JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (11): 7-14.doi: 10.6040/j.issn.1671-7554.0.2017.219

Previous Articles     Next Articles

Effects of high exposure of endogenous reproductive hormones on perimenopausal and postmenopausal blood pressure in rats

JIA Jiajing1, WANG Zhiping2   

  1. 1.Department of Epidemiology;
    2. Department of Labor Hygiene and Occupational Diseases, School of Public Health, Shandong University, Jinan 250012, Shandong, China
  • Received:2017-03-16 Online:2017-11-10 Published:2017-11-10

PDF (PC)

172

Abstract: Objective To explore the effects of high-dose endogenous estrogen(17-β E2)and progesterone(P)of the reproductive phase on the blood pressure of perimenopausal and postmenopausal rats and to observe the role of angiotensin II type-1 receptor(AT1R). Methods Bilateral ovariectomy was performed during the diestrus phase of estrus cycle to induce rat models of artificial estrous cycle by 17-β E2 and P. The models were divided into 3 groups: high-dose estrogen plus low-dose progesterone group(n=20, HELP), high-dose progesterone plus low-dose estrogen group(n=20, HPLE), and ovariectomized group(n=20, OVX). The experimental animals were given hormones for 8 weeks, decreasing dosages for 5 weeks and withdrawal hormones for 6 weeks to imitate the reproductive phase, perimenopausal phase and postmenopausal phase respectively. The changes of blood pressure, serum E2 and P, and protein and mRNA expressions of AT1R in the abdominal aorta were monitored. Results Compared with the OVX group, the HELP and HPLE groups had higher systolic blood pressure(SBP)in all phases(P<0.05); the HELP group had higher diastolic blood pressure(DBP)in the perimenopausal and postmenopausal phases(P<0.05), and higher protein and mRNA expressions of AT1R in the reproductive and perimenopausal phases(P<0.05). The protein expression of AT1R was positively correlated with serum concentration of E2 and SBP(P<0.01). Conclusion Exposure to excessively high-dose 山 东 大 学 学 报 (医 学 版)55卷11期 -贾佳静,等.育龄期高水平生殖激素对大鼠围绝经期及绝经后血压的影响 \=-endogenous estrogen in the reproductive phase raises the blood pressure in perimenopausal and postmenopausal rats. Elevated protein expression of AT1R in the abdominal aorta is correlated with high SBP.

Key words: Progesterone, Blood pressure, Postmenopausal phase, Estrogen, Reproductive phase, Angiotensin II type-1 receptor

CLC Number: 

  • R574
[1] Rapsomaniki E, Timmis A, George J, et al. Blood pressure and incidence of twelve cardiovascular diseases: lifetime risks, healthy life-years lost, and age-specific associations in 1.25 million people[J]. Lancet, 2014, 383(9932): 1899-1911.
[2] Barton M, Meyer MR. Postmenopausal hypertension: mechanisms and therapy[J]. Hypertension, 2009, 54(1): 11-18.
[3] Robitaille C, Dai S, Waters C, et al. Diagnosed hypertension in Canada: incidence, prevalence and associated mortality[J]. CMAJ, 2012, 184(1): E49-56.
[4] Zhou Y, Zhou X, Guo X, et al. Prevalence and risk factors of hypertension among pre- and post-menopausal women: a cross-sectional study in a rural area of northeast China[J]. Maturitas, 2015, 80(3): 282-287.
[5] Taddei S. Blood pressure through aging and menopause[J]. Climacteric, 2009, 12(Suppl 1): 36-40.
[6] Wenner MM, Stachenfeld NS. Blood pressure and water regulation: understanding sex hormone effects within and between men and women[J]. J Physiol, 2012, 590(23): 5949-5961.
[7] Son MK, Lim NK, Lim JY, et al. Difference in blood pressure between early and late menopausal transition was significant in healthy Korean women[J]. BMC Womens Health, 2015, 15: 64-71. doi: 10.1186/s12905-015-0219-9
[8] Rossouw JE, Anderson GL, Prentice RL, et al. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results From the Womens Health Initiative randomized controlled trial[J]. JAMA, 2002, 288(3): 321-333.
[9] Grady D, Herrington D, Bittner V, et al. Cardiovascular disease outcomes during 6.8 years of hormone therapy: Heart and Estrogen/progestin Replacement Study follow-up(HERS II)[J]. JAMA, 2002, 288(1): 49-57.
[10] Issa Z, Seely EW, Rahme M, et al. Effects of hormone therapy on blood pressure[J]. Menopause, 2015, 22(4): 456-468.
[11] Ryan J, Stanczyk FZ, Dennerstein L, et al. Hormone levels and cognitive function in postmenopausal midlife women[J]. Neurobiol Aging, 2012, 33(7): 1138-1147.
[12] Wharton W, Gleason CE, Olson SR, et al. Neurobiological underpinnings of the estrogen-mood relationship[J]. Curr Psychiatry Rev, 2012, 8(3): 247-256.
[13] 周宜, 陈钢, 夏丽娜, 等. 外源雌孕激素建立人工性周期大鼠模型的探索[J]. 中药药理与临床, 2007, 23(1): 76-77. ZHOU Yi, CHEN Gang, XIA Lina, et al. An exploration method of establishing an rat artificial estrous cycle from exogenous estrogen and progesterone[J]. Pharmacology and Clinics of Chinese Materia Medica, 2007, 23(1): 76-77.
[14] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C)method[J]. Methods, 2001, 25(4): 402-408.
[15] Oneda B, Cardoso CG., Jr, Forjaz CL, et al. Effects of estrogen therapy and aerobic training on sympathetic activity and hemodynamics in healthy postmenopausal women: a double-blind randomized trial[J]. Menopause, 2014, 21(4): 369-375.
[16] Brunt VE, Miner JA, Kaplan PF, et al. Short-term administration of progesterone and estradiol independently alter carotid-vasomotor, but not carotid-cardiac, baroreflex function in young women[J]. Am J Physiol Heart Circ Physiol, 2013, 305(7): H1041-1049.
[17] Anderson GL, Limacher M, Assaf AR, et al. Effects of conjugated equine estrogen in postmenopausal women with hysterectomy: the Womens Health Initiative randomized controlled trial[J]. JAMA, 2004, 291(14): 1701-1712.
[18] Boardman HM, Hartley L, Eisinga A, et al. Hormone therapy for preventing cardiovascular disease in post-menopausal women[J]. Cochrane Database Syst Rev, 2015, 10(3): 22-29.
[19] Subramanian M, Balasubramanian P, Garver H, et al. Chronic estradiol-17beta exposure increases superoxide production in the rostral ventrolateral medulla and causes hypertension: reversal by resveratrol[J]. Am J Physiol Regul Integr Comp Physiol, 2011, 300(6): R1560-1568.
[20] Subramanian M, Hahn-Townsend C, Clark KA, et al. Chronic estrogen exposure affects gene expression in the rostral ventrolateral medulla of young and aging rats: Possible role in hypertension[J]. Brain Res, 2015, 1627(19): 134-142.
[21] Zimmerman MA, Hutson DD, Trimmer EH, et al. Long- but not short-term estradiol treatment induces renal damage in midlife ovariectomized Long-Evans rats[J]. Am J Physiol Renal Physiol, 2017, 312(2): F305- F311.
[22] Reslan OM, Yin Z, do Nascimento GR, et al. Subtype-specific estrogen receptor-mediated vasodilator activity in the cephalic, thoracic, and abdominal vasculature of female rat[J]. J Cardiovasc Pharmacol, 2013, 62(1): 26-40.
[23] Koganti S, Snyder R, Thekkumkara T. Pharmacologic effects of 2-methoxyestradiol on angiotensin type 1 receptor down-regulation in rat liver epithelial and aortic smooth muscle cells[J]. Gend Med, 2012, 9(2): 76-93.
