JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (11): 19-23.doi: 10.6040/j.issn.1671-7554.0.2015.1067

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Effect of natural killer cells on the production of interleukin-22 and disease process during Chlamydia muridarum lung infection

DONG Xiaojing1, LI Jing1, ZHAO Lei2, QI Mei1, SUN Zeyu1, WANG Hong1, ZHAO Weiming1   

  1. 1. Department of Medical Microbiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China; 2. Institute of Basic Medical Science, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-04 Online:2016-11-10 Published:2016-11-10

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Abstract: Objective To evaluate the effect of natural killer(NK)cells on the production of interleukin-22(IL-22)and disease process in Chlamydia lung infection. Methods Thirty-six mice were divided into experimental group(n=18)and control group(n=18). Mice in experimental group or control group were injected anti-asialo GM1 antibody or isotype respectively, and then infected with Chlamydia muridarum intranasally. Body weight changes were monitored daily. Mice were sacrificed at day 6 and day 12 after infection, and inclusion-forming units(IFU)of lungs were measured. IL-22 expression was determined by real-time PCR and ELISA. The changes of Th17 cells numbers were measured by flow cytometry analysis. Results Comparied with control group, mice in experimental group deepened the lung infection and body weight decrease trend, exhibited the incresed lung IFUs, reduced splenic IL-22 expressions in mRNA and protein levels(all P<0.05), and decreased percentage and absolute numbers of Th17 cells in spleen(both P<0.01). Conclusion NK cells depletion results in reduced IL-22 expression and severe lung disease. NK cells can promote the expression of IL-22, which contributes to the effective control of Chlamydial lung infection.

Key words: Chlamydia, Natural killer cells, Interleukin-22

CLC Number: 

  • R374.1
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