JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (3): 83-87.doi: 10.6040/j.issn.1671-7554.0.2016.781

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Expression and significance of CK14 in non-small cell lung cancer

WANG Yongqiang1, ZHANG Weiquan1, SUN Qifeng1, DONG Xiaopeng1, PENG Chuanliang1, ZHANG Yuan2, ZHAO Xiaogang1   

  1. 1. Department of Thoracic Surgery;
    2. Evidence Based Medicine Center, the Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2016-07-05 Online:2017-03-10 Published:2017-03-10

Abstract: Objective To investigate the expression of CK14 and its significance in the occurrence, development and metastasis of non-small cell lung cancer(NSCLC). Methods A total of 110 NSCLC patients were involved in the study. The NSCLC cancerous tissues, the tissues adjacent to carcinoma and normal lung tissues of 110 patients and 12 patients were analyzed for CK14 expression by immunohistochemistry and Western blotting, respectively. The relationship between CK14 expression and clinical pathological parameters of NSCLC patients was analyzed by non-conditional Logistic regression analysis. Results The immunohistochemistry results showed that CK14 was overexpressed in NSCLC cancerous tissues which was significantly higher than tissues adjacent to carcinoma and normal lung tissues(P<0.001). In addition, the positive rate of CK14 was 68.18%. Its level was significantly correlated with sex, histopathologic type, differentiation of tumors, clinical stages and lymph node metastasis(P<0.05). However, no correlation was observed between CK14 level and age(P>0.05). Additionally, Western blotting result also showed that CK14 was overexpressed in NSCLC cancerous tissues compared with the tissues adjacent to carcinoma and normal lung tissues. The results of non-conditional Logistic regression analysis showed that poor differentation, staging Ⅱ and Ⅲ, and the positive expression of CK14 were important risk factors in lymph node metastasis of NSCLC(P<0.05). Conclusion CK14 plays a promotional role in the development and metastatic progression of NSCLC. CK14 expression would be a significant biomarker in evaluating the biological malignant behavior of NSCLC.

Key words: Carcinoma, non-small-cell lung, Cytokeratin 14, Logistic regression analysis, Immunohistochemistry

CLC Number: 

  • R734.2
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