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    Determination of the Benzo(a)pyrene level in cooking oil by solid
    phase extractionhigh performance liquid chromatography
    YANG Yan, FU Ya-qun, WU Xiao-meng, JIANG Qing-qing, SHAO Li-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (8): 124-127.  
    Abstract998)      PDF(pc) (1287KB)(121500)       Save

    Objective   To establish a high performance liquid chromatography with fluorescence detector for the determination of the benzo(a)pyrene(B(a)P) level in the cooking oil. Methods   The difference of B(a)P content in edible oil in Jinan market was investigated by the SHIMADZU shim-pack VP-ODS (250mm×4.6mm, 5μm) column. The mobile phase was methanol-water(90∶10, V/V ), and the flow rate was 1. 0mL/min. The samples were treated by the non-bonded silica solid phase extraction(SPE) column, and then detected at the 285nm excitation wavelength and 406nm emission wavelength. Results   The lowest limit of determination was 0.039μg/kg (S/N=3). The recovery rate was 85.8%-90.7%,  and the intra-day and inter-day relative standard deviations were 2.9%-4.1% and 3.7%-5.3%. Conclusion   This method is high sensitive, good selective and quantitative accurate, which is suitable for the B(a)P determination in the cooking oil.

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    Rosuvastatin reverses the effect of heavy drinking on angiogenesis after infarction in rats
    QU Fuchao, WANG Aihong, SUN Yongle, WANG Yong, YUAN Haitao, ZHANG Yuying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    DOI: 10.6040/j.issn.1671-7554.0.2013.552
    Heterogeneity of human peripheral and decidual monocytes of women in early pregnancy
    ZHANG Yun 1,2, SUN Jin-tang 2, HUANG Xian-liang 3, YANG Mei-xiang 2,SHAO Qian-qian 2, DENG Bi-ping 2, XU Xiao-fei 3,MAO Hai-ting 2, DONG Ba
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (3): 98-101.  
    Abstract911)      PDF(pc) (444KB)(2860)       Save

    Objective    To analyze the distribution of human CD14+CD16- and CD14+CD16+ monocyte subsets in peripheral blood and decidua of women in early pregnancy. Methods    Peripheral blood samples were collected from early pregnant and nonpregnant females. Mononuclear cells were isolated from the fresh decidua of early pregnant females by mechanical trituration and Ficoll gradient centrifugation. The fluorescent intensity of the cells was detected by FCM. Results    The percentage of the peripheral CD14+CD16- monocyte subtype in early pregnant females was lower than that in  non-pregnant controls(P<0.01). CD14+CD16+ cells (69.13%±7.73%)  and were the major monocyte subset in the decidua of early pregnant females. Conclusion    CD14+CD16+ monocytes become the predominant cell subpopulation of women in early pregnancy. It has been demonstrated that CD14+CD16+ monocyte-derived dendritic cells are efficient producers of anti-inflammatory interleukin-10, so CD14+CD16+ monocytes  might play an important role in forming and keeping the dominance of TH2 cytokine during early pregnancy.

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    Mucus fills the intestinal epithelial gaps and maintains the barrier function
    ZHOU Xiaoyan, LI Ming, GUO Jing, ZUO Xiuli, LI Yanqing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2015, 53 (12): 62-66,80.   DOI: 10.6040/j.issn.1671-7554.0.2015.148
    Abstract239)      PDF(pc) (3773KB)(1851)       Save
    Objective To investigate whether mucus fills the intestinal epithelial gaps(IEG), and to explore the role of mucus in maintaining local barrier function. Methods The terminal ileum tissues of ulcerative colitis (UC) patients and controls were obtained. Mucins were stained with Alcian Blue-Periodic Acid Schiff (AB-PAS) and Muc2. In addition, we also analyzed the confocal laser endomicroscopy (CLE) imagines of the terminal ileum from 39 UC patients and 34 controls since January 2013 to December 2014. Revised Watson grading system was used to evaluate the local barrier function. Results In UC patients, the proportion[M(P25,P75),%] of IEG without mucus, intestinal epithelial gaps, goblet cells were 27.52(25.00,29.68), 5.33(3.48,6.62) and 17.38(16.00,19.87), respectively. In control group, these three parameters were 12.27(9.78,16.67), 0.27(0.00,0.69) and 23.44(20.24,26.18), respectively. The differences were statistically significant in the two groups (P<0.01). The proportion of intestinal epithelial gaps and goblet cells showed a significantly negative correlation(r=-0.74, P<0.01). Conclusion The local barrier function is lost in UC patients; mucus filled with IEG play a key role to maintain the local intestinal barrier function.
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    Effect of dexmedetomidine on general anesthesia in
    hypertensive patients undergoing craniectomy
    ZHOU Ting-fa1, TANG Zhen-shen2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (8): 81-.  
    Abstract562)      PDF(pc) (1001KB)(1152)       Save

    Objective   To detect the effect of dexmedetomidine(DEX) on general anesthesia of hypertensive patients undergoing retromastoid craniectomy for microvascular decompression(MVD) of facial nerve. Methods   48 (ASA Ⅰ-Ⅱ) hemifacial spasm patients with hypertension undergoing retromastoid craniectomy for MVD of facial nerve under general anesthesia were randomly divided into two groups: the study group and the control group. Dexmedetomidine was injected with the load dosage (1μg/kg) before anesthesia, followed by the maintaining dosage [0.20.7μg/(kg·h)] during anesthesia in the study group. Sodium chloride injection was pumped in the control group. Anesthesia was maintained with propofol and remifentanil. During the surgery, systolic blood pressure (SBP) was maintained 70%-80% of the baseline value by titration of anesthetic drugs and/or nitroglycerin in both groups. The mean infusion rates of propofol and remifentanil, anesthesia recovery and extubation times were observed. Results   Compared with the control group, patients in the study group required lower normalized infusion rates of propofol [(0.076±0.014)mg/(kg·min) vs (0.092 ± 0.018)mg/(kg·min), P<0.05] and remifentanil [(0.106±0.032) vs (0.131±0.030)μg/(kg·min), P<0.05]. Anesthesia recovery times [(5.2±2.1)min vs (9.3±2.4)min] and extubation times [(5.3±2.4)min vs (10.3±3.2)min] were also significantly shorter than those in the control group (P< 0.05). Conclusion   Dexmedetomidine can effectively reduce the consumption of anesthetic drugs, shorten the recovery  times and hasten the extubation in the hypertensive patients during retromastoid craniectomy undergeneral anesthesia.

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    Effect of polypeptide extract from scorption venom on tumor growth and cellular immunity in rats with W256 sarcocarcinoma
    ZHANG Wei-dong,ZHANG Yue-ying,WANG Zhao-xia,WANG Zhao-peng,JIA Qin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1234)      PDF(pc) (267KB)(983)       Save
    Objective: To study the inhibitory mechanism of polypeptide extract from scorpion venom (PESV) on W256 sarcocarcinoma and its influence on T subsets in rats. Methods: Tumor models were established by subcutaneously implanting W256 tumor cells into thirty Wistar rats. The tumor-bearing rats were randomly divided into three groups: the model group, the 5-fluorouracil(5-FU) group, and the PESV group. PESV was intragastrically subjected to the rats of the experimental group for 7 days. Then, the tumor-bearing rats were killed, and T subsets in the peripheral blood were determined by flow cytometry (FCM). The presence of interferon-gamma (INF-γ) and interleukin-4 (IL-4) were screened by an enzyme-linked immunosorbent assay (ELISA). Results: PESV had an inhibitory effect on tumor growth, and the growth inhibitory rate was 55.2%. The number of CD4+T and the CD4+T/CD8+T ratio of W256-bearing rats treated with PESV significantly increased compared with the model group (P<0.05). The expression level of interferon-gamma (INF-γ) was up-regulated and of interleukin-4 (IL-4) down-regulated. Compared with the control group, the difference was significant(P<0.05). Conclusion: PESV has an inhibitory effect on tumor growth by strengthening the immunocellular function. It could be regarded as a candidate of tumor inhibition.
