JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 39-42.doi: 10.6040/j.issn.1671-7554.0.2014.135

Previous Articles     Next Articles

Relationship between the mRNA expression of adipose triglyceride lipase in peripheral blood mononuclear cells and metabolic syndrome

JIN Chengwei1, LI Kui2, ZHAO Jing1, YUE Xin1, SHANG Yuanyuan1, HAN Lu1, ZHANG Yun1, ZHANG Wei1, MA Xiao3, ZHONG Ming1   

  1. 1. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Internal Medicine, Shandong Electric Power Central Hospital, Jinan 250001, Shandong, China;
    3. Department of Cardiology, the 456th Hospital of PLA, Jinan 250031, Shandong, China
  • Received:2014-03-13 Revised:2014-06-10 Online:2014-08-10 Published:2014-08-10

PDF (PC)

122

Abstract: Objective To determine the mRNA expression of adipose triglyceride lipase (ATGL) in peripheral blood mononuclear cell (PBMC), and to explore its role in the vascular injury of metabolic syndrome (MS). Methods ATGL mRNA expression in PBMCs was determined by real time quantitative PCR in 55 controls and 56 MS patients. All subjects underwent carotid ultrasonography to measure the intima-media thickness, pressure-strain elastic modulus and stiffness. Pearson correlation was applied to assess correlation between the mRNA relative expression of ATGL and age; body mass index; blood pressure; levels of triglycerides; high-density lipoprotein cholesterol; HOMA index. Stepwise linear regression analysis was performed to examine the relationship of intima-media thickness to clinical variablesand the relative expression levels of ATGL mRNA. Results Compared with the controls, MS patients had significantly increased mRNA expression of ATGL[(7.04±3.66) vs (2.25±1.69), (P<0.001)]. Multivariate linear regression analysis showed that age, elevated triglycerides and the homeostasis model assessment index were independent risk factors for ATGL mRNA expression. Furthermore, markedly increased intima-media thickness was also found in MS patients[(0.89±0.19)mm vs (0.56±0.12)mm, (P<0.001)]. Multivariate linear regression analysis indicated the increased triglycerides and ATGL mRNA expression were independent risk factors for intima-media thickness. Conclusion The ATGL mRNA expression in peripheral blood mononuclear is associated with the progression of vascular atherosclerosis in metabolic syndrome.

Key words: Metabolic syndrome, Adipose triglyceride lipase, Carotid artery, atherosclerosis

CLC Number: 

