JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (11): 13-18.doi: 10.6040/j.issn.1671-7554.0.2015.914

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Primary tumor regulates the pulmonary microenvironment in melanoma carcinoma model and facilitates lung metastasis

YUAN Bing, LI Ranran, HAN Mingyong   

  1. Department of Health Oncology, Shandong Provincal Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2015-09-25 Online:2016-11-10 Published:2016-11-10

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Abstract: Objective To explore the effect of primary melanoma on lung microenvironment and metastasis. Methods Melanoma cells(B16)were implanted into the back of Balb/c mice to build tumor-bearing mice model. Metastatic model was established by injecting B16 into the caudal vein of mice intravenously, and anti-inflammatory treatment of celecoxib was applied by gavage. Pulmonary inflammation response was analyzed by wet/dry ratios and HE staining, the circulating levels of proinflammatory cytokines in sera were evaluated by ELISA. Results In the premetastatic phase, there was a significant increase in edema and lung wet/dry ratio in experimental group compared to the control group(P<0.001). HE staining showed that a large number of inflammatory cells aggregated in the lungs of experimental group. Tumor cells tended to metastasize to where the inflammatory cells invaded. ELISA showed that compared with the control group, there was a significant increase in the levels of VEGF, M-CSF and TNF-α in the experimental group. B16 conditioned media induced inflammation cells infiltrate in lung tissues, and the number of metastatic foci in lungs lesions was higher in the experimental group than that in the control group(P<0.001). There was a significant decrease in edema, lung wet/dry ratio, and inflammation cells infiltration in the experimental group compared to the 山 东 大 学 学 报 (医 学 版)54卷11期 -袁冰,等.恶性黑色素瘤调节肺组织微环境并促进肿瘤肺转移的实验研究 \=-control group(P=0.005). Conclusion In the premetastatic phase, the melanoma induces pulmonary inflammation response, changes the lung environment and then facilitates lung metastasis. Applying of celecoxib can inhibit lung inflammation and prevent lung metastasis.

Key words: Microenvironment, Inflammation, Cytokines, Lung, Neoplasm metastasis, Melanoma

CLC Number: 

  • R73-37
[1] Huang YJ, Song N, Ding YP, et al. Pulmonary vascular destabilization in the premetastatic phase facilitates lung metastasis[J]. Cancer Res, 2009, 69(19): 7529-7537.
[2] Killock D. Cell signalling: melanoma melanosomes shape the stromal niche[J]. Nat Rev Clin Oncol, 2016, 13(10): 590-591.
[3] Carlini MJ, De Lorenzo MS, Puricelli L. Cross-talk between tumor cells and the microenvironment at the metastatic niche[J]. Curr Pharm Biotechnol, 2011, 12(11): 1900-1908.
[4] Gerber PA, Hippe A, Buhren BA, et al. Chemokines in tumor-associated angiogenesis[J]. Biol Chem, 2009, 390(12): 1213-1223.
[5] Gupta GP, Massague J. Cancer metastasis: building a framework[J]. Cell, 2006, 127(4): 679-695.
[6] Huang Y, Song N, Ding Y, et al. Pulmonary vascular destabilization in the premetastatic phase facilitates lung metastasis[J]. Cancer Res, 2009, 69(19): 7529-7537.
[7] Serafini P, Borrello I, Bronte V. Myeloid suppressor cells in cancer: recruitment, phenotype, properties, and mechanisms of immune suppression[J]. Semin Cancer Biol, 2006, 16(1): 53-65.
[8] Gassmann P, Haier J, Schlüter K, et al. CXCR4 regulates the early extravasation of metastatic tumor cells in vivo[J]. Neoplasia, 2009, 11(7): 651-661.
[9] Hiratsuka S, Watanabe A, Aburatani H, et al. Tumor mediated up-regulation of chemoattractants and recruitment of myeloid cells predetermines lung metastasis[J]. Nat Cell Biol, 2006, 8(12): 1369-1375.
[10] Krüger A. Premetastatic niche formation in the liver: emerging mechanisms and mouse models[J]. J Mol Med(Berl), 2015, 93(11): 1193-1201.
[11] Sharma SK, Chintala NK, Vadrevu SK, et al. Pulmonary alveolar macrophages contribute to the premetastatic niche by suppressing antitumor T cell responses in the lungs[J]. J Immunol, 2015, 194(11): 5529-5538.
[12] Wu CF, Andzinski L, Kasnitz N, et al. The lack of type I interferon induces neutrophil-mediated pre-metastatic niche formation in the mouse lung[J]. Int J Cancer, 2015, 137(4): 837-847.
[13] Yan HH, Jiang J, Pang Y, et al. CCL9 induced by TGF-β signaling in myeloid cells enhances tumor cell survival in the premetastatic organ[J]. Cancer Res, 2015, 75(24): 5283-5298.
[14] Souza LE, Almeida DC, Yaochite JN, et al. Intravenous administration of bone marrow-derived multipotent mesenchymal stromal cells enhances the recruitment of CD11b+ myeloid cells to the lungs and facilitates B16-F10 melanoma colonization[J]. Exp Cell Res, 2015, 345(2): 141-149.
[15] Kaplan RN, Rafii S, Lyden D. Preparing the “soil”: the premetastatic niche[J]. Cancer Res, 2006, 66(23): 11089-11093.
[16] Mantovani A, Allavena P, Sica A, et al. Cancer-related inflammation[J]. Nature, 2008, 454(7203): 436-444.
[17] Kulbe H, Chakravarty P, Leinster DA, et al. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment[J]. Cancer Res, 2012, 72(1): 66-75.
[18] DeNardo DG, Coussens LM. Inflammation and breast cancer. Balancing immune response: crosstalk between adaptive and innate immune cells during breast cancer progression[J]. Breast Cancer Res, 2007, 9(4): 212.
[19] Dawson MR, Duda DG, Fukumura D, et al. VEGFR1 activity independent metastasis formation[J]. Nature, 2009, 461(7262): E4-E5.
[20] Solinas G, Marchesi F, Garlanda C, et al. Inflammation-mediated promotion of invasion and metastasis[J]. Cancer Metastasis Rev, 2010, 29(2): 243-248.
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