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    10 November 2016
    Volume 54 Issue 11
    Current situation and development trend of CAR-T in anti-tumor research
    ZHENG Min, ZHANG Lan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  1-6.  doi:10.6040/j.issn.1671-7554.0.2016.835
    Abstract ( 693 )   PDF (1250KB) ( 326 )   Save
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    A lot of remarkable results suggested that the modification of T-cells with CARs(CRA-T)could be a powerful approach for developing safe and effective cancer therapeutics. A novel breakthrough has been made in CAR-T treatment of hematologic tumors and therapeutic potential in clinical studies of solid tumors has been achieved, although there are still many problems for CAR-T on the clinical application such as off-target effects, cytokine storm, insertion mutagenesis, limited effects for solid tumors. Here, we reviewed the recent advances of CAR-T and problems that need to be solved in this field, which provide references for the in-depth study and further clinical immunotherapy.
    Mechanical function of HIPK2 in epithelial-mesenchymal transition and metastasis of non-small cell lung cancer
    ZHENG Hui, WEI Guangwei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  7-12.  doi:10.6040/j.issn.1671-7554.0.2016.227
    Abstract ( 819 )   PDF (3801KB) ( 303 )   Save
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    Objective To investigate the role of homeodomain interacting protein kinase 2(HIPK2)in epithelial-mesenchymal transition(EMT)and metastasis in non-small cell lung cancer(NSCLC)cells, and to explore the possible mechanism. Methods The A549 and H520 cells were divided into high expression group and control group, then transfected with pcDNA3.1-HIPK2 and pcDNA3.1-Vector with Lipofectamine 2000. HIPK2 silenced and control cell lines were constructed in A549 by the transfection of GV248 shHIPK2 and GV248 shVector with lentivirus. GV248 shZEB1 and GV248 shVector were transfected with Lipofectamine 2000 in A549 cells in which HIPK2 was silenced. The expression of HIPK2 and EMT markers were detected by Western blotting. The migration and invasion ability of cells were detected through wound scratch assay, Transwell assay and Matrigel assay. Results Compared with control group, the expression of epithelial cell markers in high expression group were enhanced(P<0.01), and the expression of mesenchymal cell markers were descended(P<0.01), which repressed the EMT process. Meanwhile, wound scratch assay showed that the migrated distance of high expression group were less than the control group(P<0.05). Transwell assay and Matrigel assay showed that the number of cells that migrated and invaded through the membrane were obviously 山 东 大 学 学 报 (医 学 版)54卷11期 -郑荟,等.HIPK2抑制非小细胞肺癌上皮间质转化及迁移侵袭的作用及机制 \=-less than those in control group(P<0.01). Conversely, silencing HIPK2 generated the opposite effects. The enhanced EMT and metastasis induced by HIPK2 silencing could be reversed by interfering zinc finger E-box binding homeobox 1(ZEB1). Conclusion HIPK2 represses EMT, migration and invasion of NSCLC cells, and the possible mechanism is related to transcription factor ZEB1.
    Primary tumor regulates the pulmonary microenvironment in melanoma carcinoma model and facilitates lung metastasis
    YUAN Bing, LI Ranran, HAN Mingyong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  13-18.  doi:10.6040/j.issn.1671-7554.0.2015.914
    Abstract ( 866 )   PDF (7565KB) ( 274 )   Save
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    Objective To explore the effect of primary melanoma on lung microenvironment and metastasis. Methods Melanoma cells(B16)were implanted into the back of Balb/c mice to build tumor-bearing mice model. Metastatic model was established by injecting B16 into the caudal vein of mice intravenously, and anti-inflammatory treatment of celecoxib was applied by gavage. Pulmonary inflammation response was analyzed by wet/dry ratios and HE staining, the circulating levels of proinflammatory cytokines in sera were evaluated by ELISA. Results In the premetastatic phase, there was a significant increase in edema and lung wet/dry ratio in experimental group compared to the control group(P<0.001). HE staining showed that a large number of inflammatory cells aggregated in the lungs of experimental group. Tumor cells tended to metastasize to where the inflammatory cells invaded. ELISA showed that compared with the control group, there was a significant increase in the levels of VEGF, M-CSF and TNF-α in the experimental group. B16 conditioned media induced inflammation cells infiltrate in lung tissues, and the number of metastatic foci in lungs lesions was higher in the experimental group than that in the control group(P<0.001). There was a significant decrease in edema, lung wet/dry ratio, and inflammation cells infiltration in the experimental group compared to the 山 东 大 学 学 报 (医 学 版)54卷11期 -袁冰,等.恶性黑色素瘤调节肺组织微环境并促进肿瘤肺转移的实验研究 \=-control group(P=0.005). Conclusion In the premetastatic phase, the melanoma induces pulmonary inflammation response, changes the lung environment and then facilitates lung metastasis. Applying of celecoxib can inhibit lung inflammation and prevent lung metastasis.
    Effect of natural killer cells on the production of interleukin-22 and disease process during Chlamydia muridarum lung infection
    DONG Xiaojing, LI Jing, ZHAO Lei, QI Mei, SUN Zeyu, WANG Hong, ZHAO Weiming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  19-23.  doi:10.6040/j.issn.1671-7554.0.2015.1067
    Abstract ( 880 )   PDF (1866KB) ( 210 )   Save
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    Objective To evaluate the effect of natural killer(NK)cells on the production of interleukin-22(IL-22)and disease process in Chlamydia lung infection. Methods Thirty-six mice were divided into experimental group(n=18)and control group(n=18). Mice in experimental group or control group were injected anti-asialo GM1 antibody or isotype respectively, and then infected with Chlamydia muridarum intranasally. Body weight changes were monitored daily. Mice were sacrificed at day 6 and day 12 after infection, and inclusion-forming units(IFU)of lungs were measured. IL-22 expression was determined by real-time PCR and ELISA. The changes of Th17 cells numbers were measured by flow cytometry analysis. Results Comparied with control group, mice in experimental group deepened the lung infection and body weight decrease trend, exhibited the incresed lung IFUs, reduced splenic IL-22 expressions in mRNA and protein levels(all P<0.05), and decreased percentage and absolute numbers of Th17 cells in spleen(both P<0.01). Conclusion NK cells depletion results in reduced IL-22 expression and severe lung disease. NK cells can promote the expression of IL-22, which contributes to the effective control of Chlamydial lung infection.
    Effects of curcumin on gene expressions of reverse cholesterol transport proteins ABCA1versus ABCG1 in the cell model of inflammation
    WU Yu, XIA Yanqing, WU Yue, SUN Lin, WU Chengye, LOU Guiyu, LI Yaxin, DING Songze, SUN Kai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  24-26.  doi:10.6040/j.issn.1671-7554.0.2015.542
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    Objective To explore the effects of curcumin on reverse cholesterol transport protein gene expression in the cell model of inflammation. Methods RAW264.7 cells were divided into 6 groups: control group, 10 μmol/L curcumin group, 20 μmol/L curcumin group, lipopolysaccharide(LPS)group, 10 μmol/L curcumin+LPS group, and 20 μmol/L curcumin+LPS group. The levels of mRNA expressions of ATP-binding cassette transporter A1(ABCA1), ATP-binding cassette transporter G1(ABCG1)and tumor necrosis factor-α(TNF-α)in each group were determined by Real-Time PCR. Results LPS induction significantly increased ABCA1 and TNF-α mRNA expressions, and decreased ABCG1 mRNA expression(all P<0.05). Pre-treating the cell with curcumin and stimulating with LPS did not change the expressions of TNF-α and ABCG1(both P>0.05), but significantly increased the ABCA1 mRNA expression(P<0.05). Conclusion Curcumin significantly amplifies the expression of reverse cholesterol transport key genes ABCA1 under the condition of the inflammatory reaction, which may be one of the molecular mechanisms of anti-arteriosclerosis.