  • R589.2
[1] Zimmermann R, Strauss J G, Haemmerle G, et al. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase[J]. Science, 2004, 306(5700):1383-1386.
[2] Jenkins C M, Mancuso D J, Yan W, et al. Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities[J]. J Biol Chem, 2004, 279(47):48968-48975.
[3] Smirnova E, Goldberg E B, Makarova K S, et al. ATGL has a key role in lipid droplet/adiposome degradation in mammalian cells[J]. EMBO Rep, 2006, 7(1):106-113.
[4] Lass A, Zimmermann R, Oberer M, et al. Lipolysis-a highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores[J]. Prog Lipid Res, 2011, 50(1):14-27.
[5] Charrière G, Cousin B, Arnaud E, et al. Preadipocyte conversion to macrophage evidence of plasticity[J]. J Biol Chem, 2003, 278(11):9850-9855.
[6] 周一然, 宋建国. 脂肪组织的免疫功能[J]. 生物化学与生物物理进展, 2004, 31(8):679-683.
[7] Kassi E, Pervanidou P, Kaltsas G, et al. Metabolic syndrome: definitions and controversies[J]. BMC Med, 2011, 9(1):48.
[8] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method[J]. Methods, 2001, 25(4):402-408.
[9] 梁琳琅. 代谢综合征的诊断[J]. 中国实用内科杂志, 2008, 28(11):910-911.
[10] Koutsari C, Mundi M S, Ali A H, et al. Systemic free fatty acid disposal into very low-density lipoprotein triglycerides[J]. Diabetes, 2013, 62(7):2386-2395.
[11] Salgin B, Ong K K, Thankamony A, et al. Higher fasting plasma free fatty acid levels are associated with lower insulin secretion in children and adults and a higher incidence of type 2 diabetes[J]. J Clin Endocrinol Metab, 2012, 97(9):3302-3309.
[12] Rachek L I. Free fatty acids and skeletal muscle insulin resistance[J]. Prog Mol Biol Transl Sci, 2014, 121: 267-292. doi: 10.1016/B978-0-12-800101-1.00008-9.
[13] Boden G, Shulman G I. Free fatty acids in obesity and type 2 diabetes: defining their role in the development of insulin resistance and β-cell dysfunction[J]. Eur J Clin Invest, 2002, 32(3):14-23.
[14] Villena J A, Roy S, Sarkadi-Nagy E, et al. Desnutrin, an adipocyte gene encoding a novel patatin domain-containing protein, is induced by fasting and glucocorticoids: ectopic expression of desnutrin increases triglyceride hydrolysis[J]. J Biol Chem, 2004, 279(45):47066-47075.
[15] Festa A, D'Agostino R Jr, Howard G, et al. Chronic subclinical inflammation as part of the insulin resistance syndrome: The insulin resistance atherosclerosis study (iras)[J]. Circulation, 2000, 102(1):42-47.
[16] de Groot E, Hovingh G K, Wiegman A, et al. Measurement of arterial wall thickness as a surrogate marker for atherosclerosis[J]. Circulation, 2004, 109(23 suppl 1):33-38.
[1] HU Yanwen, WANG Zhiyuan, YU Wanjiang, ZHAO Huichen, HAN Heli, XU Zhipeng, MA Hong, ZHANG Yuchao, LIU Yuantao. Correlation of fat distribution with metabolic syndrome and glucose metabolism in 52 obese patients [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 101-106.
[2] FU Jieqi, ZHANG Man, ZHANG Xiaolu, LI Hui, CHEN Hong. Molecular mechanism of Toll-like receptor 4 in the aggravation of blood lipid accumulation by inhibiting the peroxisome proliferator-activate receptor γ [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 24-31.
[3] YIN Ni, YANG Guanlin, JIANG Junwen, WANG Chuntian, WANG Fengyao, JIA Lianqun, GAO Xiaoyu, PAN Jiaxiang, LI Qin, LI Jia, FENG Yuanjie, GAO Yuzhu, ZHOU He, ZHANG Zhe. A reliable system to assess atherosclerosis model of Bama minipigs [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 1-5.
[4] SUN Yuanying, YANG Yachao, QU Mingling, CHEN Yanmin, LI Min, WANG Shukang, XUE Fuzhong, LIU Yunxia. A prediction model for metabolic syndrome risk: a study based on the health management cohort [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(6): 87-92.
[5] YU Zhenzhen, CHEN Hui, YANG Xiaoyun, LÜ Ming. Correlation between hyperuricemi, Helicobacter pylori infection and metabolic syndrome [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 76-80.
[6] ZHANG Yunhua, LI Jie. Diagnostic value of the level of plasma lipoprotein-associated phospholipase A2 and the CEU degree of neovascularization of carotid artery plaque in acute cerebral infarction [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 112-116.
[7] WEI Dandan, ZHANG Cheng. Effects of the overexpression of type 4 angiotensin receptor on the formation of atherosclerotic lesions [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 1-5.
[8] WANG Dan, QI Hengtao, SUN Shuzhen. Changes of carotid artery structure and function in children with primary nephrotic syndrome [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(5): 88-91.
[9] SUN Pengfei, MENG Xiao, ZHANG Kai, LI Li. The effect of resistin-like molecule β on the vulnerability of atherosclerotic plaques in ApoE-/- mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 1-4.
[10] XU Yibo, JI Xiaokang, LI Xiangyi, SHEN Zhenwei, XUE Fuzhong. Analysis on the relationship between urine pH and metabolic syndrome [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(12): 82-85.
[11] XIAO Juan, CHEN Qicai, ZHANG Pengpeng, CHEN Lili, CHEN Xiaoxiao, WANG Shumei. Association between serum alanine transaminase with metabolic syndrome and its components based on a longitudinal health check-up study in Dongying City, China [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 86-91.
[12] WANG Xiaofei, WANG Chengwei, WANG Zhigang, DING Xuan, WANG Minqing. Application of enterprise stent in atherosclerotic vertebral-basilar arterial stenosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 44-48.
[13] YU Xin, LIU Xiaojing, LIU Xiangqun. The inhibitory effect of baicalin on endothelial cell apoptosis induced by ox-LDL [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 5-9.
[14] TAN Bei, GUAN Yuqing, SUN Hui, HU Keqing, SU Guohai, WEI Min. Effects of atorvastatin with intravascular ultrasound on atherosclerotic plaques in rabbits [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 10-14.
[15] DUAN Ruisheng. Immune-regulation of statins in atherosclerosis and opinions on its clinical applications [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 1-4.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd