JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 1-6.doi: 10.6040/j.issn.1671-7554.0.2015.001

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IL-22 protects mice from acute severe pancreatitis via STAT3 signaling pathway

BAI Jinxia1, BAI Jinyun2, SHI Xiuju1, XU Hongwei1   

  1. 1. Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2015-01-04 Revised:2015-03-31 Online:2015-06-10 Published:2015-06-10

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Abstract: Objective To investigate the effect of interleukin-22 (IL-22) in acute severe pancreatitis (SAP) induced by L-arginine and its signal pathway. Methods Sixty male Balb/c mice were randomly divided into 4 groups, the normal control group(NaCl group, n=10), SAP group(n=30), treatment control group (PBS group, n=10) and treatment group(rIL-22 group, n=10). Mice were induced intraperitoneally with L-arginine(two doses, 4 g/kg each, 1 hour apart). PBS or rIL-22 (200 ng/time, 5 times) was administrated to mice at the indicated times in PBS and rIL-22 groups. Serum amylase and pancreas tissue histopathology were examined. IL-22RA1, regenerating islet-dedrived protein 3β(Reg3β), Reg3γ, B cell lymphoma/lewmia-2(Bcl-2), Bcl-xL mRNA were detected by Real-time PCR. STAT3 expression and activation were assessed by Western blotting. Mortality ratios in PBS and IL-22 group were counted. Results Seventy-two hours after L-arginine administration, typically histopathological changes were observed in SAP group. The expressions of Reg3β, Reg3γ, Bcl-2 and Bcl-xL mRNA decreased progressively, while serum amylaseand IL-22RA1 mRNA increased until 48 hours, but decreased apparently at 72 hours. Compared with those in PBS group, rIL-22 group showed lower serum amylase(P<0.05), slighter pathological changes and lower mortality(P<0.05). Compared with those in PBS group, the expressions of Reg3β, Reg3γ, Bcl-2, Bcl-xL, IL-22RA1 mRNA and phospho-STAT3 protein in rIL-22 group markedly increased(P<0.05). Conclusion Exogenous recombinant IL-22 protects mice from L-arginine induced SAP by enhancing the expressions of antimicrobial peptides and antiapoptotic genes through the STAT3 signaling pathway.

Key words: Signal transducer and activator transcription 3, Interleukin-22, Acute severe pancreatitis, Antimicrobial peptide

CLC Number: 

  • R576
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