    Inhibitory effect of miR-98-5p on human gastric cancer MGC803 cell proliferation by regulating AKT2 expression
    LI Shuai, LI Dinuo, YUAN Chaofan, LIANG Xu, WANG Yubin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  27-31.  doi:10.6040/j.issn.1671-7554.0.2015.1266
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    Objective To investigate the expressions of miR-98-5p and AKT2 in human gastric cancer MGC803 cells, in order to explore the regulatory effect of miR-98-5p on AKT2 and on the proliferation of MGC803 cells. Methods MGC803 cells were cultured in vitro and cellular morphology changes were observed with phase contrast microscope. The expression of AKT2 in MGC803 cells was determined with immunocytochemistry. The regulatory relation of miR-98-5p and AKT2 was predicted by bioinformatics and identified using dual luciferase report system. Expressions of miR-98-5p and AKT2 were determined using real-time PCR and Western blotting after transfection of miR-98-5p mimics. The proliferation of MGC803 cells was detected by MTT assay and plate colony formation assay. Results Under phase contrast microscope, most of the MGC803 cells were fusiform or polygonal, few were quasi-circular. Immunocytochemistry indicated AKT2 expression in the cytoplasm, which looked sepia. MiRanda and TargetScan showed that miR-98-5p was well matched with AKT2 gene. Dual luciferase reporter system indicated that miR-98-5p could inhibit the expression of AKT2 by binding to its mRNA 3'UTR. Real-time PCR and Western blotting showed that over-expression of miR-98-5p down-regulated expression of AKT2 and suppressed the proliferation of MGC803 cells. Conclusion The miR-98-5p may negatively regulate the expression of AKT2 in human gastric cancer MGC803 cells and inhibit cell proliferation.
    Value of thoracoscope combined with urokinase treatment on loculated pleural effusion
    YAN Di, MA Weixia, JIANG Shujuan, SHI Xinyu, WANG Na
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  32-35.  doi:10.6040/j.issn.1671-7554.0.2015.1217
    Abstract ( 998 )   PDF (892KB) ( 279 )   Save
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    Objective To evaluate the value of medical thoracoscope combined with urokinase treatment on loculated pleural effusion. Methods Fifty-eight patients with loculated pleural effusion were recruited and divided into the treatment group(n=27)and the control group(n=31). The treatment group was treated with medical thoracoscope and urokinase(1×105 U, twice a week)while the control group was given routine drainage. The total drainage volume, pleural thickness, protein content of pleural effusion, duration of pleural effusion, incidence of complication and therapeutic effect were compared. Results The total drainage volume in the treatment group was higher than that in the control group[(1141.51±411.66)vs(751.93±605.53)mL, P<0.05]; duration of the pleural effusion, pleural thickness and protein content of pleural effusion in the treatment group were lower than those in the control group[(6.18 ±1.88)vs(7.54±2.28)days,(2.09±0.50)vs(2.90±0.57)mm,(26.45 ±12.09)vs(34.33±10.99)g, all P<0.05]; medical effective rate in treatment group was higher than that in the control group(96.3% vs 77.4%, P<0.001). The incidence of complications in the two groups had no significant difference(P>0.05). Conclusion The value of the medical thoracoscopy combined with urokinase treatment on loculated pleural effusion is significant. The pleural effusion is drained thoroughly and rapidly. This method can increase the duration of the pleural effusion, relieve pleural thickening, and cause no obvious increase in complications.
    Diagnostic value of combination of PCT and NSE in serum for detecting small cell lung cancer
    ZHANG Zhiqiang, WU Yanhua, WANG Maoshui, WANG Xinfeng, WANG Yunshan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  36-39.  doi:10.6040/j.issn.1671-7554.0.2015.412
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    Objective To investigate the diagnostic value of combination of procalcitonin(PCT)and neuron-specific enolase(NSE)in serum for differentiating small cell lung cancer from other lung cancers. Methods A total of 123 lung cancer patients without lung infections, other sites infection history and liver metastasis were divided into three groups: squamous carcinoma group(n=48), adenocarcinoma group(n=52)and small cell carcinoma group(n=23). And 40 healthy volunteers were taken as control group. Serum PCT and NSE levels were tested and compared among each group. Results Serum PCT and NSE levels were statistical different among the four groups(P<0.01). Mann-Whitney analysis showed that, there were statistical differences between small cell carcinoma and squamous carcinoma or adenocarcinoma groups(P<0.05, P<0.01). For PCT and NSE, the area under the ROC curve was 0.671 and 0.806, the best cut-off point was 0.10 and 23.91 ng/mL, sensitivity was 56.5% and 69.6%, and specificity was 77.0% and 90.0%. Combination(PCT+NSE)would increase the specificity to 98.0%, while combination(PCT or 山 东 大 学 学 报 (医 学 版)54卷11期 -张志强,等.联合检测血清PCT和NSE对小细胞肺癌的诊断价值 \=-NSE)would increase the sensitivity to 82.6%. Conclusion PCT can be used as a marker in differentiating small cell lung cancer from other lung cancers. However, for the small cell lung cancer patients, serum PCT cannot be simply used to detect the infection. Meanwhile, combined biomarkers(PCT and NSE)will improve the diagnostic performance in discrimination of small cell lung cancer.
