Table of Content

  

10 December 2016
Volume 54 Issue 12

Construction and identification of lentiviral vector for angiopoietin-1 gene and its expression in human umbilical cord mesenchymal stem cells

LIU Ning, L(¨overU)Xin, LI Dong, HUNAG Zhiwei, LIU Yi, ZHANG Leling

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  1-7.  doi:10.6040/j.issn.1671-7554.0.2016.730

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Objective To construct the lentivirus vector carrying angiopoietin-1 gene(Ang-1), and to investigate its expression in human umbilical cord mesenchymal stem cells(hUC-MSCs)and the immunomodulatory effect on Ang-1-modified hUC-MSCs. Methods The cDNA encoding full-length human Ang-1 was amplified by RT-PCR from the total RNA of hUC-MSCs, and then subcloned to lentiviral vector backbone plasmid GV287. The recombinant plasmid GV287-Ang-1 was co-transfected with packaging and enveloping plasmids pHelper 1.0 and pHelper 2.0 into 293T cells. Vector titer was quantified using cell fluorescence. The transfection was detected by fluorescence microscopy, and protein expression of Ang-1 was detected by Western blotting. T lymphocytes proliferation was assessed by cell counting kit 8(CCK8). Results The recombinant lentiviral vector GV287-Ang-1 was verified correctly by PCR and DNA sequencing. The titer of concentrated virus was 2×10⁸ TU/mL, and the optimal MOI for transfecting hUC-MSCs was 8. Western blotting results showed that the Ang-1 expression of the GV287-Ang-1 transfected group was significantly higher 山 东 大 学 学 报 (医 学 版)54卷12期 -刘宁,等.人血管生成素1基因慢病毒表达载体的构建及其在脐带间充质干细胞的表达 \=-than those of the non-transfected group and GV287 group. The suppression ratio of T lymphocytes in Ang-1-UC-MSCs group was significantly increased as compared to that in the UC-MSCs group. Conclusion The lentivirus vector carrying human Ang-1 was constructed successfully and overexpressed effectively in hUC-MSCs. Ang-1-modified hUC-MSCs can enhance the immunosuppressive capability, which suggests a potential clinical application.

Effects of EGCG on the expressions of hippocampal amyloid beta protein and neprilysin enzyme in C57BL/6 young mice exposed to lead

YIN Yanyan, ZHAO Linyuan, ZHANG Xiaohuan, HUANG Hui, SUN Beibei, LI Wenjie

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  8-13.  doi:10.6040/j.issn.1671-7554.0.2015.1231

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Objective To explore the effects of different doses of epigallocatechin 3 gallate, table gallic catechin gallic acid ester(EGCG)on the learning and memory ability, amyloid beta and neprilysin enzymes in lactation mice which were exposed to lead. Methods SPF pregnant C57BL/6 mice were divided into control group(drank distilled water, n=4)and lead exposure group(drank 0.5% Pb-contaminated acetic acid solution, n=14). Another 40 male lead-exposed mice were divided into 4 groups, low EGCG group(1.5 mg/kg), which are moderate EGCG group(3.0 mg/kg), 山 东 大 学 学 报 (医 学 版)54卷12期 -尹延彦,等.EGCG对铅暴露C57BL/6仔鼠海马Aβ蛋白及NEP酶表达的影响 \=-high EGCG group(4.5 mg/kg)and lead exposure group, with 10 mice in each group. Another 10 male mice served as the control group(normal saline). After 21 days of treatment, all rats’ learning and memory ability was tested with Morris water maze experiment; the lead content in blood and hippocampus was determined with graphite furnace atomic absorption spectrometer; the protein contents of Aβ_1-40, Aβ_1-42, AβPP mRNA, AβPP and the neprilysin enzyme were also detected. Results The lead exposure group, and low, moderate and high EGCG groups had significantly higher content of blood lead than the control group(P<0.05). There was no difference among EGCG groups and lead exposure group(P=0.174, 0.086, 0.071). Lead exposure group had longer escaping latency than the control group(P<0.001), while the EGCG group had shorter escaping latency than the control group(P<0.001). Compared with the control group, the lead exposure group had increased content of Aβ_1-40 and Aβ_1-42(P<0.001), and decreased Nep content(P<0.001); the moderate and high EGCG groups had decreased Aβ_1-40 and Aβ_1-42 content and increased Nep content(P<0.001), and the high EGCG group had better interventional effect. Conclusion EGCG intervention can significantly improve the learning and memory ability of mice. EGCG can reduce Aβ_1-40 and Aβ_1-42 contents in the hippocampus, inhibit the expression of related gene AβPP, and upregulate the Nep protein expression.

miR-196a promotes cell growth of HBx-HepG2 via targeting SOCS6

YU Guifang, CHEN Shudi, CHEN Xuezhu, HOU Kailian, LIANG Min

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  14-19.  doi:10.6040/j.issn.1671-7554.0.2016.040

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Objective To explore the effect of miR-196a on cell growth in HBx-HepG2 cells, and to explore its underlying molecular mechanism. Methods The expressions of miR-196a and SOCS6 mRNA were detected with QRT-PCR, and the protein expression of SOCS6 was determined with Western blotting. The cell growth in HBx-HepG2 cells was evaluated with CCK-8 and colony formation assay. The target gene of miR-196a was validated with dual luciferase reporter experiment. Results The expression of miR-196a was significantly higher in HBx-HepG2 cells than in normal human liver cells(L02)(P<0.05). Over-expression of miR-196a enhanced cell growth in HBx-HepG2 cells(P<0.05), while knockdown of miR-196a suppressed cell growth(P<0.05). SOCS6 was downregulated in HBx-HepG2 cells compared with L02 cells(P<0.05), and miR-196a downregulated the expression of SOCS6 via targeting 3'UTR of SOCS6. Overexpression SOCS6 in HBx-HepG2 cells promoted cell growth. Conclusion miR-196a enhances cell growth via down-regulation of SOCS6 in HBx-HepG2 cells.

Cloning and functional characterization of an O-methyltransferase gene from Conocephalum conicum

LI Dandan, LIU Hui, CHENG Aixia

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  20-26.  doi:10.6040/j.issn.1671-7554.0.2016.1137

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Objective To isolate and characterize an O-methyltransferase(OMT)gene from Conocephalum conicum. Methods The full length cDNA sequence of CcOMT1 was obtained by RT-PCR and inserted into the prokaryotic expression plasmid pET32a, and then was transformed into Escherichia coli BL21(DE3)for expression. The recombinant protein was collected by Ni-NTA affinity column and purified for enzyme characterization. The analyses of multiple alignment and phylogenetic tree were performed using DNAMAN 7.0 and MEGA 5.1 softwares. Results Sequence analysis showed that the full length cDNA of CcOMT1 was 837 bp and encoded a 278-aa protein with a calculated molecular weight of 30.95 kDa and an isoelectric point of 5.59. The amino acid sequence of CcOMT1 showed 73.18%, 53.60%, and 44.60% identity with O-methyltransferase(OMT)from Plagiochasma appendiculatum, Medicago sativa and Mesembryanthemum crystallinum, respectively. The purified recombinant protein was able to methylate a variety of flavonoids and phenylpropanoids with aromatic vicinal dihydroxyl groups, and the preferred substrate was quercetin. Conclusion An OMT gene from Conocephalum conicum is cloned and characterized. The present investigation would provide a candidate gene for synthesis of methylated compounds at specific position through enzymatic method.

Effect of anticoagulation on the remodeling of residual false lumen after aortic valve replacement of DeBakey type I aortic dissection

WANG Long, ZHAO Xin, LI Yuanyuan, WANG Ximing, LI Decai, SONG Guangmin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  27-31.  doi:10.6040/j.issn.1671-7554.0.2016.871

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Objective To investigate the effect of anticoagulation on the remodeling of residual false lumen after aortic valve replacement of DeBakey type I aortic dissection. Methods Clinical data of 31 patients with DeBakey type I aortic dissection treated during Oct. 2014 and Jun. 2015 were retrospectively analyzed. The patients were divided into two groups, that is, group one(n=20)who underwent surgical repairs with total arch replacement and modified stented elephant trunk technique(Suns Operation), and group two(n=11)who underwent surgical repairs with Suns Operation and aortic valve replacement. About 3 to 6 months after surgery, aortic CT angiography was performed to measure the diameter of the true lumen and aorta in the level of the initial, middle and end of the stent, celiac trunk, superior mesenteric artery and renal artery in each phase. The pre-operation and postoperative ratios of true lumen and aorta were 山 东 大 学 学 报 (医 学 版)54卷12期 -王龙,等.主动脉瓣置换术后抗凝对DeBakeyⅠ型主动脉夹层残余假腔重塑效果的临床影响 \=-calculated, respec-tively. In addition, the false lumen thrombosis was observed. Results There was no significant difference in the preoperative ratio of the true lumen and aorta diameter in the levels of the initial, middle and end of the stent, celiac trunk, superior mesenteric artery and renal artery(P=0.30, 0.94, 0.96, 0.54, 0.83, 0.49)between the two groups. After surgery, there was no significant difference in the ratio of the true lumen and aorta diameter in the 6 levels(P=0.28, 0.16, 0.28, 0.39, 0.44, 0.23)between the two groups. Conclusion Whether the aortic valve is involved has no difference in the lesions of stent and distal aorta. Anticoagulation therapy after aortic valve replacement has no significant effect on the residual false lumen remodeling of DeBakey type I aortic dissection.

Relationship between circulating platelets microparticles and endothelial microparticls and prognosis in patients with acute ischemic stroke

QU Lixin, SHI Xinghua, DU Yifeng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  32-36.  doi:10.6040/j.issn.1671-7554.0.2016.831

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Objective To explore the relationship between circulating platelets microparticles(PMP)and endothelial microparticls(EMP)and prognosis in patients with acute ischemic stroke(AIS). Methods Fifty-six patients with AIS(AIS groups)hospitalized from March, 2013 to June, 2015 and 40 people who took health examination in Dezhou Peoples Hospital were selected(Control group), and all subjects were investigated to explore the risk factors of prognosis. Venous blood(5 mL)was extracted from each subject. Flow cytometry was used to detect the levels of PMP and EMP. Patients in AIS group were evaluated by the National Institutes of Health Stroke Scale(NIHSS)in admission and discharge, and were classified into two types, poor prognosis and favorable prognosis, according to the scores. Risk factors that affected prognosis were investigated by the Logistical regression analysis. Results The levels of PMP and EMP in AIS group were significantly higher than those in Control group(P<0.05). The levels of PMP and EMP in patients with favorable prognosis were significantly higher than those with poor prognosis(P<0.05). According to Logistical regression analysis, hypertension,diabetes and levels of circulating PMP and EMP were independent risk factors for the prognosis of AIS patients. Conclusion Circulating PMP and EMP levels are associated with AIS, which play important roles in the prognosis of AIS patients.

Correlation between serum prealbumin and neonatal behavioral neurological assessment of hypoxic-ischemic encephalopathy in neonates

ZHUANG Genmiao, TANG Ling, ZANG Lijiao, AN Li

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  37-40.  doi:10.6040/j.issn.1671-7554.0.2016.606

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Objective To analyze the association between serum prealbumin(PA)level and neonatal behavioral neurological assessment(NBNA)in neonates with hypoxic-ischemic encephalopathy(HIE). Methods A total of 24 neonates including 11 males and 13 females were selected from HIE newborns admitted to Jinan Central Hospital during Jan. 2014 and Dec. 2015. The neonates were divided into 3 groups according to the HIE diagnostic criteria and dinical grading in 2005, mild(n=11), moderate(n=9), and severe(n=4), and another 20 healthy newborns served as controls. Blood samples and NBNA scores were collected from all newborns on day 1, 3, and 10 after birth, to detect the serum PA values. Results The Bonferronis test indicated that on day 1 and 3, there existed such relationship among PA level of 4 groups: PA(severe)< PA(moderate)< PA(mild)< PA(control),(P<0.01), so did NBNA scores on day 1, 3 and 10(P<0.01). Besides, the Pearsons Correlation Coefficient test showed significant positive linear correlation between PA level and NBNA scores of each HIE group, on day 1(r=0.554, P<0.01), after treatment, and day 10(r=0.344, P<0.01). Conclusion As the clinical grading becomes higher, the serum 山 东 大 学 学 报 (医 学 版)54卷12期 -庄根苗,等.新生儿缺氧缺血性脑病中血清前白蛋白水平与新生儿行为神经测定的相关性 \=-PA values and the NBNA scores are lower. Along with the recovery of disease, the serum PA values and NBNA scores gradually increase. The serum PA combined with NBNA scoring could be used as the indicator of early diagnosis and prognostic evaluation of neonatal HIE.

A standard surgical protocol and outcome of treating varus posteromedial rotational instability of elbow

LIU Tao, BAO Feilong, GAO Wei, KANG Shijie, LÜ Fuxin, HU Yiming

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  41-45.  doi:10.6040/j.issn.1671-7554.0.2016.228

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Objective To explore a standard surgical protocol and observe the clinical outcomes of treating varus posteromedial rotational instability(VPRI)of the elbow. Methods A total of 15 VPRI cases surgically treated via the medial and lateral approach during Dec. 2011 and Nov. 2014 were involved, including 9 males and 6 females, aged 11 to 69 years, mean 35.6 years. Altogether 7 left and 8 right elbows were affected. Causes of injury included traffic injury in 1 case and fall on the ground from the standing position in 14 cases. O Driscoll classification showed 9 cases of type Ⅱ1, 5 cases of type Ⅱ2 and 1 case of type Ⅱ3. The elbow stability and the humeroradial joint space were assessed by applying varus, pronated and axial stresses onto the forearm under fluoroscopy after anesthesia pre-, intra- and postoperation. The fractures of the coronoid were fixed with mini-plates or special designed plates, combined with K-wires or lasso sutures when fracture comminuted or extended to tip, via the medial approach, then lateral Kocher approach was performed, and lateral collateral ligament(LCL)was repaired using suture anchors. No external fixator was used. Rehabilitation program was early initiated with the guidance of surgeons after operation. Results All cases were followed-up for 12 to 30 months(average 18.7 months). Bony union was achieved in all cases within 7 to 14 weeks, with an average of 10 weeks. The average flexion was(136.8°±3.3°), average extension(3.6°±1.3°), average pronation(86.8°±5.6°), average supination(88.6°±3.5°). No complications such as elbow instability, pain and cubitus varus occurred. According to the Mayo elbow performance score(MEPS), the results were excellent 山 东 大 学 学 报 (医 学 版)54卷12期 -刘涛,等.肘关节内翻后内侧旋转不稳的手术疗效 \=-in 13 cases and good in 2 cases. The mean DASH(disabilities of the arm, shoulder and hand)score was 5.3(2.5 to 12.5). Conclusion Using standard surgical protocol for elbow varus posteromedial rotational instability can restore sufficient elbow stability to allow early motion, enhance the functional outcome, and reduce the overall complications such as cubitus varus, early degenerative changes and instability.

Diagnostic value of pepsinogen in atrophic gastritis and gastric cancer evaluation

ZHANG Shoucai, GAO Yu, ZHENG Guixi, YANG Yongmei, LIU Yanhong, GAO Yang, WANG Chuanxin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  46-52.  doi:10.6040/j.issn.1671-7554.0.2016.1146

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Objective To evaluate the significance of pepsinogen in atrophic gastritis and gastric cancer using chemiluminesent immunoassay assay(CLIA)and enzyme linked immunosorbent assay(ELISA), and to analyze the consistency of the two methods. Methods CLIA and ELISA were used to determine the expressions of serum PG Ⅰ, PGⅡ and PG Ⅰ/Ⅱ(PGR)in healthy controls(n=95), non-atrophic gastritis cases(n=173), atrophic gastritis cases(n=407)and gastric cancer cases(n=135). The most suitable cut-off values of PG of the two methods were determined. Their diagnostic efficiencies were evaluated, and the consistency of CLIA and ELISA in detecting PG was analyzed. Results The expression of PG Ⅱ in gastric cancer group was higher than in atrophic gastritis group, non-atrophic gastritis group, and healthy controls(CLIA: P=0.027, 0.002, <0.001; ELISA: P=0.008, <0.001, <0.001). PGR in gastric cancer group was lower than in atrophic gastritis group, non-atrophic gastritis group, and healthy controls(CLIA: P<0.001, <0.001, <0.001; ELISA: P<0.001, <0.001, 0.001). PG Ⅱ>13.20 and PGR≤6.67 were considered the most suitable cut-off value for diagnosing gastric cancer using CLIA, and the sensitivity 山 东 大 学 学 报 (医 学 版)54卷12期 -张守彩,等.两种方法评估胃蛋白酶原在萎缩性胃炎和胃癌中的诊断价值 \=-and specificity were 80.88% and 71.64%, respectively. PG Ⅱ>18.10 and PGR≤6.18 were considered the most suitable cut-off value for diagnosing gastric cancer using ELISA, and the sensitivity and specificity were 80.14% and 62.72%, respectively. Further analysis showed that Kappa index of CLIA and ELISA in diagnosing gastric cancer and atrophic gastritis were 0.706 and 0.569, respectively. Conclusion PG Ⅱ and PGR can be used as useful biomarkers for the diagnosis of gastric cancer. The combination of PG Ⅱ and PGR has considerable diagnostic value of atrophic gastritis and gastric cancer with higher sensitivity and specificity. CLIA and ELISA have a good consistency in detecting PG.

Clinical efficacy of CT-guided ¹²⁵I seed brachytherapy in the treatment of metastatic hepatic carcinoma

FANG Feifei, ZHANG Ying, LIN Qi, YUAN Yuan, DAI Jianjian, XU Ruicai, GENG Baocheng, HAN Mingyong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  53-57.  doi:10.6040/j.issn.1671-7554.0.2016.1106

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Objective To evaluate the efficacy and complications of CT-guided permanent ¹²⁵I seed interstitial brachytherapy in the treatment of metastatic hepatic carcinoma. Methods A total of 22 patients with metastatic hepatic carcinoma were enrolled. A treatment plan was made for each patient using a computerized treatment planning system(TPS)to determine the dose of radioactive seeds implanted and the site. With CT guidance, ¹²⁵I seeds were placed into the metastatic sites in the liver. After operation, CT scan was performed to verify the position and intensity of ¹²⁵I seeds. CT scan was performed 1 month, 3, 6, 9 and 12 months after operation to evaluate the local control rate of tumor and complications. Results Altogether 27 liver lesions were included in this study. The median longest diameter was 3.4 cm(1.0-7.4 cm). The number of ¹²⁵I seeds was 4-87. The activity was 1.85×10⁷-2.96×10⁷ Bq(0.5-0.8 mCi). One month after operation, 11 patients gained complete remission(CR), 6 partial remission(PR), 5 stable disease(SD)and 5 progression of disease(PD). The local tumor control rate 1 month, 3, 6, 9 and 12 months after operation was 81.5%, 78.9%, 76.9%, 77.8% and 66.7%, respectively. During the follow-up of 1-14 months(median 6 months), 13 patients survived and 9 died. The median survival time for all patients was 12 months. The rate of pain relief was 91.7%. Minor complications were bleeding ［31.8%(7/22)］, nsusea/vomiting ［13.6%(3/22)］, 山 东 大 学 学 报 (医 学 版)54卷12期 -房菲菲,等.CT引导放射性¹²⁵I粒子植入治疗转移性肝癌的临床疗效 \=- fever［4.5%(1/22)］ and seed displacement ［9.1%(2/22)］. Conclusion CT-guided ¹²⁵I seed brachytherapy is a safe and effective treatment for metastatic hepatic carcinoma with few complications.

Role of vascular endothelial cell activation in the pathogenesis of post infectious irritable bowel syndrome

LI Qian, DAI Taoli, HAN Wei, GUO Chenghao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  58-61.  doi:10.6040/j.issn.1671-7554.0.2016.464

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Objective To investigate the morphological changes of vascular endothelial cells as well as changes in the level of plasma endothelin(ET)and nitric oxide(NO)in patients with post infectious irritable bowel syndrome(PI-IBS), in order to clarify the possible role of vascular endothelial cell activation in the pathogenesis of PI-IBS. Methods The mucosa biopsy samples and plasma were obtained from 28 PI-IBS patients and 12 healthy controls during the same period. The morphological changes of vascular endothelial cells were observed with light microscope. The plasma ET and NO levels in both groups were measured with radioimmunoassay and nitrate reductase method. Results There were more significant morphological changes in vascular endothelial cells in the PI-IBS patients than in the controls. The plasma ET and NO levels of the PI-IBS patients were(55.49±13.49)ng/L and(75.90±16.52)μmol/L, respectively, which were significantly higher than those of the controls［(43.40±13.69)ng/L and(62.48±15.63)μmol/L, both P<0.05］. Conclusion The vascular endothelial cell activation may be closely related to the pathogenesis of PI-IBS.

Clinical research of stent placement by electronic bronchoscopy on malignant airway stenosis with one side lung ventilation

WEN Fangjing, SHI Xiaowu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  62-66.  doi:10.6040/j.issn.1671-7554.0.2015.1364

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Objective To evaluate the value of airway stent placement by electronic bronchoscopy on malignant airway stenosis with one side lung ventilation. Methods Thirty-four patients in the Department of Respiratory Disease of Central Hospital of Wuhan from January 2011 to February 2015 were included. Airway stent was used to evaluate the short-term therapeutic effects, airways opening degree, pulmonary functions and complications. Results Follow up for one month, the symptoms of dyspnea were relieved. Airway diameter, dyspnea score, forced expiratory volume in one second(FEV1), partial pressure of oxygen(PaO₂), partial pressure of carbon dioxide(PaCO₂)were improved significantly(P<0.05). Adverse reactions in 2 weeks were cough(7 cases, 20.59%), sore throat(9 cases, 26.47%), and hemoptysis(10 cases, 29.41%). With the follow-up for one month,airway granulation proliferation occurred in 3 patients(8.82%), sputum drainage difficulty in 5 patients(14.71%), and 1 patient(2.94%)died because of massive hemoptysis after 27 days. Conclusion Stent placement by electronic bronchoscopy for malignant airway stenosis with one side lung ventilation is effect and safe, worthy to be promoted clinically.

Detection of peripheral thrombomodulin and myeloperoxidase and their significance in adults with obstructive sleep apnea hypopnea syndrome

ZHANG Hongxia, XU Yonghong, CHEN Li, GONG Huicheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  67-71.  doi:10.6040/j.issn.1671-7554.0.2016.645

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Objective To investigate the detection and significance of serum thrombomodulin(TM)and myeloperoxidase(MPO)in adults with obstructive sleep apnea hypopnea syndrome(OSAHS). Methods A total of 90 OSAHS patients treated during Mar. 2014 and Feb. 2016 were divided into 3 groups according to apnea hyponea index(AHI): mild group(n=30), moderate group(n=30)and severe group(n=30). Another 30 healthy people served as the control group. The peripheral blood was collected from the patients with fasting blood in the morning after patients received polysomnography(PSG). The serum TM and MPO levels were determined by enzyme-linked immunosorbent assay(ELISA). Patients in the severe group were treated with continuous positive airway pressure(CPAP)and surgery. After 6-8 days, the narrow airway plane was evaluated to determine appropriate approaches of surgery. TM, 山 东 大 学 学 报 (医 学 版)54卷12期 -张红霞,等.成人阻塞性睡眠呼吸暂停低通气综合征外周血TM、MPO的检测及意义 \=- MPO and PSG were repeatedly detected and analyzed. Results The serum TM and MPO levels were positively correlated with the severity of OSAHS(P<0.05). There was no significant difference in TM and MPO levels between the mild OSAHS group and control groups(P>0.05), but there was difference between the moderate and control groups, and between severe and control groups(P<0.05). The BMI of the severe group increased significantly compared with that of the mild, moderate and control groups(P<0.05). There was no correlation between TM and MPO levels and BMI, age and sex(P>0.05). There was positive correlation between TM and MPO levels and AHI(P<0.05), and negative correlation with LsaO₂(P<0.05). In the severe OSAHS group, LsaO₂ significantly increased after comprehensive treatment, while the TM and MPO levels and AHI significantly decreased(P<0.05). Conclusion Increased peripheral TM and MPO levels are involved in the cardiovascular damage in OSAHS patients. Detection of TM and MPO levels helps to determine the patients condition and efficacy of treatment, and assess the risk of cardiovascular disease.

Ultra-high-frequency ultrasound vs high-frequency ultrasound in assessing superficial infantile hemangioma

WU Yiliang, ZHANG Linfeng, LI Yuan, LIU Xiaowen, XU Guangqi, GAO Feng, LÜ Renrong, HUO Ran

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  72-76.  doi:10.6040/j.issn.1671-7554.0.2016.192

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Objective To compare the efficacy of 22 MHz ultra-high-frequency ultrasound and 13 MHz high-frequency ultrasound in assessing the clinical condition of superficial infantile hemangiomas. Methods We collected the two-dimensional ultrasound images and color Doppler flow images of the same section of 33 patients whose tumors depth was less than 1cm with 22 MHz and 13 MHz ultrasound separately, selected the 1 cm×1 cm areas just under the probe from these color Doppler flow images and analyzed them by software Image Magick. After that, we classified these two-dimensional ultrasound images according to whether the boundary of the tumors could be recognized and compared the results. We then separated every color Doppler flow image into two parts from the depth of 0.5cm to compare the two types of ultrasounds’ ability to display the blood flow. Results 22 MHz ultra-high-frequency ultrasound was inferior to 13 MHz high-frequency ultrasound in distinguishing the boundary of tumors(χ2=0.44, P=0.51). There was no difference between the two types of ultrasounds in identifying the depth of tumors in color Doppler flow images(0-0.5 cm: t=-9.38, P<0.05; 0.5-1.0 cm: t=4.59, P<0.05). There was no difference in displaying total blood 山 东 大 学 学 报 (医 学 版)54卷12期 -吴义良,等.超高频超声与高频超声在浅表性婴幼儿血管瘤中的应用 \=-flow(t=-1.63, P=0.11). Conclusion Compared with 13 MHz high-frequency ultrasound, 22 MHz ultra-high-frequency ultrasound can provide clearer two-dimensional ultrasound images and color Doppler flow images with more blood flow about a superficial infantile hemangioma when the depth of the tumor is less than 0.5 cm.

Seasonal characteristics of hand, foot and mouth disease in Shandong Province during 2008 and 2014 analyzed with concentration and circular distribution methods

LIU Hongyang, XIAO Jinhuan, WANG Fan, YANG Guang, LIU Hongqing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  77-81.  doi:10.6040/j.issn.1671-7554.0.2016.469

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Objective To explore the seasonal characteristics of hand, foot and mouth disease(HFMD)in Shandong Province from 2008 to 2014. Methods The surveillance data of HFMD in 2008-2014 were collected from the notifiable infectious disease reporting system of Shandong Province to analyze the seasonal characteristics. Results The average annual growth rate of HFMD was 22.96%, rising from 35.02/10 million to 121.01/10 million. Of the total reports, the number of cases reported in May to July accounted for 51.29%(188564/367655). The M value was 0.42, indicating that HFMD had a seasonal tendency. The circular distribution showed γ value was 0.42, (-overα)=185.196° ［(110.216°, 260.176°), P<0.01］, indicating that the peak day was in June 8th, and the epidemic peak was March 22nd to August 24th. There was statistically significant difference in the average angle during 2008 and 2014(F=2 401.14, P<0.01), indicating that the peak day was different in each year. The M value was highly correlated with γ value(r=1.000, P<0.01). Conclusion Hand, foot and mouth disease has a certain seasonal characteristics in Shandong Pro-vince from 2008 to 2014. The epidemic peak is March to August, and the duration of each year is different.

Analysis on the relationship between urine pH and metabolic syndrome

XU Yibo, JI Xiaokang, LI Xiangyi, SHEN Zhenwei, XUE Fuzhong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2016, 54(12):  82-85.  doi:10.6040/j.issn.1671-7554.0.2016.307

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Objective To investigate the correlation of urine pH with metabolic syndrome(MetS). Methods In this study, a longitudinal cohort with 8 720 participants was established based on the routine health check-up systems in Health Management Center of Shandong Provincial Hospital. Cox proportional-hazards model was used to detect the association between urine pH and MetS. Results 1 657 MetS occurred over an average 3.03 years follow-up, leading to a total incidence density of 62.79 per 1 000 person-years(1 657/26 389 person-years). Cox regression analysis by adjusting for age showed that hazard ratio(HR)value of lower urine pH group on MetS was1.36(95%CI: 1.21-1.53, P<0.05). After adjusting for age and four MetS components(hypertension, hyperglycemia, dyslipidemia and obesity)the HR was 1.20(95%CI: 1.07-1.35, P<0.05). After further adjusting for diet, smoking and drinking, the HR was 1.17(95%CI: 1.05-1.32, P<0.05). Conclusion Lower urine pH is an individual risk factor of MetS.