues, adjacent mucosa and normal mucosa in patients with colorectal cancer(CRC). Methods A total of 25 CRC patients were selected and the 3 kinds of tissues were taken from each patient. Gas chromatography time-of-flight mass spectrometry(GC-TOF-MS)platform was used for metabolomics analysis. The tissues were compared among each other, and principal components analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to analyze the data. Differential metabolites were mapped in the KEGG database to search for the related metabolic pathways. Results Compared with the normal mucosa, 47 metabolites were found different(P<0.05, q<0.05)in cancer tissues, and galactose metabolism, pentose phosphate pathway, glutamate and glutamine metabolism, glutamate, alanine and aspartate metabolism changed significantly(P<0.05). In the adjacent mucosa, 3 metabolites were found different(P<0.05, q<0.05), and galactose metabolism changed significantly(P<0.05). A total of 34 metabolites were found different(P<0.05, q<0.05)between the cancer tissues and the adjacent mucosa, and pentose phosphate pathway, glutamate and glutamine metabolism, glutamate, alanine and aspartate metabolism changed significantly(P<0.05). Conclusion The glucose metabolism and amino acid metabolism are changed in CRC tissues.

Key words: Metabolomics, Glucose metabolism, Colorectal neoplasms, Amino acid metabolism

CLC Number: 

  • R605
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