    Retrospective analysis of 36 critical patients treated by extracorporeal menlbrarle oxygenation
    CHEN Chunyan, LIU Xiaojun, QI Shaoyan, WANG Wentao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  40-43.  doi:10.6040/j.issn.1671-7554.0.2015.835
    Abstract ( 839 )   PDF (892KB) ( 203 )   Save
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    Objective To investigate the therapeutic effects and safety of extracorporeal menlbrarle oxygenation(ECMO)on critical patients with respiratory or circulatory failure. Methods Medical records of 36 critical patients treated by ECMO from August 2012 to April 2015 in the Second Affiliated Hospital of Zhengzhou University were reviewed; the prognosis, complications and influencing factors were summarized and analyzed. Results Survival rate of patients treated with ECMO in our hospital was 50.0%, in which circulatory support and respiratory support were 33.3% and 50.0%, respectively. The incidence rate of complications was 37.8%. The lactic acid level in arteries blood before ECMO was an influencing factor of prognosis, and had significant statistical difference between survival and dead patients(P<0.05). Conclusion ECMO is an effective therapy method for critical patients with respiratory or circulatory failure, but it still has many clinical difficulties and complications. Taking the right time and decreasing the occurrence of complication is important.
    Feasibility of 3D print individual template combined with CT-guided 125I seed implantation for malignant tumors
    ZHANG Ying, LIN Qi, YUAN Yuan, DAI Jianjian, GENG Baocheng, XU Ruicai, LIU Yakun, HAN Mingyong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  44-50.  doi:10.6040/j.issn.1671-7554.0.2016.1157
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    Objective To evaluate the feasibility of 3D print individual template and CT-guided 125I seed implantation for malignant tumors. Methods A total of 30 patients were involved in this study. Before 125I seed implantation, the patients received CT simulation orientation as the predicted posture. The individual template was designed by the brachytherapy treatment planning system(BTPS), and printed by 3D print technique. Every patient received 125I seed implantation under the guidance of 3D print individual template and CT scan. After implantation, treatment quality was evaluated with CT and BTPS. Results The 3D print individual templates were successfully designed and manufactured. Of all 30 cases, 28 were successfully implanted with the 3D print templates, and 2 failed. Of the 28 successful cases, 22 had the same number of seeds implanted as scheduled before operation, and the other 6 cases optimized the number of seeds during operation. Postoperative evaluation indicated that 14(50.0%)cases had excellent implants, 10(35.7%)had good implants, 4(14.3%)had suboptimal implants, and no case had poor implants. Conclusion The 3D print individual template and CT-guided 125I seed implantation appears to be a feasible, safe and effective modality for the treatment of malignant tumors.
