JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 36-40.doi: 10.6040/j.issn.1671-7554.0.2014.929

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Effects of CD40 knockdown by siRNA on rats with experimental autoimmune myocarditis and IL-22 expression

ZHANG Rongjun1, HAN Bo1, GAO Ling2, ZHU Mei3, DING Guoyu1, LIANG Yan1   

  1. 1. Department of Pediatric Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    3. Department of Ultrasonography, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2014-12-09 Revised:2015-03-18 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To explore the effects of CD40 knockdown by siRNA on myocardial tissues, cTNT, BNP and IL-22 in rats with experimental autoimmune myocarditis (EAM). Methods A total of 48 male Lewis rats aged 6-8 weeks were randomly divided into normal control group, EAM group, CD40 siRNA group and siRNA group, with 12 rats in each group. On day 21, 6 rats were sacrificed respectively in each group; on day 56, the remaining rats were killed. The histopathologic and ultrastructure changes were observed with light and electron microscope. The myocardial histopathologic scores were counted, and cTNT, BNP and IL-22 were tested with ELISA. Results On day 21 and 56, the total incidence rate of each group was 100% except the normal control group, and there was no statistical difference in survival rate and mortality rate in each group (P>0.05). Myocardial histopathological scores, serum levels of cTNT and BNP in CD40 siRNA group were significantly lower than those in EAM group (P<0.05), while the serum level of IL-22 was higher than that in EAM group (P<0.05), and the serum levels of cTNT and BNP were positively correlated with myocardial histopathological scores (r=0.732, r=0.869, P<0.05). Conclusion Silencing CD40 by siRNA can relieve inflammation in EAM, alleviate myocardial injury and improve heart function. The mechanism may involve the up-regulation of IL-22 expression and inhibition of immune response.

Key words: Rats, Brain natriuretic peptide, CD40 siRNA, Autoimmune myocarditis, Cardiac troponin, Interleukin-22

CLC Number: 

  • R542.2
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