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    Determination of the Benzo(a)pyrene level in cooking oil by solid
    phase extractionhigh performance liquid chromatography
    YANG Yan, FU Ya-qun, WU Xiao-meng, JIANG Qing-qing, SHAO Li-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (8): 124-127.  
    Abstract1486)      PDF(pc) (1287KB)(121644)       Save

    Objective   To establish a high performance liquid chromatography with fluorescence detector for the determination of the benzo(a)pyrene(B(a)P) level in the cooking oil. Methods   The difference of B(a)P content in edible oil in Jinan market was investigated by the SHIMADZU shim-pack VP-ODS (250mm×4.6mm, 5μm) column. The mobile phase was methanol-water(90∶10, V/V ), and the flow rate was 1. 0mL/min. The samples were treated by the non-bonded silica solid phase extraction(SPE) column, and then detected at the 285nm excitation wavelength and 406nm emission wavelength. Results   The lowest limit of determination was 0.039μg/kg (S/N=3). The recovery rate was 85.8%-90.7%,  and the intra-day and inter-day relative standard deviations were 2.9%-4.1% and 3.7%-5.3%. Conclusion   This method is high sensitive, good selective and quantitative accurate, which is suitable for the B(a)P determination in the cooking oil.

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    Rosuvastatin reverses the effect of heavy drinking on angiogenesis after infarction in rats
    QU Fuchao, WANG Aihong, SUN Yongle, WANG Yong, YUAN Haitao, ZHANG Yuying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    DOI: 10.6040/j.issn.1671-7554.0.2013.552
    Heterogeneity of human peripheral and decidual monocytes of women in early pregnancy
    ZHANG Yun 1,2, SUN Jin-tang 2, HUANG Xian-liang 3, YANG Mei-xiang 2,SHAO Qian-qian 2, DENG Bi-ping 2, XU Xiao-fei 3,MAO Hai-ting 2, DONG Ba
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (3): 98-101.  
    Abstract1375)      PDF(pc) (444KB)(2888)       Save

    Objective    To analyze the distribution of human CD14+CD16- and CD14+CD16+ monocyte subsets in peripheral blood and decidua of women in early pregnancy. Methods    Peripheral blood samples were collected from early pregnant and nonpregnant females. Mononuclear cells were isolated from the fresh decidua of early pregnant females by mechanical trituration and Ficoll gradient centrifugation. The fluorescent intensity of the cells was detected by FCM. Results    The percentage of the peripheral CD14+CD16- monocyte subtype in early pregnant females was lower than that in  non-pregnant controls(P<0.01). CD14+CD16+ cells (69.13%±7.73%)  and were the major monocyte subset in the decidua of early pregnant females. Conclusion    CD14+CD16+ monocytes become the predominant cell subpopulation of women in early pregnancy. It has been demonstrated that CD14+CD16+ monocyte-derived dendritic cells are efficient producers of anti-inflammatory interleukin-10, so CD14+CD16+ monocytes  might play an important role in forming and keeping the dominance of TH2 cytokine during early pregnancy.

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    Mucus fills the intestinal epithelial gaps and maintains the barrier function
    ZHOU Xiaoyan, LI Ming, GUO Jing, ZUO Xiuli, LI Yanqing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2015, 53 (12): 62-66,80.   DOI: 10.6040/j.issn.1671-7554.0.2015.148
    Abstract710)      PDF(pc) (3773KB)(1997)       Save
    Objective To investigate whether mucus fills the intestinal epithelial gaps(IEG), and to explore the role of mucus in maintaining local barrier function. Methods The terminal ileum tissues of ulcerative colitis (UC) patients and controls were obtained. Mucins were stained with Alcian Blue-Periodic Acid Schiff (AB-PAS) and Muc2. In addition, we also analyzed the confocal laser endomicroscopy (CLE) imagines of the terminal ileum from 39 UC patients and 34 controls since January 2013 to December 2014. Revised Watson grading system was used to evaluate the local barrier function. Results In UC patients, the proportion[M(P25,P75),%] of IEG without mucus, intestinal epithelial gaps, goblet cells were 27.52(25.00,29.68), 5.33(3.48,6.62) and 17.38(16.00,19.87), respectively. In control group, these three parameters were 12.27(9.78,16.67), 0.27(0.00,0.69) and 23.44(20.24,26.18), respectively. The differences were statistically significant in the two groups (P<0.01). The proportion of intestinal epithelial gaps and goblet cells showed a significantly negative correlation(r=-0.74, P<0.01). Conclusion The local barrier function is lost in UC patients; mucus filled with IEG play a key role to maintain the local intestinal barrier function.
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    A general overview of neuromodulation
    Di ZHANG,Meng YU,Xia LIU
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 50-60.   DOI: 10.6040/j.issn.1671-7554.0.2020.0602
    Abstract4027)   HTML154)    PDF(pc) (4269KB)(1346)       Save

    Neuromodulation refers to a series of biomedical engineering techniques which normalize or modulate the function of the nervous system through invasive or non-invasive delivery of physical or chemical stimulus. In the past thirty years, with the in-depth understanding of the nervous system and its mechanisms of action, as well as the rapidly developing modern techniques, neuromodulation has evolved from basic concept to clinical applications. At the same time, new concepts and alternative methods of neuromodulation are constantly being innovated, tested and put into practice. The advancing of neuromodulation techniques will not only provide new tools for basic neuroscience research but also offer novel therapeutic options for neurological and psychiatric disorders. In this overview, we will briefly introduce the current status of different types of neuromodulation techniques, including the clinical applications, underlying mechanisms of their therapeutic effects and advantages/disadvantages, as well as the prospects for the future.

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    Effect of dexmedetomidine on general anesthesia in
    hypertensive patients undergoing craniectomy
    ZHOU Ting-fa1, TANG Zhen-shen2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (8): 81-.  
    Abstract1008)      PDF(pc) (1001KB)(1270)       Save

    Objective   To detect the effect of dexmedetomidine(DEX) on general anesthesia of hypertensive patients undergoing retromastoid craniectomy for microvascular decompression(MVD) of facial nerve. Methods   48 (ASA Ⅰ-Ⅱ) hemifacial spasm patients with hypertension undergoing retromastoid craniectomy for MVD of facial nerve under general anesthesia were randomly divided into two groups: the study group and the control group. Dexmedetomidine was injected with the load dosage (1μg/kg) before anesthesia, followed by the maintaining dosage [0.20.7μg/(kg·h)] during anesthesia in the study group. Sodium chloride injection was pumped in the control group. Anesthesia was maintained with propofol and remifentanil. During the surgery, systolic blood pressure (SBP) was maintained 70%-80% of the baseline value by titration of anesthetic drugs and/or nitroglycerin in both groups. The mean infusion rates of propofol and remifentanil, anesthesia recovery and extubation times were observed. Results   Compared with the control group, patients in the study group required lower normalized infusion rates of propofol [(0.076±0.014)mg/(kg·min) vs (0.092 ± 0.018)mg/(kg·min), P<0.05] and remifentanil [(0.106±0.032) vs (0.131±0.030)μg/(kg·min), P<0.05]. Anesthesia recovery times [(5.2±2.1)min vs (9.3±2.4)min] and extubation times [(5.3±2.4)min vs (10.3±3.2)min] were also significantly shorter than those in the control group (P< 0.05). Conclusion   Dexmedetomidine can effectively reduce the consumption of anesthetic drugs, shorten the recovery  times and hasten the extubation in the hypertensive patients during retromastoid craniectomy undergeneral anesthesia.

