JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 22-26.doi: 10.6040/j.issn.1671-7554.0.2014.041

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Effect of parathyroid hormone1-34 on bone healing around dental implants in ostoeporotic rats

DI Jing1, SHI Xiaoting2, LI Xue3, LIN Zhiyong1, SUN Zhe1, DENG Fengying1   

  1. 1. Department of Oral Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Pediatric Dentistry, Tianjin Stomatological Hospital, Tianjin 300041, China;
    3. Department of Stomatology, Shandong Medical College, Jinan 250014, Shandong, China
  • Received:2014-01-15 Revised:2014-06-10 Online:2014-08-10 Published:2014-08-10

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Abstract: Objective To investigate the effect of parathyroid hormone1-34 (PTH1-34) on bone healing around dental implants in ostoeporotic rats. Methods A total of 36 female Wistar rats were randomly divided into the sham-operation group, ovariectomized group and ovariectomized with PTH1-34 treatment group, with 12 in each group. Animals in the sham-operation group underwent sham-operation, while rats in ovariectomized group and ovariectomized with PTH1-34 treatment group received bilateral ovariectomy. Eight weeks after surgery, tibia of rats was examined for bone mineral density to verify osteoporosis status. At the same time, Pure screw titanium implant was placed in the right tibia near metaphysis. Every other day for 8 weeks, normal saline 1 mL/kg was intraperitoneally injected in rats in sham-operation group and ovariectomized group, while PTH1-34 20 μg/kg in rats in the ovariectomized with PTH1-34 treatment group. In week 4 and 8 after implantation surgery, half of the rats in each group were killed. Undecalcified sections were preparedand examined histologically and histomorphometrically. Results In week 4, the trabercular width (TW), implant bone contact rate (IBCR), combinded bone lamella width (CBLW) of rats in ovariectomized with PTH1-34 treatment group were much higher than those of rats in ovariectomized group (P<0.05). In terms of IBCR, there was no difference between ovariectomized with PTH1-34 treatment group and sham-operation group (P>0.05). In week 8, trabercular area (TA), TW, IBCR and CBLW were significantly elevated in ovariectomized with PTH1-34 treatment group when compared with ovariectomized group (P<0.05). There was no difference between ovariectomized with PTH1-34 treatment group and sham-operation group in all parameters (P>0.05). Conclusion Osteoporosis impedes osseointegration in ovariectomized rats. Intermittent application of parathyroid hormone1-34 with small doses can promote bone formation effectively to improve the osseointegration quality of rats.

Key words: Osteoporosis, Parathyroid hormone, Dental implants, Osseointegration

CLC Number: 

  • R782.1
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