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    Actovegin restores the digestive tract in rats with acute radiation enteritis
    BI Ying-hui,HAN Jun-qing,SHENG Wei,LI Zhao-pei,HU Ping-ping,SHEN Bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1258)      PDF(pc) (817KB)(955)       Save
    Objective: To evaluate the effect of Actovegin in restoring the digestive tract in rats with acute radiation enteritis. Methods: Whole abdominal radiation was performed on Wistar rats. The rats were randomly divided into three groups: the Actovegin group (Actovegin injection), the control group(irradiation only), and the normal group (without irradiation). Morphologic indices were measured by using light microscopy and the image analysis system. Intestinal NO concentration was detected by nitric acid reductase and bacterial translocation was also examined. Results: The Actovegin group had a significantly higher level of the height of the villus, the depth of crypt, the thickness of the mucosa and the entire wall, the number of the metaphase mitoses per crypt, and a significantly lower level of bacterial translocation and NO concentration compared with the control group. Conclusion: Actovegin accelerates recovery of injured digestive mucosal epithelium and decreases intestinal NO concentration and is good for regaining normal structure and function of digestive mucosa.
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    Effect of gypenosides on proliferation of neural precursor cells in vitro
    XIE Shanshan1, WANG Yuzhuo1, SUN Tao1, ZHANG Daolai2, FENG Yuxin1, XIN Hua1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (2): 85-90.  
    Abstract942)      PDF(pc) (973KB)(901)       Save

    Objective  To study the effect of gypenosides (GPs) on proliferation of neural precursor cells (NPCs) in vitro. Methods  NPCs were isolated from the brains of embryonic rats on day14 of pregnancy. After adherent culture for 7 days, the cells were passaged for the first time, and cultured for another 3 days. We identified the purity of the NPCs by immunofluorescence technique, then incubated the NPCs together with GPs in different concentrations(0, 25, 50, 100, 200 and 400μg/mL)for 48 hours. After that the purity of the NPCs was again identified,  activity was measured by MTT chromatometry, a cell growth curve was drawn by cell counting,  the proliferation of NPCs was measured by bromodeoxyuridine (BrdU) incorporation, and determined the expression level of proliferating cell nuclear antigen (PCNA) was determined by Western blot. Results  The purity of the NPCs cultured for 3 days after being passaged for the first time was 97 %. Without changing the purity of NPCs, GPs increased the activity of NPCs, accelerated the growth of NPCs, improved the positivity rate of BrdU, and up-regulated the expression level of PCNA. Conclusion  GPs promote the proliferation of NPCs in vitro through increasing the expression level of PCNA, and the optimal concentration of GPs is 100μg/mL.

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    Signal quality assessment of ECG in the intensive care unit
    LI Qiao,YU Meng-sun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract936)      PDF(pc) (250KB)(885)       Save
    Objective: To develop an algorithm of signal quality assessment of ECG in the intensive care unit. Methods: The signal quality index (SQI) was obtained by analyzing the morphological and statistical characteristics of each waveform and their relationships. The heart rate was robustly estimated based on SQI. The algorithm was evaluated using more than 6?000 hours of simultaneously acquired ECG from a 437patient subset of the MultiParameter Intelligent Monitoring for Intensive Care II database and adding real ECG noises. Results: The SQI was decreased along with a decrease of the signal noise ratio. The SQI had high-positive correlation with the QRS detective sensitivity and positive predictivity. This method provided an accurate HR estimation even in the presence of high levels of persistent noises and artifacts. Conclusion: The signal quality assessment algorithm could provide an objective estimation of ECG quality.
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    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract956)      PDF(pc) (221KB)(882)       Save
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    Role of iodine on wounds in the skin of rats
    CHEN Li-xin,WANG Yi-bing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1172)      PDF(pc) (749KB)(879)       Save
    Objective: To study the effect of iodine on wounds in the skin of rats. Methods: A bilateral round full-thickness wound was made on the back of Wistar rats. The benefit effect of iodine on the skin wound was evaluated by determining closure time and contracting ability of the skin wound. The expression of VEGF in the wound tissues was determined by immunohistochemical techniques and an image analysis system. Results: The closure time of skin wounds was significantly shorter in the 10HI-treated groups than that in the sodium chloride-treated groups. The percentage of the wound contraction was higher in the 10HI-treated groups than that in the sodium chloride-treated groups (P<0.01 and P<0.05) between the 1st and the 7th day after wounding. There was no difference between the 1000HI-treated groups and the sodium chloride-treated groups. Histopathological results showed that 10HI could promote the formation of new born capillaries and the proliferation of fibroblasts at an early period of wound healing and promote the expression of VEGF from fibroblasts and the vascular endothelial cells; whereas 1000HI reversed these. Conclusion: Iodine of benefit concentration has the ability to promote healing in the skin of rats, but iodine of high concentration can delay healing.
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    NIU Fu-wen,NIE Lin,HOU Yong,CHENG Lei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract986)      PDF(pc) (183KB)(871)       Save
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    Effects on endothelial cell proliferation induced by different concentration of iodine in vitro
    WANG Xiao-lei,XU Li-ya,ZHANG Hai-tao,LI Jia-mei,GUO Cheng-hao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1255)      PDF(pc) (300KB)(862)       Save
    Objective: To research the effects on proliferation of human umbilical vein endothelial cells (HUVECs) induced by different doses of iodine. Methods: HUVECs were subjected to iodine in various concentrations (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 8.0μmol/L)for 24 hours, and the morphology of the cultured endothelium was observed. MTT colorimetry was used to determine the proliferation activities of the cultured endothelium. Results: Iodine significantly increased the number and MTT activities of vascular endothelial cells from 0.5μmol/L to 4.0μmol/L compared with the normal group at 24 hours. Cell morphology was normal under a microscope. The number and MTT activities of vascular endothelial cells were significantly increased by iodine at over 5.0μmol/L at 8 hours, but were decreased at 12 hours and later. Poison granules in the endothelium were produced by iodine at over 5.0μmol/L at 4 hours and increased in a dose-and time-manner. Conclusion: Iodine effects on the proliferation activity of endothelial cells are dose-and time-dependent.
