JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (5): 62-65.doi: 10.6040/j.issn.1671-7554.0.2015.839

Previous Articles     Next Articles

Effects of prednisone therapy on the clinical outcomes and phenotypes of natural killer cells in patients with URSA

JIA Xinzhuan1, ZHANG Na1, GUO Lina1, ZHAO Suying1, DONG Cui1, WEI Lan2   

  1. 1. Department of Reproductive Medicine, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China;
    2. Department of Thoracic Surgery, Hebei Provincial Chest Hospital, Shijiazhuang 050041, Hebei, China
  • Received:2015-09-05 Online:2016-05-16 Published:2016-05-16

PDF (PC)

299

Abstract: Objective To analyze the clinical outcomes(ongoing pregnancy > 12 weeks)and changes of natural killer(NK)cells CD56 and CD16 before and after prednisone treatment in patients with URSA(unexplained recurrent spontaneous abortion, URSA). Methods A total of 160 URSA patients were divided into two groups: experimental group(receiving prednisone and aspirin therapy, n=80), and control group(receiving placebo and aspirin therapy, n=80). The therapy started from 4-5 weeks to 12 weeks of gestation, and stopped if abortion occurred. The percentages of CD56 and CD16 were measured by flow cytometry before and after treatment. Results The percentage of ongoing pregnancy > 12 weeks was significantly higher in the experimental group than in the control group(P=0.00). The level of CD16 markedly dropped after treatment in the experimental group(P=0.00), while it remained unchanged in the control group(P=0.79). There was no statistical difference in the level of CD56 before and after treatment between the two groups(experimental group P=0.09, control group P=0.12). Conclusion Prednisone can improve the clinical outcome in patients with URSA, which may be related to the reduction of CD16 and inhibition of cell toxicity.

Key words: Unexplained recurrent spontaneous abortion, Prednisone, CD56, CD16, Natural Killer cell

CLC Number: 

  • R714.2
[1] Practice Committee of American Society for Reproductive Medicine. Definitions of infertility and recurrent pregnancy loss: a committee opinion[J]. Fertil Steril, 2013, 99(1): 63. doi:10:1016/j.fertnstert.2012.09.023. Epub 2012 Oct 22.
[2] Beaman KD, Ntrivalas E, Mailers TM, et al. Immune etiology of recurrent pregnancy loss and its diagnosis[J]. Am J Reprod Immunol, 2012, 67(4): 319-325.
[3] Kwak-Kim J, Park JC, Ahn HK, et al. Immunological modes of pregnancy loss[J]. Am J Reprod Immunol, 2010, 63: 611-623. doi: 10.1111/j.1600-0897.2010.00847.x. Epub 2010 Mar 29.
[4] Bansal AS. Joining the immunological dots in recurrent miscarriage[J]. Am J Reprod Immunol, 2010, 64(5): 307-315.
[5] Lee RM, Silver RM. Recurrent pregnancy loss: summary and clinical recommendations[J]. Semin Reprod Med, 2000,18(4): 433-440.
[6] Ksouri H, Zitouni M, Achour W, et al. Recurrent pregnancy loss related to immune disorders[J]. Ann Med Intern, 2003, l54(4): 233-247.
[7] Lash GE, Robson SC, Bulmer JN. Review: Functional role of uterine natural killer(uNK)cells in human early pregnancy decidua[J]. Placenta, 2010, 31(Suppl.A): S87-92. doi: 10.1016/j.placenta.2009.12.022. Epub 2010 Jan 12.
[8] 贾秋桦, 于斐. Tim-3在不明原因复发性流产患者绒毛和蜕膜组织中的表达[J]. 山东大学学报(医学版), 2014, 52(10): 81-85. JIA Qiuhua, YU Fei. Expression of T cell immunoglobulin mucin-3 in villous and decidual tissues of patients with unexplained recurrent spontaneous abortion[J]. Journal of Shandong University(Health Sciences), 2014, 52(10): 81-85.
[9] Kusnierczyk P. Killer cell immunoglobulin-like receptor gene associations with autoimmune and allergic diseases, recurrent spontaneous abortion, and neoplasms[J]. Front Immunol, 2013, 29(4): 8.
[10] Sacks G, Yang Y, Gowen E, et al. Detailed analysis of peripheral blood natural killer cells in women with repeated IVF failure[J]. Am J Reprod Immunol, 2012, 67(5): 434-442.
[11] Avril T, Iochmann S, Brand D, et al. Human choriocarcinoma cell resistance to natural killer lysis due to defective triggering of natural killer cells[J]. Biol Reprod, 2003, 69(2): 627-633.
[12] Paparistidis N, Papadopoulou C, Chioti A, et al. How valuable is measurement of peripheral blood natural killer cells at the time of abortion?[J]. Am J Reprod Immunol, 2008, 59(4): 306-315.
[13] Michou VI, Kanavaros P, Athanassiou V, et al. Fraction of the peripheral blood concentration of CD56+/CD16-/CD3-cells in total natural killer cells as an indication of fertility and infertility[J]. Fertil and Steril, 2003, 80(Suppl 2): 691-697. doi:10.1016/S0015-0282(03)00778-7.
[14] Gomaa MF, Elkholy AG, El-Said MM, et al. Combined oral prednisolone and heparin versus heparin: the effect on peripheral NK cells and clinical outcome in patients with unexplained recurrent miscarriage. A double-blind placebo randomized controlled trial[J]. Arch Gynecol Obstet, 2014, 290(4): 757-762.
[1] DONG Xiaojing, LI Jing, ZHAO Lei, QI Mei, SUN Zeyu, WANG Hong, ZHAO Weiming. Effect of natural killer cells on the production of interleukin-22 and disease process during Chlamydia muridarum lung infection [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(11): 19-23.
[2] JIA Qiuhua, YU Fei. Expression of T cell immunoglobulin mucin-3 in villous and decidual tissues of patients with unexplained recurrent spontaneous abortion [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(10): 81-85.
[3] LI Ling, CHEN Yong, YANG Chong-mei. Correlation study of single nucleotide polymorphism of multiple
drug resistance 1 and ulcerative colitis prednisone sensitivity [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 99-102.
[4] BIAN Xiao-kun1， SI Yuan-quan1， LU Nan3， LI Ying-Jie1， JIA Yan-fei2， ZHANG Yi1. Expression and clinical significance of immunoglobulin-like transcript 2 in gastric cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 117-.
[5] YIN Xian-ming1, DONG Bai-hua1, ZHANG Yun2, SU Shi-li1, DONG Zhao-gang2, QU Xun2. Expression of integrin αvβ3 on NK cells of women with  early pregnancy or missed abortion [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 140-.
[6] WANG He-li1, SUN Xue-mei2, SUN Zheng-yun1, WANG Na1, LIN Xia1. Changes of CD3+, CD4+ and CD8+ T cells and natural killer cells in neonates born to gestational diabetes mellitus mothers and  their relationship with blood glucose [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 4-.
[7] . Effects of trichostatin A effects on natural killer cell  survival and activity [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(8): 50-54.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd