JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (12): 14-19.doi: 10.6040/j.issn.1671-7554.0.2016.040

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miR-196a promotes cell growth of HBx-HepG2 via targeting SOCS6

YU Guifang, CHEN Shudi, CHEN Xuezhu, HOU Kailian, LIANG Min   

  1. Department of Oncology, Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou 510700, Guangdong, China
  • Received:2016-01-12 Online:2016-12-10 Published:2016-12-10

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Abstract: Objective To explore the effect of miR-196a on cell growth in HBx-HepG2 cells, and to explore its underlying molecular mechanism. Methods The expressions of miR-196a and SOCS6 mRNA were detected with QRT-PCR, and the protein expression of SOCS6 was determined with Western blotting. The cell growth in HBx-HepG2 cells was evaluated with CCK-8 and colony formation assay. The target gene of miR-196a was validated with dual luciferase reporter experiment. Results The expression of miR-196a was significantly higher in HBx-HepG2 cells than in normal human liver cells(L02)(P<0.05). Over-expression of miR-196a enhanced cell growth in HBx-HepG2 cells(P<0.05), while knockdown of miR-196a suppressed cell growth(P<0.05). SOCS6 was downregulated in HBx-HepG2 cells compared with L02 cells(P<0.05), and miR-196a downregulated the expression of SOCS6 via targeting 3'UTR of SOCS6. Overexpression SOCS6 in HBx-HepG2 cells promoted cell growth. Conclusion miR-196a enhances cell growth via down-regulation of SOCS6 in HBx-HepG2 cells.

Key words: miR-196a, HBx-HepG2 cells, Suppressor of cytokine signaling 6, Cell growth

CLC Number: 

  • R574
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