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    Expression of MMP-9 and PCNA in sinonasal inverted papilloma and the clinical significance
    LIU He,ZOU Xue,LI Min,WU Xiao-juan,ZHANG Li-qiang,YE Ping,LI Xue-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1083)      PDF(pc) (509KB)(842)       Save
    Objective: To explore the function of MMP-9 and PCNA in the occurrence, recurrence and malignancy of the sinonasal inverted papilloma (SNIP). Methods: Forty specimens with SNIP and ten specimens with squamous cell carcinoma (SCC) were selected for this study. All the specimens were divided into four groups: the SNIP group, the recurrent SNIP group, the SNIP with carcinoma group, and the invasive squamous cell carcinoma (SCC) group. The expression of MMP-9 and PCNA was determined by using an immunohistochemistry method. The results were analyzed by a quantitative immunohistochemical analysis. Results: Expression of increased in the SNIP group, the recurrent SNIP group, the SNIP with carcinoma group, and the SCC group. According to the rank test, the expression of MMP-9 was significantly different between the SNIP group and other groups. But between recurrent SNIP and SNIP with carcinoma, recurrent SNIP and SCC, SNIP with carcinoma and SCC, there were no prominent differences. The expression of PCNA was not significantly different between any two groups. Conclusion: The expression of MMP-9 and PCNA is involved in the mechanism of the occurrence of SNIP and plays an important role in the recurrence and malignancy of SNIP. The over expression of MMP-9 and PCNA may be associated with early events in the recurrence tendency and carcinogenesis/transformation to SNIP.
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    Expression and signficance of TNF-related apoptosis inducing ligand and death receptor-5 in atherosclerosis rabbits
    FEI Ling,SUI Shu-jian,REN Man-yi,XU Fu-yu,LIU Wei-hua,DU Yi-meng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract937)      PDF(pc) (422KB)(837)       Save
    Objective: To explore whether TNF-related apoptosis inducing ligand(TRAIL)and death receptor-5(DR5) take part in the occurrence and development of atherosclerosis(AS). Methods: Twenty rabbits were randomly distributed into two groups: the control group and the atherosclerotic group. The experimental atherosclerotic models were made by endothelial injury on the ventro-aorta with a balloon and hyperlipid feeding lasting 12 weeks. The levels of sTRAIL and sDR5 in the serum were determined by enzyme linked immunoabsorbent assay (ELISA). The proteinum expression of TRAIL and DR5 in the ventro-aortas was detected by immunohistochemical technique. Results: In the course of research, 2 rabbits died. The survival rate was 90%. Till the end of the research, the serum sDR5 in the AS group was more significantly elevated than that in control group (P<0.05), while the serum sTRAIL in two groups had no significant differences (P>0.05). The expressions of TRAIL and DR5 in the ventro-aortas in the AS group were significantly elevated than those in the control group (P<0.05). Conclusion: This study suggests that TRAIL and DR5 could promote the occurrence and development of AS. TRAIL and DR5 may be important factors for AS.
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    Transcranial ultrasound-enhanced thrombolysis by urokinase for acute cerebral infarction in rats
    XIE Zhao-hong,ZHU Zheng-yu,YANG Hong-xia,BI Jian-zhong,LAI Chao,CONG Yan-bin,WANG Xiao-yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1223)      PDF(pc) (660KB)(837)       Save
    Objective: To observe the effect of transcranial ultrasound-enhanced thrombolysis by urokinase on acute cerebral infarction in rats. Methods: The animal model of acute cerebral infarction was made by injecting auto-thrombsis into the left middle cerebral artery. Intravenous urokinase and transcranial ultrasound were given at the same time. The functioning of the nervous system was measured by NSS. The infarct volume was measured after TTC staining. Pathological changes were observed by a light microscope and transmission electron microscope. Results: The NSS score of the large dose urokinase group and the ultrasound plus small dose urokinase group at 24 hours after treatment was lower than that before treatment(P<0.01) and of that of the infarct group(P<0.01). The infarct volume of the two groups was lower than that of the infarct group, but there were no differences between the two groups(P>0.05). Remission of pathology was seen under both light microscope and transmission electron microscope. Conclusions: Transcranial ultrasound enhanced thrombolysis by urokinase can accelerate the recovery of the nervous system in acute cerebral infarction rats, diminish the infarct volume, decrease the ischemic changes of the neurons, and reduce the dosage of urokinase.
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    Open MR-guided celiac plexus blocking surgery
    LI Cheng-li,WU Le-bin,SONG Ji-qing,LV Yu-bo,ZHANG Chuan-chen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1244)      PDF(pc) (577KB)(834)       Save
    Objective:To study the procedure of open MR-guided celiac plexus blocking surgery and to evaluate its effect. Methods: MR-guided celiac plexus blocking surgery was performed on 12 patients with late stage cancer due to severe chronic abdominal pain. Five patients accompanied with extensive retroperitoneal lymph nodes involving into masses were subjected to surgeries in which needle tips and chemotherapy particles were inserted into the lymph nodes. The degree of pain relief was evaluated before and after the procedure. Results: All patients were successfully localized, targeted and treated under MRI guidance. MRI displayed the ethanol distribution throughout the celiac plexus. Eleven patients felt obvious relief and only one experienced no effect at all. Conclusion: MR-guided placement of the needle is an accurate, safe and effective technique for celiac plexus blocking surgery.
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    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (2): 161-163.  
    Abstract758)      PDF(pc) (668KB)(826)       Save
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    Correlation of Angiotension Ⅱ and its type Ⅰreceptor gene polymorphisms with HBV-induced liver cirrhosis
    LI Hua,ZHAO You-an,LV Hui,WU Hong-lei,WANG Hong-bo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1136)      PDF(pc) (328KB)(824)       Save
    Objective: To investigate the correlation of polymorphisms of 1166 A/C of Angiotensin Ⅱ type Ⅰ receptor gene ( AT1R 1166A/C) and angiotensin Ⅱ with hereditary susceptibility and clinical phenotype of HBV-induced liver cirrhosis. Methods: Peripheral blood samples were collected from 102 patients with HBV-induced liver cirrhosis and 106 healthy donors. Polymorphisms of the 1166A/C of AT1R gene were determined by PCR-RFLP, and plasma concentrations of Angiotensin Ⅱ were measured by RIA. Results: Plasma concentrations of angiotensin Ⅱ of the liver cirrhosis group were significantly higher than those of the control group (P<0.05). Plasma concentrations of angiotensin Ⅱ were also significantly higher in patients with the Child-Pugh C and B grades than those in patients with the Child-Pugh A grade (P<0.01 and P<0.05, respectively). They were also higher in patients with severe esophageal varices and an ascites than those with light esophageal varices and without ascites (P<0.05). There was not a significant difference in genotype and allele frequency of AT1R 1166A/C between the liver cirrhosisgroup and the control group (P>0.05). Conclusion: Plasma concentration of angiotensin Ⅱ is related to the progression of liver cirrhosis and the 1166A/C polymorphism of the AT1R gene is not related to hereditary susceptibility or progression of liver cirrhosis.
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    Significance and expression of Survivin protein in non-Hodgkin's lymphoma
    LIU Xin,LI Ying,XU Gong-li,XU Hong-zhi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1012)      PDF(pc) (289KB)(817)       Save
    Objective: To explore the expression of Survivin in non-Hodgkin′s lymphoma cells with variable aggressiveness and its clinical significance. Methods: The SP immunohistochemistry staining method was used to detect the expression of Survivin. Results: The positive rate of Survivin in the reactive hyperplasia of the lymph node was lower than that in non-Hodgkin′s lymphoma. The positive rate of Survivin was related to the aggressiveness of non-Hodgkin′s lymphoma, but it was not related to the sex, age, clinical stage, body symptoms, immunotype and initial organ. Different Survivin expressions were found between different levels of LDH. High Survivin expression was associated with low survival stage. Conclusion: Survivin may play certain roles in the oncogenesis and the progression of non-Hodgkins lymphoma, and it has positive effects on the prognosis of non-Hodgkins lymphoma.
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    Neuronal migration and substance P expression induced by cardiac myocytes in co-cultures of nodose ganglion and cardiac myocytes
    LIU Hua-xiang,LIU Zhen,XING Yi,XING Zi-ying,WANG Huai-jing,LI Zhen-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1140)      PDF(pc) (623KB)(814)       Save
    Objective: To determine the effects of cardiac myocytes on nodose ganglial neuronal migration and substance P expression in co-cultures of nodose ganglion and cardiac myocytes of newborn Wistar rats. Methods: Neuromuscular co-cultures of nodose ganglion and cardiac myocytes were established. The living cells of the cultures were observed with an inverted contrast microscope at different culture ages. At 72 h and 96 h of culture, the cultures were examined by the Holmes-reduced silver stain technique to observe neuronal migration. An immunohistochemical staining method was used for investigating the expression of substance P (SP). Results: The number of migrated neurons increased in neuromuscular co-cultures compared with that in the cultures of nodose ganglial explants alone at 72 h and 96 h of culture (P<0.05). SP-immunoreactive neurons and nerve fibers were observed in the co-cultures at 96 h of culture age. However, in the neuromuscular co-cultures at 72 h of culture, and in the cultures of nodose ganglial explants alone at 72 h and 96 h of culture, no SP-immunoreactive neurons and nerve fibers were observed. Conclusion: Cardiac myocytes may have inductive effects on nodose ganglial neuronal migration and SP expression in neuromuscular co-cultures. The neuronal morphorlogical maturation cannot represent the maturation of neurotransmitter synthesis. The synthesis of neurotransmitters had not maturated in the neuromuscular co-cultures until 96 h of culture. The results provide a new method to study the selective innervation of cardiac myocytes and the interactions between neurons and cardiac myocytes.
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    Structural changes of myocardium sleeves in canine pulmonary veins due to sustained atrial fibrillation
    MA Xiao,ZHANG Wei,YANG Guirong,ZHONG Ming,LI Li,ZHANG Yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1103)      PDF(pc) (249KB)(807)       Save
    Objective: To study structure changes of myocardium sleeves in canine pulmonary veins(PVs) due to sustained atrial fibrillation(AF). Methods: The tissue and cell structure in individual veins was assessed by lightmicroscopy using HE and Masson′s Trichrome staining. Results: Compared with control dogs, sections from AF dogs showed cellular hypertrophy, disarranged cell bundles and increasing connective tissue. Some myocytes were surrounded by collagen tissue and myofilament was disrupted. Masson′s Trichrome stain showed that from the left atrium towards the vein, and the myocardial sleeves were gradually attenuated and ended in a rounded tip surrounded by fibrous tissue. Within myocardial sleeves, sharp transitions in fiber orientation were sometimes observed. It was apparent that myocytes on the endocardial side of myocardial sleeves were cut transversely in longitudinal sections. Samples from AF dogs showed, however, collagen tissue increased markedly and disrupted some areas. Conclusion: The special structure of the myocardial sleeve in PVs and its structural remodeling due to sustained AF may provide a substrate for forming micro reentry, which is an important mechanism for the occurrence and maintenance of AF.
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    Effects of anti-epileptic drugs on the blood levels of homocysteine, folate and vitaminB12
    WANG Xiao-hua,ZAHNG Tong-xia,ZHAO Xiu-he,Gao Jing,LIU Xue-wu,CHI Zhao-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract854)      PDF(pc) (243KB)(804)       Save
    Objective: To investigate the effect of antiepileptic drugs(AEDs) on the blood levels of homocysteine, folate and vitaminB12. Methods: There were 3 groups tested: ①88 epipleptic patients, taking a single AEDs; ②epileptic control group, 30 epileptic patients not taking AEDs; ③normal control group, 40 healthy controls. The levels of homocysteine, folate and vitaminB12 were determined by a fluorescence quantity method and an electrochemistry radiation immunity. Results: In epileptic patients taking a single carbamazepine (CBZ) and phenytoin(PHT), the levels of homocysteine were significantly higher and the levels of folate were significantly lower than those of the control group(P<0.001). The levels of homocysteine, folate and vitaminB12 were not significantly changed in patients taking valproic acid(VPA)(P>0.05). Conclusions: Homocysteine levels of epileptic patients taking CBZ and PHT were significantly increased whereas the folate levels of epileptic patients taking CBZ and PHT were signifacantly decreased. An adequate dosage of folate and vitaminB12 for epileptic patients is helpful in preventing angiocardiopathy and cerebrovascular diseases.
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    Role of the SPK/S1P signal in the expression of VEGF in human hepatoma cells
    JI Da-lei,GAO Yan-jing,FU Jin-dong,SONG Jian-hua,XU Jjian-min
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1199)      PDF(pc) (243KB)(801)       Save
    Objective:To investigate the role of the SPK/S1P(sphingosine kinase/sphingosine-1-phospate) signal in regulating the expression of the vascular endothelial growth factor(VEGF) in human hepatoma cells. Methods: The human hepatoma cell line HepG2 was cultured in vitro. After treatment with a concentration of 5-20μmol/L DMS (dimethyl sphingosine) in culture medium,the VEGF protein levels at 12h were measured by an enzyme-linked immunosorbent assay(ELISA). Results: The expression of VEGF was obviously inhibited by DMS in a dose-dependent manner. Conclusion: DMS makes the VEGF expression decrease by inhibiting the SPK/S1P signal, suggesting that the SPK/S1P signal regulates the expression of VEGF in hepatoma cells.
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    A multivariate and multilevel model in longitudinal data
    WANG Qi-wen,WANG Jie-zhen,XUE Fu-zhong,WANG Yan-mei,XU Ling-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract956)      PDF(pc) (211KB)(793)       Save
    Objective: To explore the applicability of a multivariate and multilevel model in a longitudinal research with several dependent variables. Methods: Influences of the independent variables (year, area and grade) on the two dependent variables (the total value of instruments and equipments in hospital and the operation income) and the relationship between the two dependent variables were assessed by the model. Results: Independent variables of year and hospital level, rather than area were associated with the increase of the two dependent variables. The two dependent variables positively correlated with each other. Conclusion: Multivariate and multilevel models can effectively analyze longitudinal data with several dependent variables.