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    Contents and ratios of type I and type III collagens in normal skin and hypertrophic scar in people of different ages
    WANG Cheng, RONG Yanhua, SHEN Yuming, ZHANG Guoan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2016, 54 (11): 64-67.   DOI: 10.6040/j.issn.1671-7554.0.2015.1043
    Abstract2778)      PDF(pc) (1915KB)(1191)       Save
    Objective To quantify the contents of type I and III collagens and their ratios in normal skin and hypertrophic scar in people of different ages, and to explore the rule and cause of changes. Methods Normal human skin specimens and hypertrophic scar specimens were obtained from spontaneously aborted fetus and people of different ages, including the children and adolescent group(≤18 years), adult group(19-50 years), elderly group(>50 years). The total collagen contents were determined by hydroxyproline method. The ratios of types I and III collagens were examined by immunohistochemistry, and their contents were calculated. Results The difference of the contents of types I and III collagens and their ratios in normal skin were statistically significant among different age groups(P=0.01). Their ratios increased with the increase of age, and the differences were statistically significant among different age groups(P=0.02). The difference of contents of types I and III collagens and their ratios in hypertrophic scars were statistically significant among all groups, also between any two groups(P=0.03). In every age group, the difference of the contents of types I and III collagens and their ratios between normal skin and hypertrophic scar was statistically significant (P=0.02). Conclusion With age increasing, the changes of the ratios of types I and III collagens in the normal skin changes, which results in changes of the tension and elasticity of skin and the healing process after injury. The contents of types I and III collagens and their ratios significantly changes in hypertrophic scars, resulting in different structure of scar from normal skin.
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    Effect of double-lumen-tube or bronchial blocker used for one-lung ventilation on PetCO2 and PaCO2
    YANG Xiaomei, FANG Ningning, YI Wenbo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2015, 53 (11): 77-79.   DOI: 10.6040/j.issn.1671-7554.0.2015.262
    Abstract934)      PDF(pc) (1526KB)(1172)       Save
    Objective To assess the effect of double-lumen-tube (DLT) and bronchial blocker for one-lung ventilation on partial pressure of end-tidal carbon dioxide (PetCO2) and arterial partial pressure of carbon dioxide (PaCO2). Methods Thirty patients were randomly assigned to DLT group (n=15) and BB group (n=15). After anesthesia induction, DLT or BB was intubated into the trachea. After one-lung ventilation, the values of PetCO2 and PaCO2 were recorded and analyzed. Results Compared with BB group, the PetCO2 value was higher, the PaCO2 value was lower, and the correlation between PetCO2 and PaCO2 was better in DLT group. Conclusion Compared with BB, DLT was better at exhalation of CO2.
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    Signal quality assessment of ECG in the intensive care unit
    LI Qiao,YU Meng-sun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1414)      PDF(pc) (250KB)(1163)       Save
    Objective: To develop an algorithm of signal quality assessment of ECG in the intensive care unit. Methods: The signal quality index (SQI) was obtained by analyzing the morphological and statistical characteristics of each waveform and their relationships. The heart rate was robustly estimated based on SQI. The algorithm was evaluated using more than 6?000 hours of simultaneously acquired ECG from a 437patient subset of the MultiParameter Intelligent Monitoring for Intensive Care II database and adding real ECG noises. Results: The SQI was decreased along with a decrease of the signal noise ratio. The SQI had high-positive correlation with the QRS detective sensitivity and positive predictivity. This method provided an accurate HR estimation even in the presence of high levels of persistent noises and artifacts. Conclusion: The signal quality assessment algorithm could provide an objective estimation of ECG quality.
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    Current status and prospect of viral vaccines research and development
    Kai WANG
    Journal of Shandong University (Health Sciences)    2021, 59 (5): 8-14.   DOI: 10.6040/j.issn.1671-7554.0.2021.0273
    Abstract2829)   HTML167)    PDF(pc) (1285KB)(1153)       Save

    Virus infection can cause several diseases in human. In the past decades, outbreaks and epidemics of respiratory infectious diseases caused by coronary viruses, especially coronavirus disease 2019 (COVID-19), have become serious health hazards and posed huge challenges to public health. The development and application of viral vaccines have been an important means to counteract the spread of viruses. Historically, poliovirus and hepatitis B virus vaccines have successfully suppressed the transmission of diseases and provided a model for the development of vaccines. Recently, the emergence of novel technologies and platforms such as recombinant viral vector and mRNA vaccine has provided new approaches to the development of vaccines against emerging infectious diseases including COVID-19. This paper reviews the current situation and progress of platforms, summarizes problems to be solved, and forecasts the directions of viral vaccines development.

