JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 39-42.doi: 10.6040/j.issn.1671-7554.0.2014.135

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Relationship between the mRNA expression of adipose triglyceride lipase in peripheral blood mononuclear cells and metabolic syndrome

JIN Chengwei1, LI Kui2, ZHAO Jing1, YUE Xin1, SHANG Yuanyuan1, HAN Lu1, ZHANG Yun1, ZHANG Wei1, MA Xiao3, ZHONG Ming1   

  1. 1. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Internal Medicine, Shandong Electric Power Central Hospital, Jinan 250001, Shandong, China;
    3. Department of Cardiology, the 456th Hospital of PLA, Jinan 250031, Shandong, China
  • Received:2014-03-13 Revised:2014-06-10 Online:2014-08-10 Published:2014-08-10

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Abstract: Objective To determine the mRNA expression of adipose triglyceride lipase (ATGL) in peripheral blood mononuclear cell (PBMC), and to explore its role in the vascular injury of metabolic syndrome (MS). Methods ATGL mRNA expression in PBMCs was determined by real time quantitative PCR in 55 controls and 56 MS patients. All subjects underwent carotid ultrasonography to measure the intima-media thickness, pressure-strain elastic modulus and stiffness. Pearson correlation was applied to assess correlation between the mRNA relative expression of ATGL and age; body mass index; blood pressure; levels of triglycerides; high-density lipoprotein cholesterol; HOMA index. Stepwise linear regression analysis was performed to examine the relationship of intima-media thickness to clinical variablesand the relative expression levels of ATGL mRNA. Results Compared with the controls, MS patients had significantly increased mRNA expression of ATGL[(7.04±3.66) vs (2.25±1.69), (P<0.001)]. Multivariate linear regression analysis showed that age, elevated triglycerides and the homeostasis model assessment index were independent risk factors for ATGL mRNA expression. Furthermore, markedly increased intima-media thickness was also found in MS patients[(0.89±0.19)mm vs (0.56±0.12)mm, (P<0.001)]. Multivariate linear regression analysis indicated the increased triglycerides and ATGL mRNA expression were independent risk factors for intima-media thickness. Conclusion The ATGL mRNA expression in peripheral blood mononuclear is associated with the progression of vascular atherosclerosis in metabolic syndrome.

Key words: Metabolic syndrome, Adipose triglyceride lipase, Carotid artery, atherosclerosis

CLC Number: 

  • R589.2
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