JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (8): 1-5.doi: 10.6040/j.issn.1671-7554.0.2015.1336

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Effects of the overexpression of type 4 angiotensin receptor on the formation of atherosclerotic lesions

WEI Dandan, ZHANG Cheng   

  1. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-12-24 Online:2016-08-10 Published:2016-08-10

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Abstract: Objective To explore the effects of type 4 angiotensin receptor(AT4R)on the formation of atherosclerotic lesions and to elaborate the possible mechanism. Methods A total of 60 apolipoprotein E-deficient mice fed with high-fat diet for 4 weeks were randomly divided into 3 groups: control group(receiving infusion of PBS), Ad-EGFP group(receiving infusion of adenoviral vectors with green fluorescence protein)and Ad-AT4R group(receiving infusion of adenoviral vectors with AT4R). High-fat diet lasted for another 4 weeks and then all mice were sacrificed to collect blood samples and whole aorta. The levels of blood glucose and blood lipid were measured. The aortic plaques were stained by HE and oil red O staining method to measure the percentage of positive atherosclerotic lesion area. Results There was no significant difference in blood glucose and serum lipid levels among the 3 groups. However, compared to the control group and the Ad-EGFP group, the Ad-AT4R group showed lower positive atherosclerotic lesion area stained by HE and oil red(P<0.05). Conclusion Overexpression of AT4R may inhibit the formation of atherosclerotic lesions independent of regulation of blood glucose and serum lipid. Thus AT4R may provide protection against atherosclerosis.

Key words: Type 4 angiotensin receptor, Atherosclerosis, Formation of atherosclerotic lesions

CLC Number: 

  • R543.1
[1] Ruiz-Ortega M, Lorenzo O, Ruperez M, et al. Role of the renin-angiotensin system in vascular diseases: expanding the field[J]. Hypertension, 2001, 38(6): 1382-1387.
[2] Chai SY, Fernando R, Peck G, et al. The angiotensin Ⅳ/AT4 receptor[J]. Cell Mol Life Sci, 2004, 61(21): 2728-2737.
[3] Vanderheyden PM. From angiotensin Ⅳ binding site to AT4 receptor[J]. Mol Cell Endocrinol, 2009, 302(2): 159-166.
[4] Albiston AL, McDowall SG, Matsacos D, et al. Evidence that the angiotensin Ⅳ(AT(4))receptor is the enzyme insulin-regulated aminopeptidase[J]. J Biol Chem, 2001, 276(52): 48623-48626.
[5] Thomas WG, Mendelsohn FA. Angiotensin receptors: form and function and distribution[J]. Int J Biochem Cell Biol, 2003, 35(6): 774-779.
[6] Wright JW, Krebs LT, Stobb JW, et al. The angiotensin Ⅳ system: functional implications[J]. Front Neuroendocrinol, 1995, 16(1): 23-52.
[7] Patel JM, Martens JR, Li YD, et al. Angiotensin Ⅳ receptor-mediated activation of lung endothelial NOS is associatied with vasorelaxation[J]. 1998, 275(6 Pt 1): 1061-1068.
[8] Chen S, Patel JM, Block ER. Angiotensin Ⅳ-mediated pulmonary artery vasorelaxation is due to endothelial intracellular calcium release[J]. Am J Physiol Lung Cell Mol Physiol, 2000, 279(5): 849-856.
[9] Moeller I, Clune EF, Fennessy PA, et al. Up regulation of AT4 receptor levels in carotid arteries following balloon injury[J]. Regul Pept, 1999, 83(1): 25-30.
[10] Numaguchi Y, Ishii M, Kubota R, et al. Ablation of angiotensin Ⅳ receptor attenuates hypofibrinolysis via PAI-1 downregulation and reduces occlusive arterial thrombosis[J]. Arterioscler Thromb Vasc Biol, 2009, 29(12): 2102-2108.
[11] Yang H, Zeng XJ, Wang HX, et al. Angiotensin Ⅳ protects against angiotensin Ⅱ-induced cardiac injury via AT4 receptor[J]. Peptides, 2011, 32(10): 2108-2115.
[12] Vinh A, Widdop RE, Drummond GR, et al. Chronic angiotensin Ⅳ treatment reverses endothelial dysfunction in ApoE-deficient mice[J]. Cardiovasc Res, 2008, 77(1): 178-187.
[13] Vinh A, Widdop RE, Chai SY, et al. Angiotensin Ⅳ-evoked vasoprotection is conserved in advanced atheroma[J]. Atherosclerosis, 2008, 200(1): 37-44.
[14] 蒲丽君, 黄颂敏. 血管紧张素Ⅳ/AT4受体系统研究进展[J]. 国际泌尿系统杂志, 2006, 26(2): 283-286.
[15] Faure S, Chapot R, Tallet D, et al. Cerebroprotective effect of angiotensin Ⅳ in experimental ischemic stroke in the rat mediated by AT(4)receptors[J]. J Physiol Pharmacol, 2006, 57(3): 329-342.
[16] Hall KL, Venkateswaran S, Hanesworth JM, et al. Characterization of a functional angiotensin Ⅳ receptor on coronary microvascular endothelial cells[J]. Regul Pept, 1995, 58(3): 107-115.
[17] Davis CJ, Kramar EA, De A, et al. AT4 receptor activation increases intracellular calcium influx and induces a non-N-methyl-D-aspartate dependent form of long-term potentiation[J]. Neuroscience, 2006, 137(4): 1369-1379.
[18] Lee J, Mustafa T, McDowall SG, et al. Structure-activity study of LVV-hemorphin-7: angiotensin AT4 receptor ligand and inhibitor of insulin-regulated aminopeptidase[J]. J Pharmacol Exp Ther, 2003, 305(1): 205-211.
[19] Lew RA, Mustafa T, Ye S, et al. Angiotensin AT4 ligands are potent, competitive inhibitors of insulin regulated aminopeptidase(IRAP)[J]. J Neurochem, 2003, 86(2): 344-350.
[20] Handa RK, Harding JW, Simasko SM. Characterization and function of the bovine kidney epithelial angiotensin receptor subtype 4 using angiotensin Ⅳ and divalinal angiotensin Ⅳ as receptor ligands[J]. J Pharmacol Exp Ther, 1999, 291(3): 1242-1249.
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