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    Expression and signficance of TNF-related apoptosis inducing ligand and death receptor-5 in atherosclerosis rabbits
    FEI Ling,SUI Shu-jian,REN Man-yi,XU Fu-yu,LIU Wei-hua,DU Yi-meng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1256)      PDF(pc) (422KB)(1074)       Save
    Objective: To explore whether TNF-related apoptosis inducing ligand(TRAIL)and death receptor-5(DR5) take part in the occurrence and development of atherosclerosis(AS). Methods: Twenty rabbits were randomly distributed into two groups: the control group and the atherosclerotic group. The experimental atherosclerotic models were made by endothelial injury on the ventro-aorta with a balloon and hyperlipid feeding lasting 12 weeks. The levels of sTRAIL and sDR5 in the serum were determined by enzyme linked immunoabsorbent assay (ELISA). The proteinum expression of TRAIL and DR5 in the ventro-aortas was detected by immunohistochemical technique. Results: In the course of research, 2 rabbits died. The survival rate was 90%. Till the end of the research, the serum sDR5 in the AS group was more significantly elevated than that in control group (P<0.05), while the serum sTRAIL in two groups had no significant differences (P>0.05). The expressions of TRAIL and DR5 in the ventro-aortas in the AS group were significantly elevated than those in the control group (P<0.05). Conclusion: This study suggests that TRAIL and DR5 could promote the occurrence and development of AS. TRAIL and DR5 may be important factors for AS.
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    Effect of polypeptide extract from scorption venom on tumor growth and cellular immunity in rats with W256 sarcocarcinoma
    ZHANG Wei-dong,ZHANG Yue-ying,WANG Zhao-xia,WANG Zhao-peng,JIA Qin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1766)      PDF(pc) (267KB)(1068)       Save
    Objective: To study the inhibitory mechanism of polypeptide extract from scorpion venom (PESV) on W256 sarcocarcinoma and its influence on T subsets in rats. Methods: Tumor models were established by subcutaneously implanting W256 tumor cells into thirty Wistar rats. The tumor-bearing rats were randomly divided into three groups: the model group, the 5-fluorouracil(5-FU) group, and the PESV group. PESV was intragastrically subjected to the rats of the experimental group for 7 days. Then, the tumor-bearing rats were killed, and T subsets in the peripheral blood were determined by flow cytometry (FCM). The presence of interferon-gamma (INF-γ) and interleukin-4 (IL-4) were screened by an enzyme-linked immunosorbent assay (ELISA). Results: PESV had an inhibitory effect on tumor growth, and the growth inhibitory rate was 55.2%. The number of CD4+T and the CD4+T/CD8+T ratio of W256-bearing rats treated with PESV significantly increased compared with the model group (P<0.05). The expression level of interferon-gamma (INF-γ) was up-regulated and of interleukin-4 (IL-4) down-regulated. Compared with the control group, the difference was significant(P<0.05). Conclusion: PESV has an inhibitory effect on tumor growth by strengthening the immunocellular function. It could be regarded as a candidate of tumor inhibition.
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    Actovegin restores the digestive tract in rats with acute radiation enteritis
    BI Ying-hui,HAN Jun-qing,SHENG Wei,LI Zhao-pei,HU Ping-ping,SHEN Bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1770)      PDF(pc) (817KB)(1044)       Save
    Objective: To evaluate the effect of Actovegin in restoring the digestive tract in rats with acute radiation enteritis. Methods: Whole abdominal radiation was performed on Wistar rats. The rats were randomly divided into three groups: the Actovegin group (Actovegin injection), the control group(irradiation only), and the normal group (without irradiation). Morphologic indices were measured by using light microscopy and the image analysis system. Intestinal NO concentration was detected by nitric acid reductase and bacterial translocation was also examined. Results: The Actovegin group had a significantly higher level of the height of the villus, the depth of crypt, the thickness of the mucosa and the entire wall, the number of the metaphase mitoses per crypt, and a significantly lower level of bacterial translocation and NO concentration compared with the control group. Conclusion: Actovegin accelerates recovery of injured digestive mucosal epithelium and decreases intestinal NO concentration and is good for regaining normal structure and function of digestive mucosa.
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    Effects of anti-epileptic drugs on the blood levels of homocysteine, folate and vitaminB12
    WANG Xiao-hua,ZAHNG Tong-xia,ZHAO Xiu-he,Gao Jing,LIU Xue-wu,CHI Zhao-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1411)      PDF(pc) (243KB)(1043)       Save
    Objective: To investigate the effect of antiepileptic drugs(AEDs) on the blood levels of homocysteine, folate and vitaminB12. Methods: There were 3 groups tested: ①88 epipleptic patients, taking a single AEDs; ②epileptic control group, 30 epileptic patients not taking AEDs; ③normal control group, 40 healthy controls. The levels of homocysteine, folate and vitaminB12 were determined by a fluorescence quantity method and an electrochemistry radiation immunity. Results: In epileptic patients taking a single carbamazepine (CBZ) and phenytoin(PHT), the levels of homocysteine were significantly higher and the levels of folate were significantly lower than those of the control group(P<0.001). The levels of homocysteine, folate and vitaminB12 were not significantly changed in patients taking valproic acid(VPA)(P>0.05). Conclusions: Homocysteine levels of epileptic patients taking CBZ and PHT were significantly increased whereas the folate levels of epileptic patients taking CBZ and PHT were signifacantly decreased. An adequate dosage of folate and vitaminB12 for epileptic patients is helpful in preventing angiocardiopathy and cerebrovascular diseases.
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    Significance of ETV6 rearrangement in acute myelogenous leukemia-M4Eo
    GAO Na,LI Zhi-hong,DING Bu-tong,CHEN Yun,WANG Yun-shan,GUO Nong-jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1275)      PDF(pc) (312KB)(1036)       Save
    To explore the clinical significance of ETV6 gene rearrangement involved in acute myelogenous leukemia M4Eo. Methods3 cases of AML -M4Eo were prepared the chromosome after the bone marrow cells culture. R-banding technique was used to analyze the karotypes. 12p13 translocation was determined by Split-signal FISH in the interphase and metaphase cells. ETV6/ARG fusion genes were analyzed by RT-PCR. ResultsClone chromosomal abnormalities with inv(16) was found by Karotype analysis in 3 cases, abnormal fluorescence signal with 12p13 translocation was found in 1 case by split-signal FISH, and ETV6/ARG fusion gene was found by RT-PCR. This was the second report that ETV6/ARG expressed in AML-M4Eo. ConclusionThis patient is not sensitive to chemical therapy, even has worse prognosis than patient without this fusion gene. Splitsignal FISH is a reliable measure to detect ETV6 gene rearrangement.
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    Hemostatic effect of hemocoagulase used in bladder irrigation and
    intravesical instillation after transurethral resection of the prostate
    DING Sen-tai1, ZHAO Peng1, L Jia-ju1, XING Nai-dong1, NIU Zhi-hong1, SUN Hui2, WU Hai-hu1, SUN Ding-qi1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (10): 93-96.  
    Abstract1751)      PDF(pc) (959KB)(1028)       Save

    Objective   To evaluate the hemostatic effect of hemocoagulase used in bladder irrigation and intravesical instillation after transurethral resection of the prostate (TURP). Methods   From March 2010 to March 2012, 69 selected patients who underwent TURP were randomly divided into three groups, and all patients were administrated with postoperative continuous bladder irrigation using  NS conventionally. 20 cases in the intravesical instillation group were given postoperative intravesical instillation using 6U hemocoagulase in 60mL NS for 20min, bid for 3d. 23 cases in the bladder irrigation group were given bladder irrigation using 10U hemocoagulase in 100mLNS, bid for 3d. The rest of 26 patients were assigned to the control group with continuous bladder irrigation using normal saline (NS). The bleeding volumes in the 3 days after surgery of the 3 groups were recorded and analyzed by using SPSS16.0 software and the coagulation function and adverse effects were observed. Results   There was no significant difference of patients′ age, prostate volume or operation time among the three groups, additionally, no obvious coagulant function change and complications occurred in the patients of the three groups. Compared with that of the control group, the total bleeding volumes of the postoperative 3 days in the experimental groups of hemocoagulase were significantly reduced (P<0.05). However, for 3 days′ total or each day′s blood bleeding volume, no significant difference was observed between the bladder irrigation group and the intravesical instillation group. Conclusion   Local application of hemocoagulase was effective and safe in reducing postoperative bleeding after TURP.

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    NIU Fu-wen,NIE Lin,HOU Yong,CHENG Lei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1318)      PDF(pc) (183KB)(1019)       Save
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    Relationship between expression of P-AKT and angiogenesis of non-small cell lung cancer
    GAO Xi-gang, WANG Ling
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract2014)      PDF(pc) (525KB)(1008)       Save
    Objective: To investigate the levels of microvessel density(MVD), the expression of P-AKT and vascular endothelial growth factor(VEGF) in non-small cell lung cancer (NSCLC), and explore the correlation between them and the clinicopathological features of NSCLC. Methods: MVD and expressions of P-AKT and VEGF in 46 specimens of NSCLCS tissues and 18 specimens of normal lung tissues were detected by SP immunohistochemistry. Their interrelations and correlations were analyzed. Results: Positive rate of P-AKT and VEGF were significantly higher in NSCLC than in normal lung tissues (43.5% vs 0% and 67.4% vs 22.2%, P<0.05); there were correlations between PAKT expression and lymph node metastasis, between the TNM stage and differentiation of NSCLC(P<0.05) and between VEGF expression and lymph node metastasis and the TNM stage of NSCLC(P<0.05). The MVD of lung cancer tissues was higher than that of normal lung tissues(24.62±6.21 vs13.26±5.96, P<0.05); there were correlations between the MVD and lymph node metastasis and between the TNM stage and differentiation of NSCLC(P<0.05). There were positive correlations between P-AKT expression and VEGF expression and MVD(P<0.05). Conclusion: P-AKT is over-expressed in NSCLC, which correlates with lymph node metastasis, the TNM stage and differentiation of NSCLC and takes part in the angiogenesis of NSCLC.
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    Role of iodine on wounds in the skin of rats
    CHEN Li-xin,WANG Yi-bing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1496)      PDF(pc) (749KB)(979)       Save
    Objective: To study the effect of iodine on wounds in the skin of rats. Methods: A bilateral round full-thickness wound was made on the back of Wistar rats. The benefit effect of iodine on the skin wound was evaluated by determining closure time and contracting ability of the skin wound. The expression of VEGF in the wound tissues was determined by immunohistochemical techniques and an image analysis system. Results: The closure time of skin wounds was significantly shorter in the 10HI-treated groups than that in the sodium chloride-treated groups. The percentage of the wound contraction was higher in the 10HI-treated groups than that in the sodium chloride-treated groups (P<0.01 and P<0.05) between the 1st and the 7th day after wounding. There was no difference between the 1000HI-treated groups and the sodium chloride-treated groups. Histopathological results showed that 10HI could promote the formation of new born capillaries and the proliferation of fibroblasts at an early period of wound healing and promote the expression of VEGF from fibroblasts and the vascular endothelial cells; whereas 1000HI reversed these. Conclusion: Iodine of benefit concentration has the ability to promote healing in the skin of rats, but iodine of high concentration can delay healing.
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    Tumescent technique in multi-position liposuction
    CAI Jing-long,ZHU Gui-ying,DONG Hong,JIANG Du-yin,XU Bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1219)      PDF(pc) (344KB)(969)       Save
    Objective: To study the clinical effect of tumescent technique in multi-position liposuction. Methods: Tumescent liposuction technique was used on 1200 different sites of 500 cases. The pre- and post-operative treatment, the tumescent liquid amount, the usage of microcannula in the superficial fat layer, the lidocaine dosage, instruments and operation methods, and the maintenance of body fluid equilibrium were all well controlled. Results: There was good efficiency such as fewer complications, obviously shrinking skins and smooth operation areas. Conclusion: Tumescent technique can meet the need of multi-position and large-volume liposuction. It is a safe and effective body sculpture technique.
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    Different VX2 liver tumor in rabbits and its image features
    FENG Yan, LI Cai-xia, LI Chun-hai,NING Dan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1521)      PDF(pc) (288KB)(968)       Save
    Objective: To evaluate the success rate of VX2 tumor in rabbits and study the image manifestations by different methods. Methods: VX2 tumor was implanted in 60 New Zealand rabbits by three methods: laparotomic burying, laparotomic puncture and percutaneous puncture, 20 rabbits per group. Ten were subjected to carcinoma masses and ten carcinoma fluids in each group. CT, MRI and liver angiography were performed two weeks after inoculation. Results: Implantation rate was significantly higher in the two laparotomic routes compared with the percutaneous route. The VX2 liver tumors were demonstrated as hypodensity on plain CT scanning and enhanced at an early artery phase and hypodensity at a portal phase image. On MR scannings, the VX2 liver tumors were seen as hypointensity on T1WI and slight hyperintensity on T2WI. Hepatic angiography showed the tumors were hypervascular and the hepatic artery was the main feeding vessel. Necrosis and bleeding were often found in the area of VX2 liver tumors two weeks after implantation. Conclusion: Construction of VX2 liver tumor by a laparotomic route is simple and short circled. The image manifestations of the VX2 liver tumor in rabbits are similar to those of hepatic primary carcinoma in human.
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    Assessing the general mental health of college students:psychometric properties of GHQ-28
    CHEN Cui, ZHANG Hongjing, JIANG Hong, LI Wenjie, Lv Li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (3): 159-162.  
    Abstract8312)      PDF(pc) (330KB)(967)       Save

    Objective   To check the psychometric properties of GHQ-28 in college students. Methods   Three questionnaires, GHQ-28, SAS, and SDS, were used to assess 248 college students. Then the item-analysis was used to analyze each item of the GHQ-28,  the Cronbach′s α of the GHQ-28 was checked,  factor analysis was used to check the structure validity of the GHQ-28 and the SAS and SDS was applied to check the criterion validity of the GHQ-28. Results    The correlations between each item of the GHQ-28 and its scales were positive (P<0.01). The reliabilities of the whole scale and the part scales were 0.872, 0.767, 0.667, 0.756 and 0.700. The GHQ28 contains four factors, and the aberration rate of them was 48.381% of the total. The GHQ-28 factors′ levels of students with high scores in SAS were higher than those low in SAS, as well as in SDS (P<0.001). The correlations of the GHQ-28 and SAS, SDS were 0.707 and 0.749. Conclusion    The GHQ-28 has good reliability and validity when it measures the general mental health of college students.

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    Research advances in the formation of glioma immunosuppressive microenvironment
    Gang LI,Hao XUE,Wei QIU,Rongrong ZHAO
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 67-73.   DOI: 10.6040/j.issn.1671-7554.0.2020.430
    Abstract1574)   HTML34)    PDF(pc) (1393KB)(963)       Save

    As the most common and lethal primary tumor of the central nervous system, glioma responds poorly to conventional treatments. Although novel immunotherapies have been adopted, the prognosis of glioma patients is still poor due to the immunosuppressive microenvironment. Therefore, a thorough investigation of the formation mechanism of glioma immunosuppressive microenvironment has become the focus. Based on the latest research progress, we summarized and prospected the future research direction of glioma immunosuppressive microenvironment, hoping to provide reference for the better understanding of this disease.

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    Effect of long-term ethanol feeding on glycogen and structures of the livers of Wistar rats
    CHEN Shao-hua,ZHAO Jia-jun,FENG Li,HOU Xiao-lei,ZHANG Wei,YU Hui,GAO Ling
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1369)      PDF(pc) (509KB)(957)       Save
    ObjectiveTo investigate the effects of a long-term ethanol feeding on the glycogen content and structure of the liver in rats. Methods48 rats were randomly divided into 4 groups: the ethanol groups A, B and C and the control group. Rats were subjected to ethanol of 0.5, 2.5, and 5g/(kg·d) in the experimental groups respectively and saline in the control group for 5 months. Different physical parameters, HE staining, PAS staining and the glycogen content( Colorimetry, SpectraMax M2,) were determined. ResultsCompared with group D, groups A, B and C showed a lower weight of body and liver. Glycogen levels of groups A, B and C decreased, and the glycogen decreased in an ethanol dosedependent manner. HE staining showed lots of lipid vacuoles and inflammatory cells in groups A, B and C relative to group D. On the other hand, PAS staining revealed a decrease of glycogen granules. ConclusionLong-term intake of ethanol influences the glycogen content and causes some damage to the structure of liver tissues.
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    Role of the SPK/S1P signal in the expression of VEGF in human hepatoma cells
    JI Da-lei,GAO Yan-jing,FU Jin-dong,SONG Jian-hua,XU Jjian-min
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1570)      PDF(pc) (243KB)(951)       Save
    Objective:To investigate the role of the SPK/S1P(sphingosine kinase/sphingosine-1-phospate) signal in regulating the expression of the vascular endothelial growth factor(VEGF) in human hepatoma cells. Methods: The human hepatoma cell line HepG2 was cultured in vitro. After treatment with a concentration of 5-20μmol/L DMS (dimethyl sphingosine) in culture medium,the VEGF protein levels at 12h were measured by an enzyme-linked immunosorbent assay(ELISA). Results: The expression of VEGF was obviously inhibited by DMS in a dose-dependent manner. Conclusion: DMS makes the VEGF expression decrease by inhibiting the SPK/S1P signal, suggesting that the SPK/S1P signal regulates the expression of VEGF in hepatoma cells.
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    Significance and expression of Survivin protein in non-Hodgkin's lymphoma
    LIU Xin,LI Ying,XU Gong-li,XU Hong-zhi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1350)      PDF(pc) (289KB)(951)       Save
    Objective: To explore the expression of Survivin in non-Hodgkin′s lymphoma cells with variable aggressiveness and its clinical significance. Methods: The SP immunohistochemistry staining method was used to detect the expression of Survivin. Results: The positive rate of Survivin in the reactive hyperplasia of the lymph node was lower than that in non-Hodgkin′s lymphoma. The positive rate of Survivin was related to the aggressiveness of non-Hodgkin′s lymphoma, but it was not related to the sex, age, clinical stage, body symptoms, immunotype and initial organ. Different Survivin expressions were found between different levels of LDH. High Survivin expression was associated with low survival stage. Conclusion: Survivin may play certain roles in the oncogenesis and the progression of non-Hodgkins lymphoma, and it has positive effects on the prognosis of non-Hodgkins lymphoma.
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    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1271)      PDF(pc) (221KB)(933)       Save
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    Detection and significance of CD4+CD25+ Foxp3+ regulatory T cells in peripheral blood of patients with vitiligo
    LI Qiang1,2, CAI Da-xing1, CHEN Guang3, LIU Ji-feng4, LI Chun-yang1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (1): 133-.  
    Abstract906)      PDF(pc) (3172KB)(920)       Save

    Objectives   To detect CD4+CD25+Foxp3+ regulatory T cells in peripheral blood of patients with vitiligo, and to evaluate immunomodulator′s therapeutic effects. Methods   36 patients with vitiligo were selected, including 24 with active vitiligo and 12 with stationary vitiligo. The level of CD4+CD25+Foxp3+ regulatory T cells in peripheral blood was detected by flow cytometry before and after treatment with immunomodulator, 20 healthy subjects as controls. Results   CD4+CD25+Foxp3+ regulatory T cells in active vitiligo were significantly lower than in normal controls(P<0.05).  There was no statistical difference between stationary vitiligo and normal controls(P>0.05). CD4+CD25+Foxp3+ regulatory T cells in active vitiligo increased after comprehensive treatment with immunomodulator(P<0.05); while there was no statistical difference in stationary vitiligo after treatment with immunomodulator(P>0.05). Conclusion   CD4+CD25+Foxp3+ regulatory T cells could control the autoimmune reaction by inhibiting autoreactive T cells. The abnormal number of CD4+CD25+ Foxp3+ regulatory T cells in peripheral blood may cause imbalance of immunity. Polysaccharide nucleic acid fraction of bacillus calmette guerin(BCGPSN) could modulate the cell immune function by regulating the level of CD4+CD25+ Foxp3+ regulatory T cells and plays therapeutic effects.

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    Effect of gypenosides on proliferation of neural precursor cells in vitro
    XIE Shanshan1, WANG Yuzhuo1, SUN Tao1, ZHANG Daolai2, FENG Yuxin1, XIN Hua1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (2): 85-90.  
    Abstract1312)      PDF(pc) (973KB)(919)       Save

    Objective  To study the effect of gypenosides (GPs) on proliferation of neural precursor cells (NPCs) in vitro. Methods  NPCs were isolated from the brains of embryonic rats on day14 of pregnancy. After adherent culture for 7 days, the cells were passaged for the first time, and cultured for another 3 days. We identified the purity of the NPCs by immunofluorescence technique, then incubated the NPCs together with GPs in different concentrations(0, 25, 50, 100, 200 and 400μg/mL)for 48 hours. After that the purity of the NPCs was again identified,  activity was measured by MTT chromatometry, a cell growth curve was drawn by cell counting,  the proliferation of NPCs was measured by bromodeoxyuridine (BrdU) incorporation, and determined the expression level of proliferating cell nuclear antigen (PCNA) was determined by Western blot. Results  The purity of the NPCs cultured for 3 days after being passaged for the first time was 97 %. Without changing the purity of NPCs, GPs increased the activity of NPCs, accelerated the growth of NPCs, improved the positivity rate of BrdU, and up-regulated the expression level of PCNA. Conclusion  GPs promote the proliferation of NPCs in vitro through increasing the expression level of PCNA, and the optimal concentration of GPs is 100μg/mL.

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    Open MR-guided celiac plexus blocking surgery
    LI Cheng-li,WU Le-bin,SONG Ji-qing,LV Yu-bo,ZHANG Chuan-chen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1637)      PDF(pc) (577KB)(915)       Save
    Objective:To study the procedure of open MR-guided celiac plexus blocking surgery and to evaluate its effect. Methods: MR-guided celiac plexus blocking surgery was performed on 12 patients with late stage cancer due to severe chronic abdominal pain. Five patients accompanied with extensive retroperitoneal lymph nodes involving into masses were subjected to surgeries in which needle tips and chemotherapy particles were inserted into the lymph nodes. The degree of pain relief was evaluated before and after the procedure. Results: All patients were successfully localized, targeted and treated under MRI guidance. MRI displayed the ethanol distribution throughout the celiac plexus. Eleven patients felt obvious relief and only one experienced no effect at all. Conclusion: MR-guided placement of the needle is an accurate, safe and effective technique for celiac plexus blocking surgery.
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    A multivariate and multilevel model in longitudinal data
    WANG Qi-wen,WANG Jie-zhen,XUE Fu-zhong,WANG Yan-mei,XU Ling-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1383)      PDF(pc) (211KB)(914)       Save
    Objective: To explore the applicability of a multivariate and multilevel model in a longitudinal research with several dependent variables. Methods: Influences of the independent variables (year, area and grade) on the two dependent variables (the total value of instruments and equipments in hospital and the operation income) and the relationship between the two dependent variables were assessed by the model. Results: Independent variables of year and hospital level, rather than area were associated with the increase of the two dependent variables. The two dependent variables positively correlated with each other. Conclusion: Multivariate and multilevel models can effectively analyze longitudinal data with several dependent variables.
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    SARS-CoV-2 infection, innate immunity and inflammatory response
    Xi ZHOU,Muhan HUANG,Yujie REN,Yang QIU
    Journal of Shandong University (Health Sciences)    2021, 59 (5): 15-21.   DOI: 10.6040/j.issn.1671-7554.0.2021.0368
    Abstract3110)   HTML188)    PDF(pc) (1512KB)(913)       Save

    Coronavirus disease 2019 (COVID-19), which is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has become the worst pandemic and public health crisis across the globe once a century. This pandemic has caused huge losses in both human lives and global economy. Innate immunity is the first line of defense against pathogenic invasions. Extensive studies by scientists in China and the world have reported that SARS-CoV-2 can employ multiple strategies to evade host innate immunity, and such immune evasion mechanisms have become critical contributing factors for the pathogenicity of SARS-CoV-2. On the other hand, the pathogenesis of COVID-19 has been found to be closely relevant with the pro-inflammatory responses induced by SARS-CoV-2 infection in humans. This paper provides a brief review to the relationship between SARS-CoV-2 infection and innate immunity as well as inflammation.