    Effect of exenatide on serum chemerin level and liver fat contents in type 2 diabetes mellitus combined with non-alcoholic fatty liver disease patients
    YU Ning, GAO Yanyan, XIAN Yuxin, NIU Jiapeng, LI Li, WANG Jing, CAO Caixia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  51-55.  doi:10.6040/j.issn.1671-7554.0.2016.736
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    Objective To evaluate the effects of exenatide on the plasma glucose, liver fat content and serum chemerin in type 2 diabetic(T2DM)combined with non-alcoholic fatty liver disease(NAFLD)patients. Methods Sixty patients with T2DM and NAFLD were randomly divided into exenatide group(n=30)and control group(n=30). Exenatide group was treated by exenatide(10 μg subcutaneous injection one hour before breakfast and dinner), and the control group was treated by insulin glargine(the starting dose was 0.2 U/kg or 6-10 U)for 12 weeks. The changes of body weight(BW), body mass index(BMI), fasting plasma glucose(FPG), glycosylated hemoglobin(HbA1c), blood lipid(including TG, TC, HDL and LDL), insulin resistance index(HOMA-IR), liver enzyme index(including ALT and AST)and serum chemerin levels were measured, and the change of liver fat content was quantitated by ultrasound. Results Treated for 12 weeks, the levels of BW, BMI, WHR, HbA1c, FPG, TG, 2hPG, ALT, AST in exenatide group were significantly declined(P<0.05, P<0.01), the levels of FINS, HOMA-IR, chemerin and LFC decresed(P<0.01); the levels of HbA1c and FPG decresed in control group(P<0.01). Correlation analysis showed that BMI, TG, HOMA-IR and chemerin were associated with LFC; the decrease of TG and HOMA-IR were 山 东 大 学 学 报 (医 学 版)54卷11期 -于宁,等.艾塞那肽对2型糖尿病合并非酒精性脂肪肝患者肝脏脂肪含量及血清chemerin水平的影响 \=-independently impacting factors for LFC by multiple stepwise regression analysis(β=1.614, 1.360, P<0.05). Conclusion Exenatide can improve the glucolipid metabolism and liver function, and reduce BW, LFC and serum chemerin levels, which may be the effect drugs for the treatment of T2DM combined with NAFLD.
    Comparison of the adverse events of percutaneous pedicle screw fixation and traditional open pedicle screw fixation for thoracolumbar fractures: a Meta-analysis
    SUN Xiangyao, HAI Yong, ZHANG Xinuo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  56-63.  doi:10.6040/j.issn.1671-7554.0.2016.901
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    Objective To systematically review the adverse events of percutaneous pedicle screw fixation(PPSF)and traditional open pedicle screw fixation(TOPSF)for thoracolumbar fracture, in order to provide more evidence for the effective treatment of thoracolumbar fracture. Methods Databases including Pubmed, Cochrane Central Register of Controlled Trails(CENTRAL), and Embase were retrieved with computer. Journal of Bone and Joint Surgery, Spine, 山 东 大 学 学 报 (医 学 版)54卷11期 -孙祥耀,等.经皮内固定术与传统后路开放内固定术治疗胸腰椎骨折不良事件比较的Meta分析 \=-and European Spine Journal were manually searched. The searching terms were thoracolumbar fracture, lumbar fracture, percutaneous pedicle screw fixation, open fixation, posterior surgery, sextant, traditional fixation, conventional fixation, minimally invasive surgery, comparative study, randomized controlled trail, and clinical trail. Data were then screened, extracted, assessed, and analyzed with RevMan 5.3 software. Results A total of 18 studies including 4 randomized controlled trials, 3 prospective comparative studies, 11 retrospective comparative studies were enrolled, involving 1,034 patients. Meta-analysis showed that the PPSF group, compared with the TOPSF group, had shorter surgery time(WMD=-0.95, 95%CI: -1.33 - 0.57, P<0.001), less intraoperative blood loss(WMD=-2.97, 95%CI: -3.69 - 2.25, P<0.001), less postoperative drainage amount(WMD=-2.43, 95%CI: -3.04 - 1.83, P<0.001), shorter hospital stay(WMD=-5.37, 95%CI: -6.69 - 4.05, P<0.001), less time from surgery to walking(WMD=-2.51, 95%CI: -3.66 - 1.36, P<0.001), less overall postoperative visual analogue scale(WMD=-1.14, 95%CI: -1.62 - 0.66, P<0.001), lower postoperative Oswestry disability index(WMD=-1.78, 95%CI: -3.00 - 0.55, P=0.004), less postoperative Cobb angle correction loss(WMD=-0.52, 95%CI: -0.93 - 0.11, P=0.01), longer intraoperative exposure to radiation(WMD=5.12, 95%CI: 2.89-7.34, P<0.001). However, no significant difference was found between the two groups with regard to the rate of screw misplacement(OR=1.04, 95%CI: 0.43-2.56, P=0.92)and the rate of infection(OR=0.39, 95%CI: 0.12-1.26, P=0.12). Conclusion PPSF can not only shorten surgery time, hospital stay, and time from surgery to walking, but also lessen intraoperative blood loss, postoperative drainage amount, postoperative visual analogue scale, postoperative Oswestry disability index, and postoperative Cobb angle correction loss, but achieve the same accuracy in pedicle screw placement as TOPSF and secure the operation. Meanwhile, PPSF can protect the surrounding soft tissues, and reduce the rate of adverse events.
