JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 81-85.doi: 10.6040/j.issn.1671-7554.0.2014.465

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Expression of T cell immunoglobulin mucin-3 in villous and decidual tissues of patients with unexplained recurrent spontaneous abortion

JIA Qiuhua1,2, YU Fei3   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Maternal and Children Health Hospital of Linyi City, Linyi 276000, Shandong, China;
    3. Affiliated Hospital of Shandong Medical College, Linyi 276000, Shandong, China
  • Received:2014-07-15 Revised:2014-09-18 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To explore the expression of T cell immunoglobulin mucin-3 (Tim-3) in villous and decidual tissues of patients with unexplained recurrent spontaneous abortion (URSA). Methods A total of 30 pregnant women with URSA treated in Maternal and Children Health Hospital of Linyi City during March 2012 to June 2014 were involved as the case group, and 30 normal pregnant women who had undergone selective abortion (gestational age 6 to 12 weeks) in the same period were chosen as the control group. The location and protein level of Tim-3 in villous and decidual tissues of the two groups were measured with immunohistochemistry technique. Results Immunohistochemical staining results showed that in the two groups, Tim-3 protein was mainly located in the cytoplasm of villi trophoblast cells and form-fitting trophoblastic cells, villi interstitial, cytoplasm of glandular epithelial cells of the decidual tissue, and decidual stromal. The expression of Tim-3 in the villous tissues and glands of decidual tissues of the case group was stronger than that of the control group (P<0.001). In the villous tissues of the case group, 2 cases had weak expression of Tim-3, 5 had positive expression, and 23 had strong positive expression. In the villous tissues of the control group, 22 cases had weak expression of Tim-3, 6 had positive expression, and 2 had strong positive expression. In the decidualtissues of the case group, 4 cases had weak expression of Tim-3, 5 had positive expression, and 21 had strong positive expression. In the decidual tissues of the control group, 26 cases had weak expression of Tim-3, 2 had positive expression, and 2 had strong positive expression. Conclusion Tim-3 might take part in the genesis and development of URSA.

Key words: Unexplained recurrent spontaneous abortion, Villous tissues, Immunohistochemistry, Tim-3, Decidual tissues

CLC Number: 

  • R714.2
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