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    Health-related quality of life in patients with obstructive sleep apnea hypopnea syndrome
    ZHANG Xiao-wen,CAI Xiao-lan,PAN Xin-liang,LEI Da-peng,LIU Da-yu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1247)      PDF(pc) (267KB)(902)       Save
    Objective: To evaluate the health-related quality of life in patients with obstructive sleep apnea hypopnea syndrome and the influencing factors. Methods: The Calgary questionnaire for health-related quality of life of patients with sleep apnea and the Epworth sleepiness scale(ESS) were used to evaluate the quality of life and excessive daytime sleepiness(EDS) in OSAHS patients and simple snoring patients. The Pearson correlation and stepwise multiple regression analysis were used to analyze the correlation between the SAQLI and body mass index (BMI), neck collar, abdomen circumference, sleep architecture, respiratory disturbances and EDS. Results: There was a difference in each aspect between OSAHS patients and simple snoring patients. The SAQLI was significantly correlated with EDS. Conclusions: The health-related quality of life in patients with OSAHS is reduced. EDS is an important influencing factor for the health-related quality of life in patients with OSAHS.
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    Transcranial ultrasound-enhanced thrombolysis by urokinase for acute cerebral infarction in rats
    XIE Zhao-hong,ZHU Zheng-yu,YANG Hong-xia,BI Jian-zhong,LAI Chao,CONG Yan-bin,WANG Xiao-yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1569)      PDF(pc) (660KB)(897)       Save
    Objective: To observe the effect of transcranial ultrasound-enhanced thrombolysis by urokinase on acute cerebral infarction in rats. Methods: The animal model of acute cerebral infarction was made by injecting auto-thrombsis into the left middle cerebral artery. Intravenous urokinase and transcranial ultrasound were given at the same time. The functioning of the nervous system was measured by NSS. The infarct volume was measured after TTC staining. Pathological changes were observed by a light microscope and transmission electron microscope. Results: The NSS score of the large dose urokinase group and the ultrasound plus small dose urokinase group at 24 hours after treatment was lower than that before treatment(P<0.01) and of that of the infarct group(P<0.01). The infarct volume of the two groups was lower than that of the infarct group, but there were no differences between the two groups(P>0.05). Remission of pathology was seen under both light microscope and transmission electron microscope. Conclusions: Transcranial ultrasound enhanced thrombolysis by urokinase can accelerate the recovery of the nervous system in acute cerebral infarction rats, diminish the infarct volume, decrease the ischemic changes of the neurons, and reduce the dosage of urokinase.
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    Associations of maternal methionine synthase A2756G and methionine synthase reductase A66G polymorphisms with neural tube defects of the offspring: A meta-analysis
    ZHANG Bing-zhen, DAI Bing-qin, ZHANG Xing-liang, WANG Zhi-ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (7): 125-132.  
    Abstract1073)      PDF(pc) (3609KB)(891)       Save

    Objective   To evaluate associations of maternal methionine synthase (MTR) A2756G and methionine synthase reductase(MTRR) A66G polymorphisms with the offspring′s risk for neural tube defects (NTDs). Methods   The retrieval strategy and criteria for inclusion and exclusion were made. Literature was identified by searches for case-control studies on maternal MTR A2756G or MTRR G66A polymorphism and risk of NTDs in the offspring published in English or Chinese from following databases: China Biology Medical Literature Database(CBM), Database of Chinese Scientific and Technical Periodicals(VIP), China National Knowledge Infrastructure(CNKI), Digital Journal Full-text Database(CHINAINFO), PubMed and Web of Science from January 1990 to October 2010.Combined OR values and 95% CI were calculated with Review Manager 5.0. Results   18 eligible studies were included in the metaanalysis, among which 11 studies(907 cases and 1,978 controls) on maternal MTR A2756G polymorphism, and 11 (1123 casesand 1700 controls) on maternal MTRR A66G polymorphism. Statistical analysis of the combined data showed that there was no significant association between maternal MTR A2756G polymorphism and the risk of NTDs, while there were significant associations between maternal MTRR A66G polymorphism and the risk of NTDs in GG/AG vs. AA, GG vs. AA, AG vs. AA, GG vs. AG/AA and G vs. A genetic models, and their pooled OR values and 95% CI were 1.89(1.282.78), 1.68(1.312.16), 1.77(1.18-2.66), 1.28(1.06-1.55) and 1.35(1.12-1.63), respectively. Conclusion   Maternal MTRR A66G polymorphism is a risk factor for NTDs.

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    Effects on endothelial cell proliferation induced by different concentration of iodine in vitro
    WANG Xiao-lei,XU Li-ya,ZHANG Hai-tao,LI Jia-mei,GUO Cheng-hao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1545)      PDF(pc) (300KB)(888)       Save
    Objective: To research the effects on proliferation of human umbilical vein endothelial cells (HUVECs) induced by different doses of iodine. Methods: HUVECs were subjected to iodine in various concentrations (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 8.0μmol/L)for 24 hours, and the morphology of the cultured endothelium was observed. MTT colorimetry was used to determine the proliferation activities of the cultured endothelium. Results: Iodine significantly increased the number and MTT activities of vascular endothelial cells from 0.5μmol/L to 4.0μmol/L compared with the normal group at 24 hours. Cell morphology was normal under a microscope. The number and MTT activities of vascular endothelial cells were significantly increased by iodine at over 5.0μmol/L at 8 hours, but were decreased at 12 hours and later. Poison granules in the endothelium were produced by iodine at over 5.0μmol/L at 4 hours and increased in a dose-and time-manner. Conclusion: Iodine effects on the proliferation activity of endothelial cells are dose-and time-dependent.
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    Role of SPINK1 in prostate cancer: advances in experimental and
    translational research
    HAN Bo1,3, YANG Xiao-qing1, ZHANG Jing2, LIU Wen-jun3, LIU Long3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (8): 40-.  
    Abstract850)      PDF(pc) (6528KB)(886)       Save

    Altered genes that play a driving role in cancer development can often serve as specific diagnostic markers, criteria of molecular classification and therefore potential therapeutic targets. Serine protease inhibitor Kazal type 1 (SPINK1), also known as the pancreatic secretory trypsin inhibitor (PSTI) or the tumor-associated trypsin inhibitor (TATI), encodes a 56 amino acid secreted peptide, and its normal function is thought to be the inhibition of serine proteases such as trypsin. Recent studies have indicated SPINK1 may act as an autocrine growth factor and promote prostate cancer growth and invasion. The association between SPINK1 expression and adverse prognosis in prostate cancer has been reported. Notably, SPINK1 might be a novel extracellular therapeutic target in a subset of high-grade prostate cancers. Our preliminary data also suggested that overexpression of SPINK1 was significantly correlated with poor prognosis of prostate cancer patients in China. In this review, our group  will summarize the current understanding of SPINK1 in experimental and translational research of prostate cancer. Its potential applications in prognostic validation and therapeutic target selection for prostate cancer will be highlighted.

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    Brain tumor image segmentation based on deep learning techniques
    Ju LIU,Qiang WU,Luyue YU,Fengming LIN
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 42-49, 73.   DOI: 10.6040/j.issn.1671-7554.0.2020.0391
    Abstract2428)   HTML108)    PDF(pc) (6300KB)(885)       Save

    Artificial intelligence technology is widely applied in the field of computer vision and deep learning. Image segmentation technology based on deep learning is essential in industries such as autonomous driving, drones, and clinical diagnosis and treatment. This paper reviews the methods of brain tumor image segmentation, including the traditional methods of image segmentation and methods based on deep learning and some typical methods. The paper also compares our research advances with the typical methods and discusses future research directions and challenges.

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    Resistin serum levels in different groups and related factors
    XIAO Zheng-da,ZHANG Rong-hua,FEI Hong-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1255)      PDF(pc) (263KB)(885)       Save
    Objective To investigate the role of resistin in obesity and type 2 diabetes mellitus. Methods 55 patients with type 2 diabetes mellitus and 34 healthy controls were randomly enrolled in this study. No dysfunctions of heart, liver or kidney or other endocrine diseases were found. Based on plasma glucose and BMI, they were divided into four groups: the normal control group, the obese control group, the nonobese T2DM group, and the obese T2DM group. The levels of serum resistin, HbAlc, bloodfat, fasting plama glucose, and fasting insulin were determined. The insulin resistance index was calculated based on the HOMA model(HOMAIRI=FINS×FPG/225). Results The serum level of resistin of the obese T2DM group [(1402±468)ng/mL] was significantly higher than that of the other three groups (P<0001). The serum resistin levels of the obese group[(814±284)ng/mL] and the nonobese T2DM group[(856±502)ng/mL] were significantly higher than that of the normal control group[(562±156)ng/mL] (P<005, P<001). There were positive correlations between the level of serum resistin and the levels of HbA1c, LDL, IRI, SBP and BMI(r=0222, r=0233, r=0286, r=0407,  r=0480, respectively), and negative correlations between serum resistin and HDL and FINS (r=-0198, r=-0138, respectively). Stepwise regression analysis showed that BMI, HbA1c and systolic blood pressure highly influenced the serum level of resistin. Conclusion Resistin is an important factor linking obesity and type 2 diabetes mellitus. It may play a role in insulin resistance and obesity. Resistin is not only related to the metabolism of blood glucose and lipids, but also related to hypertension.
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    Nanoparticles of diallyl trisulfide inhibit the growth of orthotopic transplanted hepatocellular carcinoma
    ZHANG Zhi-mian,YANG Xiao-yun,DENG Shu-hai,XU Wei,SHI Cheng-yao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1151)      PDF(pc) (304KB)(883)       Save
    Objective: To study the antitumor effect and the relevant mechanism of the hepatic targeted polybutylcyanoacrylate nanoparticles of diallyl trisulfide (DATS-PBCA-NP) on orthotopic transplanted HepG2 hepatocellular carcinoma in nude mice. Methods: The orthotopic transplantation HCC models were established by implanting HCC HepG2 xenograft bits under the envelope of the mice liver. Then, they were divided into 4 groups: the normal saline (NS) group, the empty nanoparticles group, the diallyl trisulfide (DATS) group, and the DATS-PBCA-NP group. Two weeks later, the tumor volume was determined, the PCNA expression in cancer tissues was determined by using immunohistochemistry, and the apoptosis and cell proliferation were determined by Western blot. Results: The successful rate of tumor orthotopic transplantation was 100%. Intravenous administration of DATS-PBCA-NP significantly delayed the growth of orthotopic transplantation hepatoma in nude mice (compared with the other three groups, all P<0.05). The results of immunohistochemistry and western blot analysis showed that tumors from DATS-PBCA-NP-treated mice exhibited a markedly down-regulating expression of PCNA compared with the control tumor. There was no significant difference in the expression of Fas and FasL proteins among the four groups (P>0.05). Conclusion: DATS-PBCA-NP has a significant antitumor effect on the orthotopic transplantation HCC model in association with the suppression of proliferation tumor cells.
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    Package and expression of lentivirus encoding human GCSF genes
    LIN A-li,SUN Qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1332)      PDF(pc) (262KB)(878)       Save
    Objective: To construct a lentiviral vector encoding human granulocytemacrophage colony stimulating factor(hGM-CSF) genes and to transfect CT26 cells in order to establish the CT26 strains which has been continuously overexpressing the hGM-CSF gene. Methods: DNA fragments of human GM-CSF were amplified by PCR and were inserted into the vector plasmid L166. The three plasmids expressing lentivirus were packaged into virus packaging cell line 293T using the CaCl2 method. After 72 hours of transfection, the virusproducing cell supernatant was produced. CT26 cells were transfected by lentivirus and the titration was determined. Results: The sequence of the PCR product in the recombinant plasmid was produced. The three plasmids were effectively transferred into 293T. When the CT26 cells were infected as target cells by the lentivirus supernatant, the titration of lentivirus was 4.69×105IU/ml. hGM-CSF was determined in the supernatant of CT26 cells transfected by lentivirus, which was kept for more than two months. The transfected CT26 cells were screened by puromycin at a terminal concentration of 15μg/ml. Conclusion: The lentivirus encoding hGMCSF genes were successfully constructed.
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    An animal model of recurrent seizures in the neonatal rat
    SHI Xiu-yu, WANG Ji-wen,LI Xing-xia,SUN Ruo-peng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1204)      PDF(pc) (292KB)(876)       Save
    Objective: To establish a kind of rat model of recurrent neonatal seizures, and evaluate its morphological and behavioral effects. Methods: The rats in this study were divided into experimental group (n=20) and control group (n=12). Neonatal Wistar rats (n=20) of experimental group were subjected to three times of pilocarpine injections intraperitoneally at postnatal day 1, 4 and 7. Neonatal rats (n=12) of the control group were given saline injection intraperitoneally at the same time. All rats were administered bromodeoxyuridine (BrdU) 36 hours before killed to label new born cells. Observe the rats′ behavioral and histological changes at different times. Results: With the increase of seizure number, the mean time to onset and the dosage of pilocarpine decreased, and the manifestations of seizures became intenser. No seizure and death were found in the control group. BrdU-labeled cells decreased significantly in the experimental group comparing with the controls at P7 (P<0.05), while at P52 BrdU-labeled cells increased significantly in the experimental group (P<0.05). There was no evident neuron loss in both the experimental and the control groups at P7 and P52. Conclusion: The established recurrent seizures model neonatal rat is reliable for investigating the temporary and chronic effects of neonatal seizures.
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    Correlation of Angiotension Ⅱ and its type Ⅰreceptor gene polymorphisms with HBV-induced liver cirrhosis
    LI Hua,ZHAO You-an,LV Hui,WU Hong-lei,WANG Hong-bo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1440)      PDF(pc) (328KB)(875)       Save
    Objective: To investigate the correlation of polymorphisms of 1166 A/C of Angiotensin Ⅱ type Ⅰ receptor gene ( AT1R 1166A/C) and angiotensin Ⅱ with hereditary susceptibility and clinical phenotype of HBV-induced liver cirrhosis. Methods: Peripheral blood samples were collected from 102 patients with HBV-induced liver cirrhosis and 106 healthy donors. Polymorphisms of the 1166A/C of AT1R gene were determined by PCR-RFLP, and plasma concentrations of Angiotensin Ⅱ were measured by RIA. Results: Plasma concentrations of angiotensin Ⅱ of the liver cirrhosis group were significantly higher than those of the control group (P<0.05). Plasma concentrations of angiotensin Ⅱ were also significantly higher in patients with the Child-Pugh C and B grades than those in patients with the Child-Pugh A grade (P<0.01 and P<0.05, respectively). They were also higher in patients with severe esophageal varices and an ascites than those with light esophageal varices and without ascites (P<0.05). There was not a significant difference in genotype and allele frequency of AT1R 1166A/C between the liver cirrhosisgroup and the control group (P>0.05). Conclusion: Plasma concentration of angiotensin Ⅱ is related to the progression of liver cirrhosis and the 1166A/C polymorphism of the AT1R gene is not related to hereditary susceptibility or progression of liver cirrhosis.
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    Anti-oxidative effect of grape seed extract in the human body
    FENG Dian-juan,XU Gui-fa
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1385)      PDF(pc) (242KB)(875)       Save
    Objective: To study the anti-oxidative effect of grape seed extract (GSE) in human body. Methods: One hundred healthy volunteers aging from 45 to 65 were randomly divided into two groups: the control group and the GSE-treated group based on the SOD, GSH-Px and MDA levels in serum. The GSE capsule was administered to the GSEtreated group 1g/d orally for 3 months, while no drugs were administered to the control group. The SOD, GSH-Px and MDA levels in serum were determined both prior to and after the experiment. Results: The SOD and GSH-Px levels were significantly higher in the GSE-treated group than in the control group (P<0.05), while the MDA level was significantly lower in the GSE-treated group than in the control group (P<0.01). Moreover, there were significant differences in the three indexes in the GSE-treated group between prior to and after the experiment (P<0.01). Conclusion: GSE has a significantly anti-oxidative effect in vivo.