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    Protection of edaravone against lung ischemia reperfusion injury in rabbits
    ZHOU Qing-ling,WU Jian-wen,FANG Yu-song,JIAO Wei,肖伟
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract996)      PDF(pc) (495KB)(787)       Save
    Objective: To investigate whether or not edaravone has an effect on reducing lung ischemia-reperfusion injury in rabbits. Methods: Twenty-four rabbits were randomly divided into three groups: ① the control group(group C, n=8): received sternotomy only and no ischemia; ② the ischemia/reperfusion group(group I/R, n=8): the left lungs of rabbits were rendered ischemia by ligating the left pulmonary hili for 60 minutes followed by 60 minutes reperfusion; ③ the edaravone group(group E, n=8): the left lungs were rendered ischemia for 60 minutes followed by 60 minutes reperfusion, and 10 mg/kg edaravone was administered intravenously 5 minutes prior to ischemia. Blood MDA and SOD were measured. Protein concentrations, WBC count, and PMN percentage in broncho-alveolar lavage fluid were determined. Samples of left lung tissue were sent for determining the left lung dry-to-wet weight ratio and evaluating the pathologic changes. Results: Blood MDA level, protein concentrations, WBC count and PMN percentage in bronchoalveolar lavage fluid of group I/R were significantly higher than those of group C and group E(P<0.01), and there was not significant difference between group C and group E (P>0.05); serum SOD activity, serum albumin concentration and left lung dry-to-wet ratio of of group I/R were significantly lower than those of group C and group E(P<0.05), and there was not significant difference between group C and group E (P>0.05); the pulmonary permeability index of group I/R was significantly higher than that of group C and group E(P<0.01); pathologic evaluations revealed mild alterations in alveolar structure, less white blood cell infiltration and less interstitial edema in group E than in group I/R. Conclusions: This study suggests that intravenous edaravone prior to ischemia attenuates the lung ischemia reperfusion injury in rabbits through its radical scavenging and antioxidant action.
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    Effect of long-term dietary protein feeding on glucose-stimulated insulin secretion in normal rats
    LI Ming-long,ZHAO Jia-jun,WANG Ming-yan,LIN Xin-ying,LIU Zhan-feng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1353)      PDF(pc) (263KB)(785)       Save
    Objective: To investigate the effects of long-term protein diet with normal total calorie intake on glucose-stimulated insulin secretion in normal rats. Methods: 51 healthy male Wistar rats (8 weeks old) were selected and divided into three groups: the routine feed group (NC, n=16), the high protein diet group (HP, n=19) and the low protein diet group (LP, n=16). The total calorie ingestion of each rat per day was similar and was used for 24 weeks. Body weight, fasting blood glucose (FBG) and fasting plasma insulin (FINS) were monitored during feedings. The insulin secretary function of β cells, especially the acute insulin response(AIR) were determined by the intravenous glucose tolerance test (IVGTT) in 32-week-old rats. Results: 1. Body weight and visceral fat mass: Compared with the NC group, there were no differences of the HP or LP groups. 2. IVGTT: (1) Blood glucose: There were no differences among the three groups. (2) Plasma insulin: 5min- and 10min- plasma insulin levels were markedly higher in the HP group than in the NC group[(6.5±0.8)ng/ml vs (4.9±0.7)ng/ml, P<0.001; (3.9±0.6)ng/ml vs (3.1±0.5)ng/ml, P<0.001], and there were no differences between the LP and NC groups. Conclusion: The long-term high protein ingestion with total calorie may significantly enhance the AIR by IVGTT in rats, while low protein intakes has not significant effect on insulin secretion in rats.
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    Nanoparticles of diallyl trisulfide inhibit the growth of orthotopic transplanted hepatocellular carcinoma
    ZHANG Zhi-mian,YANG Xiao-yun,DENG Shu-hai,XU Wei,SHI Cheng-yao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract836)      PDF(pc) (304KB)(784)       Save
    Objective: To study the antitumor effect and the relevant mechanism of the hepatic targeted polybutylcyanoacrylate nanoparticles of diallyl trisulfide (DATS-PBCA-NP) on orthotopic transplanted HepG2 hepatocellular carcinoma in nude mice. Methods: The orthotopic transplantation HCC models were established by implanting HCC HepG2 xenograft bits under the envelope of the mice liver. Then, they were divided into 4 groups: the normal saline (NS) group, the empty nanoparticles group, the diallyl trisulfide (DATS) group, and the DATS-PBCA-NP group. Two weeks later, the tumor volume was determined, the PCNA expression in cancer tissues was determined by using immunohistochemistry, and the apoptosis and cell proliferation were determined by Western blot. Results: The successful rate of tumor orthotopic transplantation was 100%. Intravenous administration of DATS-PBCA-NP significantly delayed the growth of orthotopic transplantation hepatoma in nude mice (compared with the other three groups, all P<0.05). The results of immunohistochemistry and western blot analysis showed that tumors from DATS-PBCA-NP-treated mice exhibited a markedly down-regulating expression of PCNA compared with the control tumor. There was no significant difference in the expression of Fas and FasL proteins among the four groups (P>0.05). Conclusion: DATS-PBCA-NP has a significant antitumor effect on the orthotopic transplantation HCC model in association with the suppression of proliferation tumor cells.
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    Health-related quality of life in patients with obstructive sleep apnea hypopnea syndrome
    ZHANG Xiao-wen,CAI Xiao-lan,PAN Xin-liang,LEI Da-peng,LIU Da-yu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract819)      PDF(pc) (267KB)(780)       Save
    Objective: To evaluate the health-related quality of life in patients with obstructive sleep apnea hypopnea syndrome and the influencing factors. Methods: The Calgary questionnaire for health-related quality of life of patients with sleep apnea and the Epworth sleepiness scale(ESS) were used to evaluate the quality of life and excessive daytime sleepiness(EDS) in OSAHS patients and simple snoring patients. The Pearson correlation and stepwise multiple regression analysis were used to analyze the correlation between the SAQLI and body mass index (BMI), neck collar, abdomen circumference, sleep architecture, respiratory disturbances and EDS. Results: There was a difference in each aspect between OSAHS patients and simple snoring patients. The SAQLI was significantly correlated with EDS. Conclusions: The health-related quality of life in patients with OSAHS is reduced. EDS is an important influencing factor for the health-related quality of life in patients with OSAHS.
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    SiRNA mediated silencing of the epidermal growth factor receptor induces the apoptosis of HepG2
    MOU Zhuang-bo,ZHAO You-an,WANG Yan-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1058)      PDF(pc) (350KB)(780)       Save
    Objective: To evaluate the RNAi efficiency of siRNA plasmid on the EGFR gene in a HepG2 cell line. Methods: By using a plasmid vector containing duplex of oligonucleotides, in which 19nt specific sequence of EGFR expressing cassette to induce RNAi, the expression of endogenous EGFR in HepG2 human hepatocarcinoma cells was suppressed. The HepG2 cells were transfected with pSIREN-hE consisting of a RNAi-ready pSIREN-shuttle vector and duplex of oligonucleotides, which were built up of 69nt base pair, into a 19nt specific sequence of EGFR expressing cassette that can be transcripted by promoter U6. Results: The mRNA of EGFR in HepG2 human hepatocarcinoma cells was decreased and cell proliferation was reduced due to the induction of apoptosis. Conclusion: siRNA-mediated inhibition of EGFR gene may be a useful approach in the treatment of human hepatocarcinoma.
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    Effects of valsartan and low molecular weight heparin on the growth of glomeruli mesangial cells stimulated by high glucose
    WANG Yun-shan,LIU Fang-e,JIAO Bo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1035)      PDF(pc) (201KB)(777)       Save
    Objective:To observe the effect of valsartan and low molecular weight heparin on the proliferation of glomeruli mesangial cells stimulated by high glucose. Methods:Mesangial cell line was treated by valsartan and low molecular weight heparin in different concentration and time, the proliferation activity was measured by MTT. Results:Both valsartan and low molecular weight heparin could inhibit the proliferation of mesangial cells significantly, and when combined the effect was intensified. Conclusion:The result suggests that the inhibition on mesangial cells is intensified when combined valsartan with low molecular weight heparin, compared with single dose.
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    An animal model of recurrent seizures in the neonatal rat
    SHI Xiu-yu, WANG Ji-wen,LI Xing-xia,SUN Ruo-peng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract891)      PDF(pc) (292KB)(772)       Save
    Objective: To establish a kind of rat model of recurrent neonatal seizures, and evaluate its morphological and behavioral effects. Methods: The rats in this study were divided into experimental group (n=20) and control group (n=12). Neonatal Wistar rats (n=20) of experimental group were subjected to three times of pilocarpine injections intraperitoneally at postnatal day 1, 4 and 7. Neonatal rats (n=12) of the control group were given saline injection intraperitoneally at the same time. All rats were administered bromodeoxyuridine (BrdU) 36 hours before killed to label new born cells. Observe the rats′ behavioral and histological changes at different times. Results: With the increase of seizure number, the mean time to onset and the dosage of pilocarpine decreased, and the manifestations of seizures became intenser. No seizure and death were found in the control group. BrdU-labeled cells decreased significantly in the experimental group comparing with the controls at P7 (P<0.05), while at P52 BrdU-labeled cells increased significantly in the experimental group (P<0.05). There was no evident neuron loss in both the experimental and the control groups at P7 and P52. Conclusion: The established recurrent seizures model neonatal rat is reliable for investigating the temporary and chronic effects of neonatal seizures.
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    Effect of rapid palatal expansion on AST activities in gingival crevicular fluid in adolescents
    ZHANG Yong,GUO Xin-xing,WANG Chun-ling,WEI Fu-lan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1182)      PDF(pc) (263KB)(768)       Save
    Objective: To study the relationship between aspartate aminotransferase (AST) activities and periodontal tissue remodeling during rapid palatal expansion in adolescents. Methods: Forty-three patients (aged 10.2-12.5 years) were divided into two groups: the control group(21 cases) and the experimental group(22 cases). GCF samples were collected with filter paper strips at 6 time points: before activation, 24 hours, the first week, the second week, the third week, and the fourth week after activation. AST activities were determined by full automatic biochemical analysis. The results were expressed as total AST activities (μU/30s). Results: AST activities were significantly elevated at 24 hours after activation by the Haas appliance, and remained at a high level until the fourth week after activation. Moreover, from 24 hour after activation to the fourth week after activation, AST activities in the experimental group significantly increased. Conclusion: The results suggest that AST activities in GCF reflect the tissues remodeling which occurs in the periodontium to some degree during rapid palatal expansion.
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    Primary study on the expression of bFGF and TGF-β in the posterior sclera of human fetuses with family-based myopia
    XIAO Ying,LI Jing-hai,ZHOU Ya-bin,GAO Zhi-juan,TANG Wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1213)      PDF(pc) (270KB)(761)       Save
    Objective: To evaluate the expression of basic fibroblast growth factor(bFGF) and transforming growth factor-beta(TGF-β) of the posterior sclera in human fetuses of the same month. Methods: Sixteen eyes of human 14-week fetus were divided into two groups: the experimental group whose parents were myopia with a refraction over -4.00D and the control group whose parents were without myopia. The expressions of bFGF and TGF-β in the posterior sclera were determined and quantitatively analyzed by immunohistochemistry and RT-PCR. Results: The expression of bFGF in the experimental group was significantly higher than that in the control group (P<0.05), while on the contrary, the expression of TGFβin the experimental group was significantly lower than that in the control group (P<0.05). Conclusion: The expressions of bFGF and TGF-β in the posterior sclera of the fetal eyes of the same fetal month are significantly different between myopia families and non-myopia families.
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    Application of the direct digital dental radiography system to diagnose proximal caries in premolars
    LIU Mei,WANG Duo,WANG Xu-xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract807)      PDF(pc) (237KB)(760)       Save
    Objective: To explore the clinical value of dental radiograph process in digital dental radiography system (DDRS)in diagnosing and treating the proximal caries. Methods: The patients, who were diagnosed as proximal caries with clinical exploration, were taken dental radiographs. The radiographs′ noises were processed and the radiographs were arranged in random order according to their computer number. One kind of image processing setup, following by another one assessed at least one week later, which could eliminate mutual obstruction, was examined jointly by three observers. Relative clinically diagnostic yields and four kinds of image processing setup were compared statistically. Results: Relative diagnostic accordance rate of the clinical exploration and four kinds of image processing setup was higher(P>0.01)in the first premolars. Relative diagnostic accordance rate of the clinical exploration and the pseudocolor image setup and negative image setup was higher(P>0.01), and that of the clinical exploration and the pseudo3D image setup and positive image setup was lower(P< 0.01), showing statistical differences in the second premolars. Conclusion: The computed image processing technique in DDRS can be applied to clinical examination of the proximal caries in premolars as an adjunct method.
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    Changes of hepatic alpha-SMA and TIMP-1 induced by interferon-alpha-2b combined with ribavirin in patients with chronic hepatitis C
    LIU Zhi-rong,WANG Lei,LU Hui,YU Shu-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract961)      PDF(pc) (269KB)(756)       Save
    Objective: To investigate the changes of liver tissue inhibitors metalloproteinases-1(TIMP-1) and alpha-smooth muscle actin (α-SMA) induced by alpha-interferon-2b plus ribavirin therapy in patients with chronic hepatitis C. Methods: A total of 23 patients were enrolled in this study. All patients had received a 48-week interferon-alpha-2b plus ribavirin and had undergone virological follow-up for 24 weeks. In each patient, two liver biopsies had been performed: 1 week before treatment and 2 weeks after treatment. The patients were divided into two groups according to the viral response on 24 weeks after treatment: sustained viral response (SVR) group and non-SVR group. α-SMA and TIMP-1 in liver tissue were examined by immunohistochemical techniques and BI-2000 image-analysis system. Results: Posttreatment liver α-SMA and TIMP-1 were significantly lower than pretreatment scores(P<0.05) in all patients. The groups between SVR and non-SVR were compatible in pretreatment results(P>0.05). Posttreatment liver α-SMA and TIMP-1 were also significantly lower than pretreatment (P<0.05) in SVR patients. But we did not found the same consequence in non-SVR patients(P>0.05). There was a statistical difference between SVR and non-SVR patients in the changes of α-SMA and TIMP-1(P<0.05). Conclusion: Interferon-alpha-2b plus ribavirin has the ability of anti-fibrosis by reducing the expression of liver α-SMA and TIMP-1. The liver fibrosis is improved significantly in patients receiving SVR, and no worsens in non-SVR patients.
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    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract649)      PDF(pc) (162KB)(754)       Save
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    Construction of recombinant hepatocytes-targeting retroviral vectors
    QI Peng,HAN Jing-xiang,LU Yan-qin,WANG Chuan-xi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1221)      PDF(pc) (712KB)(753)       Save
    Objective: To package five recombinant retroviral vectors containing the enhanced green fluorescent protein (EGFP) gene and screen for the best hepatocytes-targeting retroviral vector. Methods: Four additional expression plasmids were constructed based on the previously constructed fusion expression plasmid. Following a simultaneous transfection of five plasmids and plasmid pL-EGFP into NIH3T3 packaging cells respectively, which can stably express gag-pol protein, recombinant retroviral vectors were achieved 48h post-transfection. The virus supernatant was collected and used to infect HepG2215 and 293T cells. The total RNA of 48 h post-infected cells was extracted, and the tropism of the recombinant vectors were compared by real-time PCR. Results: Four additional expression plasmids were successfully constructed by enzyme digest identification. The viruses titer was about 105 cfu/ml, and the retrovirus stocks packaging with the plasmid pcDNA3.1(-)-envm-preS1-S2 had a better hepatocellular tropism. Conclusion: The recombinant retroviral vector which has a better hepatocellular tropism would provide great help for packaging the virus vector carrying the HDV ribozyme and studying the inhibition on HBV replication and expression induced by the HDV ribozyme.
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    Ursolic acid inhibits the angiogenesis of transplanted human lung adenocarcinoma cell line A549 in nude mice
    LI Xi-bo,SUN Xue-fei,YIN Qiu-wei,WANG De-jiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract970)      PDF(pc) (283KB)(752)       Save
    Objective: To study the inhibitory effect of ursolic acid on the transplanted human lung adenocarcinoma cell line A549 in nude mice and explore its mechanism. Methods: The nude mice bearing A549 cancer cells were established in vivo. The transplanted tumor growth curve was drawn, and the inhibitive ratio was calculated according to tumor weight. Immunohistochemistry (S-P)was used to examine the expression of VEGF and MVD of the transplanted tumor, and TUNEL (Terminal deoxynucleotidyl transferase mediated UTP nick end labeling) was used to examine the apoptosis index of the transplanted tumor. Results: The transplanted tumor of the treated group grew slowly. The volume and the weight of tumors in the treated group were smaller than those in the control group. The tumor inhibitive rate was 51.35%. The expressions of VEGF and MVD in the treated group were lower than those of the control group, and the apoptosis index of the transplanted tumor in the treated group was higher than that in the control group(P<0.01). Conclusion: Ursolic acid has an anti-angiogenesis activity on the human lung adenocarcinoma cell line A549 in vivo by inducing the apoptosis of the cancer cells, deceasing the VEGF expression and inhibiting the vasoformation of the transplanted tumor.
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    Differentiation of bla-TEM genes in highly resistant Acinetobacter lwoffi strains
    YU Xiu-juan,YAO Ping,KONG Qing-lian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1262)      PDF(pc) (239KB)(752)       Save
    Objective: To discover the extended spectrum beta-lactamases (ESBLs) gene types and the characteristics of Acinetinbacter lwoffi strains which were highly resistant to most kinds of antibiotics and to compare the differentiation in nucleotide and amino acid sequences to show the possible influence in laboratory. Methods: Three strains of A.lwoffi with ESBLs phenomenon were isolated from patients′ sputum suffering from respiratory infection. The genes of blaTEM, SHV, OXA, IMP and CTX-M were determined by PCR. Nucleotide sequences were detected by ABI automated sequencer and analyzed by the DNA star software to compare the differentiation in nucleotide and amino acid sequences. Results: The bla-TEM gene type was determined in two strains, but the blaTEM, SHV, OXA, IMP and CTX-M genes were not determined in another strain. Amplified nucleotide sequences of TEM genes were 1012bp and 1007bp, respectively. The identical rate was 98.2%. There were 17 pairs different in them. The blaTEM gene, 832bp, coded for 277 amino acids. Among them, 8 amino acids were different, which mainly happened on Asp, Gly and Ser, substituted by His, Arg/Ala and Cys. They had pI value of 5.479 and 5.083, respectively. Conclusion: The blaTEM gene presented in A.lwoffi highly resistant strains in Shandong province. However, they produce some differentiations in nucleotide and amino acid sequences. These differentiations influence some conditions of laboratory method to pick up the resistant strains.
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    Relationship between expression of P-AKT and angiogenesis of non-small cell lung cancer
    GAO Xi-gang, WANG Ling
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1042)      PDF(pc) (525KB)(751)       Save
    Objective: To investigate the levels of microvessel density(MVD), the expression of P-AKT and vascular endothelial growth factor(VEGF) in non-small cell lung cancer (NSCLC), and explore the correlation between them and the clinicopathological features of NSCLC. Methods: MVD and expressions of P-AKT and VEGF in 46 specimens of NSCLCS tissues and 18 specimens of normal lung tissues were detected by SP immunohistochemistry. Their interrelations and correlations were analyzed. Results: Positive rate of P-AKT and VEGF were significantly higher in NSCLC than in normal lung tissues (43.5% vs 0% and 67.4% vs 22.2%, P<0.05); there were correlations between PAKT expression and lymph node metastasis, between the TNM stage and differentiation of NSCLC(P<0.05) and between VEGF expression and lymph node metastasis and the TNM stage of NSCLC(P<0.05). The MVD of lung cancer tissues was higher than that of normal lung tissues(24.62±6.21 vs13.26±5.96, P<0.05); there were correlations between the MVD and lymph node metastasis and between the TNM stage and differentiation of NSCLC(P<0.05). There were positive correlations between P-AKT expression and VEGF expression and MVD(P<0.05). Conclusion: P-AKT is over-expressed in NSCLC, which correlates with lymph node metastasis, the TNM stage and differentiation of NSCLC and takes part in the angiogenesis of NSCLC.
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    Different VX2 liver tumor in rabbits and its image features
    FENG Yan, LI Cai-xia, LI Chun-hai,NING Dan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract945)      PDF(pc) (288KB)(750)       Save
    Objective: To evaluate the success rate of VX2 tumor in rabbits and study the image manifestations by different methods. Methods: VX2 tumor was implanted in 60 New Zealand rabbits by three methods: laparotomic burying, laparotomic puncture and percutaneous puncture, 20 rabbits per group. Ten were subjected to carcinoma masses and ten carcinoma fluids in each group. CT, MRI and liver angiography were performed two weeks after inoculation. Results: Implantation rate was significantly higher in the two laparotomic routes compared with the percutaneous route. The VX2 liver tumors were demonstrated as hypodensity on plain CT scanning and enhanced at an early artery phase and hypodensity at a portal phase image. On MR scannings, the VX2 liver tumors were seen as hypointensity on T1WI and slight hyperintensity on T2WI. Hepatic angiography showed the tumors were hypervascular and the hepatic artery was the main feeding vessel. Necrosis and bleeding were often found in the area of VX2 liver tumors two weeks after implantation. Conclusion: Construction of VX2 liver tumor by a laparotomic route is simple and short circled. The image manifestations of the VX2 liver tumor in rabbits are similar to those of hepatic primary carcinoma in human.
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    Evaluation the short-term effects of uvulopalatopharyngoplasty on the sleep apnea quality of life index
    SHI Lei,LI Yan-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract778)      PDF(pc) (284KB)(749)       Save
    Objective: To evaluate the significance of the sleep apnea quality of life index (SAQLI) for uvulopalatopharyngoplasty (UPPP) and quality of life in patients with obstruction sleep apnea hypopnea syndrome(OSAHS). Methods: Thirty patients with OSAHS were diagnosed by PSG and treated by UPPP. SAQLI was surveyed before and after surgery. Results: Before operation, the score of SAQLI was greatly reduced and had significant statistically correlation with the apnea hypopnea index(AHI) (r=-0.54) and lowest saturation (LSaO2) (r=0.56). After operation, the total score of SAQLI including 4 domains increased significantly (P<0.001). Quality of life was improved in 86.7% cases in general. By SAQLI, we could discriminate among those with different qualities of life. Conclusion: The amelioration of quality of life in OSAHS patients is significant in the short term after UPPP. SAQLI can be a valuable measurement in clinical diagnosis and follow-up treatment.
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    GONG Hui,ZHANG Yan-li,ZHUANG Gui-ying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract795)      PDF(pc) (139KB)(747)       Save
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    LI Zhen-feng,LI Jian-min,YANG Zhi-ping,LI Xin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract974)      PDF(pc) (335KB)(746)       Save
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    Effect of all trans-retinoic acid on the proliferation and apoptosis of human Tenon's capsule fibroblasts in vitro
    DAI Li-hua,LI Wei-ning,QIAO Zhi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract905)      PDF(pc) (278KB)(746)       Save
    Objective: To study the effect of all trans-retinoic acid (ATRA) on the proliferation and apoptosis of human Tenon′s capsule fibroblasts(HTCFs) in vitro. Methods: Cell cycle distribution, the apoptotic rate and the expression of bcl-2 were determined by flow cytometry. Cell DNA was determined by agarose gel electrophoresis. Mitomycin C was used as a positive control. Results: Cell cycle distribution of HTCFs showed that cells of the G0/G1 phase increased and of the S phase decreased after the treatment with ATRA and MMC. Cellular apoptosis was decreased by ATRA at a lower dose (1×10-9mol/L), and the apoptotic rate was significantly increased in a dose-dependent manner. Expression of bcl-2 was significantly decreased by ATRA. A “ladder” strand of DNA was produced of the ATRA groups by DNA agarose gel electrophoresis. Conclusion: ATRA can significantly inhibit the proliferation of HTCFs and induce the apoptosis of HTCFs without apparent cytotoxicity.
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    D2-40 positive micro-lymphatic vessel density in the thyroid papillary carcinoma correlate with lymphatic metastasis
    LIU Hong,ZHANG Jian-ping,ZHAO Zhu-mei,WANG Yan,LIU Wen-jun,ZHANG Ze-li,LI Hong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1197)      PDF(pc) (461KB)(744)       Save
    Objective: Specimens of papillary thyroid carcinoma and nodular goiter have been immunohistochemically studied by using a novel monoclonal antibody D2-40 which is specific against lymph epithelial cells in the detection of the newly formed micro-lymphatic vessel density(MLVD) in lesions in order to seek clues of papillary thyroid carcinoma and its well known ability to involve regional lymph nodes. Micro-vascular density(MVD)as the future of tumor angiogenesis was immunochemically compared by CD34. Methods: MLVD and MVD were determined by conventional HE staining and Envison immunohistochemistry using D2-40 and CD34 monoclonal antibodies in 46 specimens of papillary thyroid carcinoma and 20 of nodular goiter. Results: The micro-lymphatic vessel of papillary thyroid carcinoma in the peripheral zone of stroma appeared to be associated with the spread of tumors to regional lymph nodes. Conclusion: The metastasis process of thyroid papillary carcinoma is associated with the formation of the micro-lymphatic vessel and micrangium. The antimetastatic potential for targeting these lymphatics may be of potential therapeutic benefit in the future.
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    Expression of HIF-1α and P-gp and their correlation in patients with hepatocellular carcinoma
    YU Fang,ZHOU Geng-yin,ZHANG Cui-juan,GAO Peng,MA Chao,LI Hong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1183)      PDF(pc) (354KB)(741)       Save
    Objective: To study the expression of hypoxia induced factor-1α (HIF-1α) and P-gp and their correlation in patients with hepatocellular carcinoma (HCC). Methods: Immunohistochemistry was used to detect the expression of HIF-1α and P-gp in thirty-two HCC samples and twenty noncancerous liver samples. If the expressions of both HIF-1α and P-gp were positive in the same case, another slide was used to detect the co-expression of the two. Results: The positive expressions of HIF-1α (58.33%) and P-gp (77.78%) in HCC were significantly higher than those in the non-cancerous group (P<0.001). The expression of HIF-1α was positively correlated with the expression of P-gp (r=0.388, P<0.05). The co-expression rate was 61.1%. Conclusion: The expression of HIF-1α and P-gp has a close correlation in HCC, and HIF-1α may induce multi-drug resistance by up-regulating the expression of P-gp, and it may become a novel marker to evaluate and reverse multi-drug resistance.
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    Expression of NT3 and trkC in the hippocampus of Alzheimer disease rats induced by amyloid β-peptide25-35
    DAI Ting-jun,SHAN Pei-yan,WANG Shu-hua,GAO Jing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1331)      PDF(pc) (1010KB)(733)       Save
    Objective: To demonstrate the changes in the expression of NT3 and trkC in the hippocampal neurons of Alzheimer disease (AD) rats induced by amyloid β-peptide25-35 (Aβ25-35) and to investigate the mechanism of NT3 and trkC involved in the pathogenesis of AD. Methods:Thirty male Wistar rats were randomly divided into control and model groups. The AD models were established by the injection of amyloid β-peptide25-35 (Aβ25-35) into the bilateral hippocampus of rats, while the control rats were subjected to injection of normal saline at the same site. All were tested with the Morris water maze for memory and learning function two weeks after injection. After that, they were killed at the same time. The hippocampal microstructures were observed by an optic microscope. The expressions of NT3 and trkC were detected by an immunohistochemical method. Results: Different expression levels were presented between NT3 and trkC in the different subfields of the hippocampus. The number of NT3 imunoreactive neurons within the hippocampal subfields CA1, CA2 and CA3 in the model group was obviously reduced compared with the control group (P< 0.01), while no difference was found in the CA4 and hilar subfields between the two groups (P>0.05). No difference in the number of trkC imunoreactive neurons in each hippocampal subfield was produced between the two groups (P>0.05). Conclusion: The decrease of the expression of NT3 but not the down-regulation of its receptor′s in the hippocampus is related to learning and memory dysfunction of AD rats, suggesting an exogenous NT3-supply may counteract the neurotoxicity of β amyloid protein.
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    Expression of CD15S antigen on peripheral lymphocytes before and after acute rejection following renal transplantation
    MENG Hui-lin,LIU Yu-qiang,LV Jia-ju,LIU Jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract873)      PDF(pc) (296KB)(732)       Save
    Objective: To investigate the difference of CD15S antigen expression on peripheral lymphocytes and its clinical significance in patients before and after acute rejection and of those with a stable graft function after renal transplantation. Methods: Flow cytometry was used to detect the expression of CD15S antigen on peripheral lymphocytes in 70 patients( 95 specimens). These subjects were divided into 3 groups: patients with a stable graft function (group 1), patients with acute rejection (group 2) and patients recovered after an anti-rejection therapy (group 3). There were respectively 45, 25 and 25 subjects in each group. The percent of CD15S positive lymphocytes of each subject was detected. CD15S expression of every group was compared with a Student t test. Result: The data of group 2 were significantly higher than those of the other 2 groups, and there were no significant differences between group 1 and 3. Conclusions: CD15S expression of group 3 is equal to that of group 1. Monitoring of CD15S antigen expression on peripheral lymphocytes after renal transplantation may contribute to the diagnosis of acute rejection and may predict the effect of an anti-rejection therapy.
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