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Protective effect of autophagy on the inflammation induced by ox-LDL

HAO Fengcheng1,2,  SU Zhonghua1,3   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China；
    2. Zoucheng Red Cross Emergency Center, Zoucheng 273500, Shandong, China；
    3. The Second Affiliated Hospital of Jining Medical University, Jining 272051, Shandong, China
  • Received:2013-11-20 Online:2014-04-10 Published:2014-04-10

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Abstract: Objective  To study the effect of autophagy on the inflammation induced by oxygenized low density lipoprotein (ox-LDL). Methods  The THP1 cells were divided into four groups: control group, ox-LDL treatment group, 3-methyladenine (3-MA) treatment group, ox-LDL combined with 3-MA treatment group. The expression of LC3 protein was detected by Western blotting. The formation of autophagosome was measured by immunofluorescence with LC3-specific antibody. MDC staining was used to detect the intracellular acidic vesicles. The changes of inflammatory factor level was determined by ELISA analysis. Results  Ox-LDL could induce autophagy in monocyte THP1. The inflammatory reaction induced by ox-LDL was significantly enhanced after inhibition of autophagy (P<0.05). Conclusion  Autophagy represents a defense mechanism in the inflammation induced by ox-LDL.

Key words: Atherosclerosis, Autophagy, ox-LDL, Inflammation

CLC Number: 

  • R543
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