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    SiRNA mediated silencing of the epidermal growth factor receptor induces the apoptosis of HepG2
    MOU Zhuang-bo,ZHAO You-an,WANG Yan-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1337)      PDF(pc) (350KB)(874)       Save
    Objective: To evaluate the RNAi efficiency of siRNA plasmid on the EGFR gene in a HepG2 cell line. Methods: By using a plasmid vector containing duplex of oligonucleotides, in which 19nt specific sequence of EGFR expressing cassette to induce RNAi, the expression of endogenous EGFR in HepG2 human hepatocarcinoma cells was suppressed. The HepG2 cells were transfected with pSIREN-hE consisting of a RNAi-ready pSIREN-shuttle vector and duplex of oligonucleotides, which were built up of 69nt base pair, into a 19nt specific sequence of EGFR expressing cassette that can be transcripted by promoter U6. Results: The mRNA of EGFR in HepG2 human hepatocarcinoma cells was decreased and cell proliferation was reduced due to the induction of apoptosis. Conclusion: siRNA-mediated inhibition of EGFR gene may be a useful approach in the treatment of human hepatocarcinoma.
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    Expression of MMP-9 and PCNA in sinonasal inverted papilloma and the clinical significance
    LIU He,ZOU Xue,LI Min,WU Xiao-juan,ZHANG Li-qiang,YE Ping,LI Xue-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1399)      PDF(pc) (509KB)(870)       Save
    Objective: To explore the function of MMP-9 and PCNA in the occurrence, recurrence and malignancy of the sinonasal inverted papilloma (SNIP). Methods: Forty specimens with SNIP and ten specimens with squamous cell carcinoma (SCC) were selected for this study. All the specimens were divided into four groups: the SNIP group, the recurrent SNIP group, the SNIP with carcinoma group, and the invasive squamous cell carcinoma (SCC) group. The expression of MMP-9 and PCNA was determined by using an immunohistochemistry method. The results were analyzed by a quantitative immunohistochemical analysis. Results: Expression of increased in the SNIP group, the recurrent SNIP group, the SNIP with carcinoma group, and the SCC group. According to the rank test, the expression of MMP-9 was significantly different between the SNIP group and other groups. But between recurrent SNIP and SNIP with carcinoma, recurrent SNIP and SCC, SNIP with carcinoma and SCC, there were no prominent differences. The expression of PCNA was not significantly different between any two groups. Conclusion: The expression of MMP-9 and PCNA is involved in the mechanism of the occurrence of SNIP and plays an important role in the recurrence and malignancy of SNIP. The over expression of MMP-9 and PCNA may be associated with early events in the recurrence tendency and carcinogenesis/transformation to SNIP.
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    Role of pre-operative cerebral angiography in cerebral artery stenosis stent angioplasty
    WANG Zhi-ang,DING Xua,SUN Peng/sup>,WANG Cheng-wei,HAO Xiao-guang,PAN Shun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1181)      PDF(pc) (259KB)(864)       Save
    Objective: To study the value of pre-operative cerebral angiography in cerebral artery stenosis stent angioplasty. Methods: One hundred and five patients with symptomatic cerebral ischemic attack received cerebral angiography. One hundred and thirty-six narrow blood vessels were stent implanted. Results: After operation, seventy narrow blood vessels reverted to type, fifty-four maintained stenosis of less than 20%, and seven maintained stenosis of 50% to 80%. Five narrow blood vessels failed. In the operations, the thrombus protective umbrella was stemmed completely in one case. Symptoms were improved in all patients.Seventy cases were followed up by angiography between one month and twenty-four months, stenosis recurred in two cases in the carotid artery, six cases in the beginning of the vertebral artery and six cases in the intracranial artery. Conclusions: Angiography and individual therapy are helpful for cerebral artery stent angioplasty.
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    Application of the direct digital dental radiography system to diagnose proximal caries in premolars
    LIU Mei,WANG Duo,WANG Xu-xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1145)      PDF(pc) (237KB)(858)       Save
    Objective: To explore the clinical value of dental radiograph process in digital dental radiography system (DDRS)in diagnosing and treating the proximal caries. Methods: The patients, who were diagnosed as proximal caries with clinical exploration, were taken dental radiographs. The radiographs′ noises were processed and the radiographs were arranged in random order according to their computer number. One kind of image processing setup, following by another one assessed at least one week later, which could eliminate mutual obstruction, was examined jointly by three observers. Relative clinically diagnostic yields and four kinds of image processing setup were compared statistically. Results: Relative diagnostic accordance rate of the clinical exploration and four kinds of image processing setup was higher(P>0.01)in the first premolars. Relative diagnostic accordance rate of the clinical exploration and the pseudocolor image setup and negative image setup was higher(P>0.01), and that of the clinical exploration and the pseudo3D image setup and positive image setup was lower(P< 0.01), showing statistical differences in the second premolars. Conclusion: The computed image processing technique in DDRS can be applied to clinical examination of the proximal caries in premolars as an adjunct method.
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    The QRS interval for cardiac insufficiency following acute myocardial infarction
    MIAO Jun-dong,LIU Wei-hua,REN Man-yi,SUI Shu-jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1444)      PDF(pc) (275KB)(857)       Save
    To explore the role of the QRS interval for cardiac insufficiency in the immediate future following acute myocardial infarction (AMI). Methods120 patients with AMI for the first time were enrolled in the study and divided into four groups based on the heart function classification of Killip recorded within 72 hours after hospitalization. The density of brain natriuretic peptide (BNP) was determined in the plasma and the QRS interval was determined by electrocardiography. All patients were reviewed and followed up for 6 weeks and at the 6th week, the LVEF was determined by echocardiography. ResultThe QRS interval and level of BNP were increased by turns based on the heart function classified by Killip grade 1-4 and the differences were significant in each group(P<0.01). The heart function classification of Killip in the acute stage following AMI positively correlated with the BNP and QRS interval(r=0.556 and r=0.385). The log BNP and QRS interval were significantly higher in patients with heart failure than those in the control group(P<0.01). LVEF of the recovery phase negatively correlated with the BNP and QRS interval(r=-0.577 and r=-0.502). The BNP>596pg/mL and QRS interval>100ms in the acute phase were both independent prognostic factors of heart failure in the immediate future following AMI(BNP OR=13.512, P=0.009; QRS interval OR=4.696, P=0.055).
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    Expression of CD15S antigen on peripheral lymphocytes before and after acute rejection following renal transplantation
    MENG Hui-lin,LIU Yu-qiang,LV Jia-ju,LIU Jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1191)      PDF(pc) (296KB)(856)       Save
    Objective: To investigate the difference of CD15S antigen expression on peripheral lymphocytes and its clinical significance in patients before and after acute rejection and of those with a stable graft function after renal transplantation. Methods: Flow cytometry was used to detect the expression of CD15S antigen on peripheral lymphocytes in 70 patients( 95 specimens). These subjects were divided into 3 groups: patients with a stable graft function (group 1), patients with acute rejection (group 2) and patients recovered after an anti-rejection therapy (group 3). There were respectively 45, 25 and 25 subjects in each group. The percent of CD15S positive lymphocytes of each subject was detected. CD15S expression of every group was compared with a Student t test. Result: The data of group 2 were significantly higher than those of the other 2 groups, and there were no significant differences between group 1 and 3. Conclusions: CD15S expression of group 3 is equal to that of group 1. Monitoring of CD15S antigen expression on peripheral lymphocytes after renal transplantation may contribute to the diagnosis of acute rejection and may predict the effect of an anti-rejection therapy.
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