JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 72-76.doi: 10.6040/j.issn.1671-7554.0.2014.314

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Expression of autophagy pathway proteins eIF4E and 4EBP1 in several cerebroma tissues

XU Jiajun1,2, LIU Zongxian1, XU Fenglei3, WANG Lei1, KONG Weihua1   

  1. 1. School of Life Science, Shandong University, Jinan 250100, Shandong, China;
    2. Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    3. Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-05-15 Revised:2014-09-06 Online:2014-09-10 Published:2014-09-10
  • Contact: 孔维华。E-mail:whkong@sdu.edu.cn E-mail:whkong@sdu.edu.cn

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Abstract: Objective To explore the expressions of 4EBP1 and eIF4E in astrocytoma, meningeoma, oligdendrocytoma, cholesteatoma and normal brain tissues and their differences. Methods Reverse transcriptase polymerase chain reaction (RT-PCR) was adopted to determine the levels of 4EBP1 and eIF4E mRNAs in four cerebromas. Both immunohistochemistry and Western blotting methods were applied to detect the expressions of eIF4E and 4EBP1 proteins in four cerebromas. SPSS 17.0 was used to analyze the differences of expressions of 4EBP1 and eIF4E in four cerebromas as well as normal tissues. Results The mRNAs and proteins of eIF4E and 4EBP1 were observed in four cerebromas. Meanwhile, the proteins of LC-3 and LAMP-2A increased significantly. The results of immunohistochemistry suggested that the levels of eIF4E and 4EBP1 were higher in meningeoma, cholesteatoma, astrocytoma and oligdendrocytoma compared with those in normal tissues. The results of analysis of variance showed that in comparison to normal brain tissues, the expressions of eIF4E and 4EBP1 in astrocytoma, meningeoma, oligdendrocytoma, and cholesteatoma hadstatistically significant (P<0.01). Conclusion The expressions of eIF4E and 4EBP1 are obviously up-regulated in four cerebromas, which suggests that eIF4E, 4EBP1 and their autophagy signaling pathways play an important role in the development of cancer; therefore, eIF4E and 4EBP1 can act as potential therapeutic targets as well as evidence for clinical diagnosis.

Key words: Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E-binding protein 1, Cerebroma, Immunohistochemistry

CLC Number: 

  • R739.4
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