    Contents and ratios of type I and type III collagens in normal skin and hypertrophic scar in people of different ages
    WANG Cheng, RONG Yanhua, SHEN Yuming, ZHANG Guoan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  64-67.  doi:10.6040/j.issn.1671-7554.0.2015.1043
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    Objective To quantify the contents of type I and III collagens and their ratios in normal skin and hypertrophic scar in people of different ages, and to explore the rule and cause of changes. Methods Normal human skin specimens and hypertrophic scar specimens were obtained from spontaneously aborted fetus and people of different ages, including the children and adolescent group(≤18 years), adult group(19-50 years), elderly group(>50 years). The total collagen contents were determined by hydroxyproline method. The ratios of types I and III collagens were examined by immunohistochemistry, and their contents were calculated. Results The difference of the contents of types I and III collagens and their ratios in normal skin were statistically significant among different age groups(P=0.01). Their ratios increased with the increase of age, and the differences were statistically significant among different age groups(P=0.02). The difference of contents of types I and III collagens and their ratios in hypertrophic scars were statistically significant among all groups, also between any two groups(P=0.03). In every age group, the difference of the contents of types I and III collagens and their ratios between normal skin and hypertrophic scar was statistically significant (P=0.02). Conclusion With age increasing, the changes of the ratios of types I and III collagens in the normal skin changes, which results in changes of the tension and elasticity of skin and the healing process after injury. The contents of types I and III collagens and their ratios significantly changes in hypertrophic scars, resulting in different structure of scar from normal skin.
    Introvoxel incoherent motion MR imaging for the assessment of renal function of diabetes mellitus
    YU Lu, LIU Qiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  68-71.  doi:10.6040/j.issn.1671-7554.0.2015.1065
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    Objective To investigate the value of introvoxel incoherent motion magnetic resonance imaging(IVIM-MRI)in evaluating renal function of diabetes mellitus. Methods IVIM-MRI was performed in 20 cases of diabetes mellitus(diabetic group)and 20 healthy controls(control group)using 3.0T MR system. The differences of diffusion coefficient(D), perfusion fraction(f), and pseudodiffusion coefficient(D*)were compared. Results D and f values of renal cortex of the diabetic group were significantly higher than those of the control group(t=2.187, P=0.035; t=2.334, P=0.025). D and f values of renal medulla of the diabetic group were higher than those of control group, but with no significant differences(t=1.753, P=0.088; t=0.682, P=0.499). D* values of renal medulla and cortex of the diabetic group were higher than those of the control group, but without significant differences(t=0.088, P=0.93; t=0.592, P=0.557). D, f, D* values of renal medulla were significantly different from those of renal cortex in both diabetic and control groups(diabetes mellitus group t=9.64, P=0.002; t=12.163, P=0.001; t=15.993, P=0.001; control group t=11.228, P=0.001; t=12.055, P=0.001; t=14.708, P=0.001). Conclusion Introvoxel incoherent motion MR imaging can evaluate the renal function of diabetic patients non-invasively.
    Diffusion-weighted MR neurography of the median nerve in carpal tunnel syndrome: a preliminary study
    BAO Hongjing, WANG Guangbin, WANG Shanshan, WU Chao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  72-75.  doi:10.6040/j.issn.1671-7554.0.2015.1087
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    Objective To compare the diagnostic value of diffusion-weighted MR neurography(DW-MRN)and ultrasonography of median nerve(MN)lesion in carpal tunnel syndrome(CTS). Methods A total of 13 healthy volunteers(19 wrists, control group)and 14 CTS patients(17 wrists, patient group)were included. The ultrasound(US)and DW-MRN were used to detect the MN at the level of pisiform bone. The cross-sectional area(CSA)and apparent diffusion coefficient(ADC)were calculated by two independent inspectors. Receiver operating characteristic(ROC)curve was drawn to assess the diagnostic efficiency. Results There were significant differences on the CSA and ADC between control group and patient group(both P<0.001). The area under curve(AUC)of CSA was higher than that of ADC in the diagnosis of CTS(AUC>0.8). No significant difference of diagnostic value was found between DW-MRN and US(P>0.05). Conclusion DW-MRN has good diagnostic efficiency for the MN in carpal tunnel syndrome, and has equal efficiency as US.
    Inhibitory control ability of ERP research on internet addiction disabilities based on personality types of traditional Chinese medicine
    ZHANG Bohua, WANG Yue, YANG Yongxin, SONG Jingjie, ZHANG Shengwei, HU Shuang, SHENG Jie, TAN Meng, FENG Ye
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  76-81.  doi:10.6040/j.issn.1671-7554.0.2015.1068
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    Objective To explore the component difference of event-related potentials(ERPs)of the control ability of different personality types of internet addiction interpersonal barriers patients, and to reveal the electrophysiological evidence as well as the effect on inhibitory control ability. Methods A total of 32 patients with internet addiction interpersonal barriers who had Yin(n=16)and Yang(n=16)personality were selected according to the Clinical Diagnostic Criteria for Internet Addiction, Interpersonal Comprehensive Diagnostic Scale and Five-pattern Personality Inventory in this study. The prefrontal executive control function of the two groups was tested with Go/Nogo paradigm, and the differences of ERPs were analyzed. Results The N2 and P3 waves were successfully induced in the two types of personalities. Analysis of the composition of P3 indicated that, Nogo stimulation evoked a larger amplitude and earlier latency of P3 than Go stimulation; Fcz electrode induced the most powerful P3, with the most obvious Nogo effect. The 山 东 大 学 学 报 (医 学 版)54卷11期 -张伯华,等.基于中医人格分型的网瘾交往障碍者抑制控制能力的ERP研究 \=-subjects with Yang personality had significant N2 Nogo amplitude effect, while subjects with Yin personality had not. Conclusion Compared with subjects with Yang personality, those with Yin personality have poorer inhibitory control function on monitoring of and response to conflicting information.
    Epidemiological characteristics of hemorrhagic fever with renal syndrome patients in Huangdao District of Qingdao City, China from March, 2012 to February, 2013
    ZHANG Zhentang, FANG Lizhu, XUE Zaifeng, ZHAO Li, MA Dongqiang, WEN Hongling, LIU Jianwei, ZHANG Xiaoshuang, YU Xuejie
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(11):  82-86.  doi:10.6040/j.issn.1671-7554.0.2015.777
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    Objective To investigate the incidence and epidemiological feature of hemorrhagic fever with renal syndrome(HFRS)from March, 2012 to February, 2013 in Huangdao District, Qingdao City and provide the data for the prevention and control of HFRS. Methods A total of 106 acute phase sera of suspected HFRS patients were collected from Huangdao District. These samples were analyzed for Hantavirus(HV)IgM antibodies and RNA through ELISA and nested RT-PCR respectively. HV RNA positive samples were used for genotypic assay. Results Twenty sera of one hundred and six acute phase sera were positive to HV-IgM antibody, and 2 sera were positive to Hantanvirus(HTN)RNA. One of the sera is positive validated by nested RT-PCR, as well as by ELISA. So the total positive number is 21, and the total positive rate was 19.8%. Among these patients, 14 were male and 7 were female, most of whom belonged to the 45-59 year-old group. The disease had mostly occurred in autumn with 16 positive cases and there were also sporadic cases in other seasons. Five of the twenty-one sera were positive in Zangnan County from March, 2012 to February, 2013 and other counties still had some cases. Conclusion In Huangdao District of Qingdao 山 东 大 学 学 报 (医 学 版)54卷11期 -张振堂,等.2012年3月~2013年2月青岛市黄岛区肾综合征出血热流行特征 \=-City, HFRS mainly occurrs in autumn and most patients are middle-age men.