[24] Kafami M, Hosseini M, Niazmand S, et al. Interaction of central Angiotensin II and estrogen on systolic blood pressure in female DOCA-salt treated rats[J]. Adv Biomed Res, 2016, 5: 78-85. doi: 10.4103/2277-9175.180990
[25] Ozdemir Kumral ZN, Kolgazi M, Ustunova S, et al. Estrogen receptor agonists alleviate cardiac and renal oxidative injury in rats with renovascular hypertension[J]. Clin Exp Hypertens, 2016, 38(6): 500-509.
[26] Mompeon A, Lazaro-Franco M, Bueno-Beti C, et al. Estradiol, acting through ERalpha, induces endothelial non-classic renin-angiotensin system increasing angiotensin 1-7 production[J]. Mol Cell Endocrinol, 2016, 422(c): 1-8. doi: 10.1016/j.mce.2015.11.004
[27] Murase T, Hattori T, Ohtake M, et al. Effects of estrogen on cardiovascular injury in ovariectomized female DahlS.Z-Lepr(fa)/Lepr(fa)rats as a new animal model of metabolic syndrome[J]. Hypertension, 2012, 59(3): 694-704.
[28] Ricchiuti V, Lian CG, Oestreicher EM, et al. Estradiol increases angiotensin II type 1 receptor in hearts of ovariectomized rats[J]. J Endocrinol, 2009, 200(1): 75-84.
[29] Meng X, Dai X, Liao TD, et al. Dose-dependent toxic effects of high-dose estrogen on renal and cardiac injury in surgically postmenopausal mice[J]. Life Sci, 2011, 88(3-4): 178-186.
[30] Zhan E, Keimig T, Xu J, et al. Dose-dependent cardiac effect of oestrogen replacement in mice post-myocardial infarction[J]. Exp Physiol, 2008, 93(8): 982-993.
[31] Chidambaram M, Duncan JA, Lai VS, et al. Variation in the renin angiotensin system throughout the normal menstrual cycle[J]. J Am Soc Nephrol, 2002, 13(2): 446-452.
[32] Szmuilowicz ED, Adler GK, Williams JS, et al. Relationship between aldosterone and progesterone in the human menstrual cycle[J]. J Clin Endocrinol Metab, 2006, 91(10): 3981-3987.
[1] LUO Xiao, DAI Xiang, JIA Cunxian, SONG Huacui, GAO Lijie. Changing trend and influencing factors of blood pressure level among staff based on panel data model [J]. Journal of Shandong University (Health Sciences), 2022, 60(2): 109-114.
[2] Zhuxiu CHU,Wenjing ZHAO,Xiaoyan LI,Xiaoli KONG,Tingting MA,Liyu JIANG,Qifeng YANG. Significance of neoadjuvant chemotherapy and molecular marker changes in 218 women with breast cancer [J]. Journal of Shandong University (Health Sciences), 2021, 59(9): 130-139.
[3] DING Fei, JIANG Jie. Effects of curcumin on progesterone resistance in endometrial carcinoma [J]. Journal of Shandong University (Health Sciences), 2021, 59(4): 35-41.
[4] LIU Ran, TAO Guowei, ZHEN Junhui, LUO Xia, QIAN Richeng. Expression and significance of ER and PR in fibroids and myometrium during pregnancy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(5): 45-49.
[5] MA Huiming, ZHANG Yongfang, WANG Mengmeng, LI Xin, WANG Yongfeng, TIAN Hongcheng, HU Rong, WANG Yanrong, PEI Xiuying, XU Xian. Effect of estrogen on expression of estrogen receptor-β and forkhead transcription factor 3 in human granulosa cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(5): 50-55.
[6] XU Zhongyang, WANG Liqi, XU Zhenxing, ZHAO Qian, ZHU Shiming. Correlations among RhoA/ROCK signaling pathway, blood pressure variability and carotid artery intima-media thickness in essential hypertensive patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 48-51.
[7] DAI Hong, WANG Hong-li. Clinical observation of integrated traditional and western medicine in treatment of senile vaginitis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(S1): 30-31.
[8] FAN Xiao-yan1, YANG Chuan-hua2, LU Feng2, ZHANG Zhen-wei3. High-normal blood pressure epidemiological survey and analysis of influencing factors of the county authorities crowd in Shandong [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(11): 93-97.
[9] ZHAO Yong-hui1, Gao Jun2, LI Zhuo1, JIANG Yong2. Protective effects of progesterone on early brain injury in
rats after subarachnoid hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 55-.
[10] LIU Hai-rong1， YU Jin-ming2， LI Yan1， LIANG Jing1， LIU Xiao-lin1. Study of the relationship between ER， PR， C-erB-2 and
bone metastasis of  lung adenocacinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(4): 91-.
[11] CHENG Kai-hua1, ZHANG Li-xin2. Therapy and effect of GnHRa on sex hormone and bone mineral
density in patients with endometriosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(3): 83-86.
[12] FAN Lei1, LU Zi-long2, CHU Jie2, MA Ji-xiang2, SUN Xiao-yun3, HUANG Shuai1, DONG Jing2, GUO Xiao-lei2, ZHANG Ji-yu2. Evaluation of comprehensive intervention on blood pressure control in
 mid-western rural areas of Shandong Province [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 145-.
[13] LIU Min1,2， WANG Li2, ZHOU Ting2， RONG Feng-nian2.

Association between PR/AR gene polymorphisms and susceptibility to epithelial ovarian cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 144-148.

[14] LI Qun-feng. Effects of the estrogen receptor on stress-induced premature senescence of  vascular smooth muscle cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 13-16.
[15] LI Shu-ling1, XING Yi2, LI Yu-zhu1, GONG Li1, LI Hui3. Effect of Bu-Wang-San on estrogen and inflammatory  responses in ovariectomized rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 50-54.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] SHI Shuang, LI Juan, MI Qi, WANG Yunshan, DU Lutao, WANG Chuanxin. Construction and application of a miRNAs prognostic risk assessment model of gastric cancer[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 47 -52 .
[2] HUANG Fei,WANG Huaijing,XING Yi,GAO Wei,LI Yonggang,XING Ziying,LI Zhenzhong. Protective effects of NGF and GM1 on primary sensory neurons in SD rat with sciatic nerve injury[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(4): 332 -335 .
[3] LYU Longfei, LI Lin, LI Shuhai, QI Lei, LU Ming, CHENG Chuanle, TIAN Hui. Application of laparoscopic fine needle catheter jejunostomy in minimally invasive McKeown resection of esophageal cancer[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 77 -81 .
[4] SHAO Haigang, WANG Xuan, WANG Qing. Anatomy of the root canal system of mandibular first premolar in population of Shandong Province[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 85 -89 .
[5] JIANG Bao-dong,MA Xiang-xing,WANG Qing,WANG Qian,FENG Xiao-yuan,LI Ke,YU Fu-hua. Imaging parameters of multislice spiral CT venography in cerebral veins[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(11): 1084 -1086 .
[6] . [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(10): 1078 -1080 .
[7] TANG Fang1,2, ZHANG Yingqian3, WANG Zhiqiang4, KANG Dianmin4,
WANG Jiezhen1, XUE Fuzhong1. A 2D minimal spanning tree model of the spatial structures of natural focal disease[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(01): 106 -110 .
[8] LI Wei,LI Dao-wei,YE Qian,GAO Shun-cui,JIANG Shu-juan.

Diagnostic value of transbronchial needle aspiration in paratracheal mediastinal lesions

[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(11): 1063 -1065 .
[9] LI Yu-liang,WANG Yong-zheng,WANG Xiao-hua,ZHANG Fu-jun,ZHU Li-dong,ZHANG Wang-ming,LI Zheng,LI Zhen-jia,ZHANG Kai-xian. I implantation combined with Gemcitabine in the treatment of advanced pancreatic cancer[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(4): 393 -396 .
[10] WANG Xu-ping,ZHAO Ling,FENG Yu-xin,SHANG Lin-shan,LIU Jin-cheng,. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(6): 564 -567 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd