JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (11): 13-18.doi: 10.6040/j.issn.1671-7554.0.2015.914

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Primary tumor regulates the pulmonary microenvironment in melanoma carcinoma model and facilitates lung metastasis

YUAN Bing, LI Ranran, HAN Mingyong   

  1. Department of Health Oncology, Shandong Provincal Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2015-09-25 Online:2016-11-10 Published:2016-11-10

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Abstract: Objective To explore the effect of primary melanoma on lung microenvironment and metastasis. Methods Melanoma cells(B16)were implanted into the back of Balb/c mice to build tumor-bearing mice model. Metastatic model was established by injecting B16 into the caudal vein of mice intravenously, and anti-inflammatory treatment of celecoxib was applied by gavage. Pulmonary inflammation response was analyzed by wet/dry ratios and HE staining, the circulating levels of proinflammatory cytokines in sera were evaluated by ELISA. Results In the premetastatic phase, there was a significant increase in edema and lung wet/dry ratio in experimental group compared to the control group(P<0.001). HE staining showed that a large number of inflammatory cells aggregated in the lungs of experimental group. Tumor cells tended to metastasize to where the inflammatory cells invaded. ELISA showed that compared with the control group, there was a significant increase in the levels of VEGF, M-CSF and TNF-α in the experimental group. B16 conditioned media induced inflammation cells infiltrate in lung tissues, and the number of metastatic foci in lungs lesions was higher in the experimental group than that in the control group(P<0.001). There was a significant decrease in edema, lung wet/dry ratio, and inflammation cells infiltration in the experimental group compared to the 山 东 大 学 学 报 (医 学 版)54卷11期 -袁冰,等.恶性黑色素瘤调节肺组织微环境并促进肿瘤肺转移的实验研究 \=-control group(P=0.005). Conclusion In the premetastatic phase, the melanoma induces pulmonary inflammation response, changes the lung environment and then facilitates lung metastasis. Applying of celecoxib can inhibit lung inflammation and prevent lung metastasis.

Key words: Microenvironment, Inflammation, Cytokines, Lung, Neoplasm metastasis, Melanoma

CLC Number: 

  • R73-37
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